Ab221013

Manufactured by Abcam

Sourced in United Kingdom, China

Ab221013 is a monoclonal antibody primarily used for the detection and quantification of target proteins in various applications such as Western blotting, immunohistochemistry, and ELISA. It is a high-quality research tool that can be utilized in a range of scientific studies.

Automatically generated - may contain errors

Lab products found in correlation

Ab16051, by Abcam (1 mentions) Ab176660, by Abcam (1 mentions) Ab267646, by Abcam (1 mentions) Gsh px assay kit, by Nanjing Jiancheng (1 mentions) Sirna mate, by GenePharma (1 mentions) Ab176662, by Abcam (1 mentions)

2 protocols using ab221013

Fanconi Anemia Pathway Regulation

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CRA (98%) was purchased from Shanghai Acmec Biochemical Co., Ltd. (123-73-9, Shanghai, China), and HAECs were purchased from BeNa Culture Collection (BNCC337727; Beijing, China). Fanconi anemia complementation group A (FANCA) small interfering RNA (siRNA) and siRNA-Mate were synthesized and provided by GenePharma Co., Ltd. (G04002, Shanghai, China). Glutathione peroxidase (GSH-Px) assay kits were obtained from the Jiancheng Bioengineering Institute (A005; Nanjing, Jiangsu, China). Phospho-extracellular signal-regulated kinase (P-ERK, ab176660), phospho-c-Jun N-terminal kinase (P-JNK, ab176645), phospho-p38 kinase (ab221013), cyclooxygenase-2 (COX-2, ab267646), Wnt3a (ab54479), β-catenin (ab16051), phospho- ErbB2 (ab101229), and phospho-ErbB4 (ab61059) ELISA kits were purchased from Abcam (Cambridge, UK), and mitochondrial membrane potential assay kits were obtained from Abbkine Scientific Co., Ltd. (KTA4001; Wuhan, Hubei, China).

Xie M.Z., Liu J.L., Gao Q.Z., Bo D.Y., Wang L., Zhou X.C., Zhao M.M., Zhang Y.C., Zhang Y.J., Zhao G.A, & Jiao L.Y. (2022). Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde. Clinical Proteomics, 19, 33.

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Tumor Tissue MMP7, HMGB1, and JNK/p38 Analysis

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The tumor tissue from each group of nude mice was made into tissue homogenate. By following the ELISA kit instructions, the expression of Matrix Metallopeptidase 7 (MMP7) and High-Mobility Group Box 1 (HMGB1), and the phosphorylation levels of JNK and p38 in tumor tissues were detected. ELISA kits used in the experiment were MMP7 (No: JL29243, Shanghai Jianglai Industrial Co., Ltd., Shanghai, China), HMGB1 (JL51819, Shanghai Jianglai Industrial Co., Ltd.), JNK and p-JNK (ab176662, Abcam), p38 and p-p38 (ab221013, Abcam) kits.

Yin Y., Xie J., Peng F., Tan L., Xiao Y., Zheng H., Yin L., Situ H, & Zhang S. (2023). The topoisomerase inhibitor CPT-11 prevents the growth and metastasis of lung cancer cells in nude mice by inhibiting EGFR/MAPK signaling pathway. Heliyon, 9(5), e15805.

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