Solvent and reagents were purchased from Sigma-Aldrich and dried before use. The melting point was finding a digital Gallenkamp (SANYO) model MPD BM 3 apparatus and was uncorrected. 1H NMR and 13C NMR spectra were determined in CDCl3 or acetone-d6 solutions at 300 MHz and 75.4 MHz, respectively using a Bruker AM-300 spectrophotometer. FTIR spectra were recorded using an FTS 3000 MX spectrophotometer. The chemical shifts were measured in ppm units and coupling constants (J) in hertz.
Am 300 spectrophotometer
Manufactured by Bruker
Sourced in United States
The AM-300 spectrophotometer is an analytical instrument designed for measuring the absorption or transmission of light by a sample. It is capable of providing accurate and reliable measurements across a wide range of wavelengths, enabling the analysis of various materials and compounds.
Automatically generated - may contain errors
Lab products found in correlation
183 chn analyzer, by Leco (2 mentions)
Mpd bm 3.5 apparatus, by Sanyo (2 mentions)
Melting point apparatus, by Gallenkamp (2 mentions)
183 chns analyzer, by Leco (2 mentions)
Ftir spectrophotometer, by Bruker (1 mentions)
Dmso d6, by Bruker (1 mentions)
Mushroom tyrosinase ec 1.14.18.1, by Merck Group (1 mentions)
Alpha ftir spectrometer, by Bruker (1 mentions)
Api 2000 mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Bx 2 spectrometer, by PerkinElmer (1 mentions)
Nicolet is10 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Nicolet is10 spectrophotometer, by Bruker (1 mentions)
Mpd bm 3, by Sanyo (1 mentions)
Ifs 66v spectrometer, by Bruker (1 mentions)
Tga 50 unit, by Shimadzu (1 mentions)
Equinox 55 ft ir spectrometer, by Bruker (1 mentions)
12 protocols using am 300 spectrophotometer
1
Spectroscopic Characterization of Synthesized Compounds
2
Analytical Characterization of Synthesized Compounds
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Infrared spectroscopy
was performed using a FT-IR Thermo Scientific
NICOLET IS10 spectrophotometer. 1HNMR and 13CNMR spectra (Bruker AM-300 spectrophotometer) were recorded using
DMSO and chloroform as solvents (deuterated), and the chemical shift
data were stated as delta values related to tetramethylsilane (TMS).
A digital Gallenkamp melting point apparatus was used to record the
melting points of synthesized products and were uncorrected. All the
compounds synthesized were purified by recrystallization in a suitable
solvent. All the starting materials, reagents, and solvents used were
purchased from Sigma-Aldrich and were of high-purity grade. Elemental
analysis was performed using a LECO-183 CHN analyzer. All the reactions
were monitored by performing thin layer chromatography (TLC) on silica
gel plates and detected under ultraviolet (UV) light.
was performed using a FT-IR Thermo Scientific
NICOLET IS10 spectrophotometer. 1HNMR and 13CNMR spectra (Bruker AM-300 spectrophotometer) were recorded using
DMSO and chloroform as solvents (deuterated), and the chemical shift
data were stated as delta values related to tetramethylsilane (TMS).
A digital Gallenkamp melting point apparatus was used to record the
melting points of synthesized products and were uncorrected. All the
compounds synthesized were purified by recrystallization in a suitable
solvent. All the starting materials, reagents, and solvents used were
purchased from Sigma-Aldrich and were of high-purity grade. Elemental
analysis was performed using a LECO-183 CHN analyzer. All the reactions
were monitored by performing thin layer chromatography (TLC) on silica
gel plates and detected under ultraviolet (UV) light.
3
Characterization of Organic Compounds
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Melting points were recorded using a digital Gallenkamp (SANYO) model MPD BM 3.5 apparatus and are uncorrected. 1H NMR spectra were determined as CDCl3 solutions at 300 MHz using a Bruker AM-300 spectrophotometer. FT IR spectra were recorded using an FTS 3000 MX spectrophotometer, Mass Spectra (EI, 70 eV) on a GC-MS instrument Agilent technology, and elemental analyses were conducted using a LECO-183 CHNS analyzer. Both compounds were recrystallized from aqueous ethanol.
4
Thermal, Spectral, and NMR Analysis
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Melting points were evaluated using the validated digital Gallenkamp (SANYO) model MPD BM 3 equipment. The FTIR spectra were recorded using an FTS 3000 MX spectrophotometer. 1H NMR analysis was done in acetone-d6 solutions at 300 MHz and 75.4 MHz, respectively, using a Bruker AM-300 spectrophotometer. The chemical shifts were measured in ppm units and coupling constants (J) in hertz.
5
Synthesis and Characterization of Novel Compounds
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All the research chemicals were purchased from Daejung (South Korea), Sigma-Aldrich (St. Louis, MO, USA), and Alfa-Aesar (Germany). Digital Gallenkamp (Sanyo) was used to record the melting points of all the final products and were uncorrected. FTIR spectrophotometer (Alpha Bruker, ATR eco ZnSe, νmax in cm−1) was used to evaluate functional groups of synthesized products. 1H NMR and 13C NMR spectra were recorded using Bruker AM300 spectrophotometer in DMSO-d6 at 300 MHz and 75 MHz respectively using TMS (Tetramethyl silane) as an internal standard. LECO-183 CHN analyzer was used for elemental analysis. The progress of all reactions was monitored using thin-layer chromatography (TLC). Column chromatographic technique was used to purify the final products using silica gel HF-254 (Merck) and chloroform as eluent. All research chemicals used were of high-purity grade (99% HPLC). Rat NLRP3 Elisa kit (ab277086) was procured from Abcam, UK while Elisa COX-2 kit (Cat # 30205Ra) was purchased from Nanjing Pars Biochem CO., LTD. Rat TNF-a (Cat# E-EL-R0019) were purchased from Elabscience.
6
Synthesis and Characterization of Tyrosinase Inhibitors
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Required chemicals and solvents were procured from Sigma-Aldrich (St. Louis, MO, USA), Merck (Kenilworth, NJ, USA), and Honeywell (Charlotte, NC, USA) with no further purification before their use. Spectrophotometric and elemental analysis was used for the characterization of the synthesized derivatives. Functional group detection was performed with a Bruker ALPHA FTIR spectrometer (Billerica, MA, USA), and structural analysis was conducted with 1HNMR and 13C NMR using a Bruker AM300 spectrophotometer (Billerica, MA, USA) with DMSO as the solvent. Melting points were also recorded with the help of the Gallenkamp melting point apparatus. Mushroom tyrosinase (EC 1.14.18.1) was purchased from Sigma-Aldrich, (St. Louis, MO, USA).
7
Characterization of Organic Compounds
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All reagents were obtained from commercial sources and used without further purification, unless stated otherwise. Reaction progress was monitored by thin layer chromatography, using aluminum sheets precoated with silica gel 60 F254 (200 μm, Merck, Darmstadt, Germany). Melting points were determined on Weiss Gallenkamp melting point apparatus (Loughborough, England) and are uncorrected. IR spectra were recorded on Perkin Elmer BX-II spectrometer (Waltham, United States). 1H and 13C spectra were obtained using Bruker AM-300 spectrophotometer (Billerica, United States). Chemical shifts were described in parts per million. Mass spectra were recorded on an Applied Biosystems API 2000 mass spectrometer (Darmstadt, Germany).
8
Characterization of Novel Organic Compounds
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All chemicals were purchased from common commercial suppliers and used without further purification. Melting points (mp) were determined on a Gallenkamp melting point apparatus and were uncorrected. The IR spectra were recorded on Thermo scientific NICOLET IS10 spectrophotometer. All 1H NMR and 13C NMR spectra were recorded on Bruker AM-300 spectrophotometer at 300 and 100 MHz respectively, in DMSO as a solvent and TMS as an internal standard at Quaid-e-Azam University, Islamabad.
9
Compound Purification and Characterization
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The purification of all synthesized compounds was accomplished through recrystallization using an appropriate solvent. The purity of the compounds was subsequently confirmed via thin-layer chromatography on silica gel F254. Characterization of the synthesized compounds was conducted through spectrophotometric analyses, specifically Fourier-transform infrared spectroscopy (FTIR) using a Thermoscientific NICOLET IS10 Spectrophotometer, and proton nuclear magnetic resonance (1H NMR) using a Bruker AM-300 Spectrophotometer. DMSO and chloroform were employed as solvents in these analyses.
10
Comprehensive Spectroscopic Characterization
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High grade solvent and chemical reagents were purchased from Sigma-Aldrich. The melting points were determined using an uncorrected digital Gallenkamp (SANYO) model MPD BM 3. Using a Bruker AM-300 spectrophotometer, 1H NMR and 13C NMR spectra were acquired in CDCl3 or acetone-d6 solutions at 300 MHz and 75.4 MHz, respectively. FTIR spectra were collected using an FTS 3000 MX spectrophotometer, mass spectra (EI, 70 eV) with a GC-MS device, and elemental analyses with an LECO-183 CHNS analyzer. Chemical shifts were measured in ppm units, while coupling constants (J) were measured in hertz.
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