Oligonucleotides for site directed mutagenesis

Mitoxantrone, rhodamine-123, Ko143, ammonium molybdate, potassium antimony (III) tartrate hydrate, adenosine 5′-triphosphate disodium salt (ATP), ascorbic acid and ouabain octahydrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). PEI (Poly-ethyleneimine) is obtained from Polysciences Europe (Eppelheim, Germany). G418 is from Santa Cruz Biotechnology (Dallax, TX, USA). Sodium orthovanadate is from New England Biolabs (Frankfurt, Germany). Oligonucleotides for site-directed mutagenesis were from Eurofins (Munich, Germany). Monoclonal mouse anti-ABCG2 (BXP-21) was purchased from Santa Cruz Biotechnology (CA, USA). Rabbit anti-β-actin (D6A8) was from Cell Signaling (Danvers, MA, USA). All other chemicals were of molecular biology grade from Sigma-Aldrich (St. Louis, MO, USA). Phusion DNA polymerase and DpnI are from NEB (New England Biolabs, MA, USA). Plasmids templates using in this study, pcDNA3.1(-)-hABCG2 (wild type) and pEGFPC1-hABCG2 (wild type) were kindly provided by Balázs Sarkadi (Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary).

All chemicals were of molecular biology grade from Sigma‐Aldrich (St. Louis, MO, USA), unless stated otherwise. Mitoxantrone, rhodamine 123, pheophorbide A, Ko143, potassium antimony (III) tartrate hydrate, adenosine 5′‐triphosphate disodium salt (ATP), ammonium molybdate, ascorbic acid, and ouabain octahydrate were from Sigma‐Aldrich. Poly‐ethyleneimine was from Polysciences Europe (Eppelheim, Germany). Sodium orthovanadate was from New England Biolabs (Frankfurt, Germany). G418 was from Santa Cruz Biotechnology (Dallax, TX, USA). Oligonucleotides for site‐directed mutagenesis were purchased from Eurofins (Munich, Germany). Monoclonal mouse anti‐ABCG2 (BXP‐21) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti‐β‐actin (D6A8) was purchased from Cell Signaling (Danvers, MA, USA). Phusion DNA polymerase and DpnI are from NEB (New England Biolabs, Ipswich, MA, USA).

PLP, pyridoxine (PN), L-alanine, sodium glyoxylate, NADH, rabbit muscle L-lactic dehydrogenase (LDH), isopropyl-b-D-thiogalactopyranoside, MG132, chloroquine, and cycloheximide, were obtained from Sigma-Aldrich (St. Louis, MO, USA). The FlAsH-EDT2 probe was purchased from Thermo Scientific (Waltham, MA, USA). 8-anilino-1-naphthalenesulfonic acid (ANS) was purchased from Molecular Probes (Eugene, OR, USA). Ham’s F12 Glutamax medium without PN was purchased from Invitrogen (Carlsbad, CA, USA). Rabbit polyclonal anti-AGT human, rabbit polyclonal anti-Pro11, and guinea pig antiperoxisomal catalase antibodies were kindly provided by Prof. C.J. Danpure (University College London). Anti-rabbit, anti-guinea pig and anti-mouse horseradish peroxidase (HRP) antibodies were purchased from GE Healthcare (Chicago, IL, USA). Oligonucleotides for site-directed mutagenesis were purchased from Eurofins Genomics (Anzinger, Germany). All other chemicals were of the highest grade commercially available.