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Aotf controlled laser launch

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The AOTF-controlled laser launch is a specialized piece of lab equipment designed to provide precise control over the output of a laser. It utilizes an Acousto-Optic Tunable Filter (AOTF) to modulate the intensity, wavelength, and direction of the laser beam. This allows for accurate and dynamic control over the laser's properties, making it a versatile tool for various experimental and research applications.

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Lab products found in correlation

Spinning disc scan head, by Yokogawa (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Ix71 microscope, by Yokogawa (2 mentions) Mef 2 nucleofector kit, by Lonza (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

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AOTF-controlled lasers (2 citations)

3 protocols using aotf controlled laser launch

1

Live Cell Imaging of Transfected HEK293 Cells

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HEK293 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum. Cells were plated on 35-mm glass bottom imaging dishes and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. Sixteen to twenty-four hours after transfection, cells were moved to Hepes-buffered Saline (HBS) with 1 mM CaCl2 for imaging. Live cell imaging was performed at 33.5 °C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a × 60/NA 1.4 objective. Excitation illumination was delivered from an AOTF-controlled laser launch (Andor Technology) and images were collected on a 1,024 × 1,024 pixel EM-CCD camera (iXon; Andor Technology).
Taslimi A., Zoltowski B., Miranda J.G., Pathak G., Hughes R.M, & Tucker C.L. (2016). Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature chemical biology, 12(6), 425-430.
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2

Peroxisomal Localization Assay with EGFP-SKL

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For peroxisomal localization studies, a ‘LQSKL’ PTS1 signal sequence from acyl-CoA oxidase was appended to the C-terminus of an EGFP reporter construct (in pCDNA3.1) using PCR. MEFs from wild-type or homozygous mutant Pex10CY embryos were plated on 18-mm glass coverslips in DMEM/F12 (Gibco) with 10% FBS, and transiently transfected with the EGFP-SKL reporter using MEF 2 Nucleofector Kit (Lonza) according to the manufacturer’s protocol. 24 hours after transfection, cells were fixed using 4% paraformaldehyde and imaged on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a 60×/NA 1.4 objective. 488-nm excitation illumination was delivered from an AOTF controlled laser launch (Andor Technology) and images collected on a 1024 × 1024 pixel EM-CCD camera (iXon; Andor Technology).
Hanson M.G., Fregoso V., Vrana J.D., Tucker C.L, & Niswander L.A. (2014). Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. Developmental biology, 395(1), 84-95.
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3

Live Cell Imaging of Transfected HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum. Cells were plated on 35-mm glass bottom imaging dishes and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. Sixteen to twenty-four hours after transfection, cells were moved to Hepes-buffered Saline (HBS) with 1 mM CaCl2 for imaging. Live cell imaging was performed at 33.5 °C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a × 60/NA 1.4 objective. Excitation illumination was delivered from an AOTF-controlled laser launch (Andor Technology) and images were collected on a 1,024 × 1,024 pixel EM-CCD camera (iXon; Andor Technology).
Taslimi A., Zoltowski B., Miranda J.G., Pathak G., Hughes R.M, & Tucker C.L. (2016). Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature chemical biology, 12(6), 425-430.
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