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Rad18 is a protein that plays a role in DNA damage repair processes. It functions as an E3 ubiquitin-protein ligase, which catalyzes the attachment of ubiquitin molecules to target proteins. Rad18 is involved in the regulation of DNA damage tolerance pathways.

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Lab products found in correlation

Flag tag (flag), by Merck Group (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Histone h2a x, by Cell Signaling Technology (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Hsp90, by Merck Group (1 mentions) Phospho p53, by Cell Signaling Technology (1 mentions) Nb100 56506, by Novus Biologicals (1 mentions) 6xhis, by Cell Signaling Technology (1 mentions) Rpa32, by Cell Signaling Technology (1 mentions) Alexa fluor 594 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Flag m2, by Merck Group (1 mentions) γh2ax, by Merck Group (1 mentions) Patm s1981, by Abcam (1 mentions) β tubulin, by Abcam (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Hrp linked anti rabbit igg, by Cell Signaling Technology (1 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions) Rap80, by Fortis Life Sciences (1 mentions) Brca1, by Santa Cruz Biotechnology (1 mentions) P chk1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Rabbit anti-RAD18 (2 citations)

2 protocols using rad18

1

Investigating hnRNP E1 Knockdown in Cell Lines

Cited 3 times
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Human lung epithelial cell line A549 (ATCC), its hnRNP E1 knockdown (A549/E1KD or E1KD), normal mouse mammary epithelial (NMuMG) cell line, and its hnRNP E1 knockdown (NMuMG/E1KD or E1KD) derivative cells (Hussey et al, 2011 (link), 2012 (link)) were cultured in DMEM supplemented with 5% FBS and 5% FCS at 37°C in a 5% CO2 humidified chamber. Antibodies used in the work were against the following proteins: hnRNP E1 (mouse; Abnova; mouse, MBL; and rabbit, raised against purified hnRNP E1), TRF2 (rabbit, NB10056506; Novus), Hsp90 (mouse; SCBT), FLAG (rabbit; Sigma-Aldrich), 6XHis (rabbit; Cell signaling), phospho-ATM (MAB 2401; Abnova), ATM (2873S; Cell signaling), phospho-ATR, ATR (13934S; Cell signaling), phospho-p53 (9284S; Cell signaling), p53 (2524; Cell signaling), γ-H2A.X (rabbit, 9718S; Cell signaling), Histone H2A.X (7631T; Cell signaling), PCNA (mouse, Cat no. 2586S; SCBT), Usp1 (rabbit, 8033S; Cell signaling), Usp7 (mouse, HAUSP; SCBT), RPA32 (rat, 2208S; Cell signaling), Rad6 (R6A/R6B, rabbit, 4944S; Cell signaling), Rad18 (rabbit, 9040S; Cell signaling), and Fen1 (rabbit; SCBT). Plasmids containing hnRNP E1 and its three KH domains namely KH1, KH2, and KH3 have been described previously (Chaudhury et al, 2010b (link); Brown et al, 2016 (link)); each of the four ORFs contained an N-terminal GST tag.
Mohanty B.K., Karam J.A., Howley B.V., Dalton A.C., Grelet S., Dincman T., Streitfeld W.S., Yoon J.H., Balakrishnan L., Chazin W.J., Long D.T, & Howe P.H. (2021). Heterogeneous nuclear ribonucleoprotein E1 binds polycytosine DNA and monitors genome integrity. Life Science Alliance, 4(9), e202000995.
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2

Antibody Panel for DNA Damage Response

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Primary antibodies used in this study were Flag M2 (Sigma, F1804), Myc (Santa Cruz Biotechnology, SC-40), H2AX (Millipore, 07-627), γH2AX (Millipore, 05-636), H2AZ (Cell Signaling, 2718S), macroH2A (Abcam, AB37264), H2A.Bbd (Millipore, 06-1319), ZMYM3 (Abcam, AB106626), ATM (Santa Cruz Biotechnology, SC-135663), pATM S1981 (Abcam, AB81292), β-tubulin (Abcam, AB6046), RAD51 (Abcam, AB88572), RAP80 (Bethyl Laboratories, A300-763A), ABRA1 (Abcam, AB139191), BRCA1 (Santa Cruz Biotechnology, SC-6954), HRP-linked anti-GST (Sigma, A7340), MBP (Abcam, AB9084), phospho-H3 S10 (Cell Signaling, 3377), 53BP1 (Novus Biologicals, NB100-304), RAD18 (Cell Signaling, 9040), RPA2 (Abcam, AB2175), pRPA2 S33 (Bethyl Laboratories, A300-246), pRPA2 S4/S8 (Bethyl Laboratories, A300-245), Chk1 (Santa Cruz Biotechnology, SC-8408), and pChk1 (Cell Signaling, 2348). For Western blotting analysis, secondary antibodies HRP-linked anti-rabbit IgG and HRP-linked anti-mouse IgG were purchased from Cell Signaling. For immunofluorescence, Alexa fluor 488 goat anti-rabbit and Alexa fluor 594 goat anti-mouse were used (Invitrogen).
Leung J.W., Makharashvili N., Agarwal P., Chiu L.Y., Pourpre R., Cammarata M.B., Cannon J.R., Sherker A., Durocher D., Brodbelt J.S., Paull T.T, & Miller K.M. (2017). ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes & Development, 31(3), 260-274.
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