Buprenorphine

Manufactured by LGC

Sourced in United Kingdom

Buprenorphine is a laboratory equipment used for the analysis and detection of the controlled substance buprenorphine. It is a powerful analytical tool that can be utilized in various research and testing applications.

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Lab products found in correlation

3 protocols using buprenorphine

Quantification of Buprenorphine in Rabbit Plasma

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Analytical grade buprenorphine was purchased from LGC Standards (Teddington, UK) and buprenorphine-D4 was purchased from Sigma-Aldrich (Darmstadt, Germany). Ethyl acetate (POCH, Gliwice, Poland), n-hexane (Merk, Darmstadt, Germany) and ammonia solution 25% (Stanlab, Lublin, Poland) were of HPLC or analytical grade. Formic acid and methanol were from Sigma-Aldrich (Darmstadt, Germany) and were of analytical grade and LC-MS grade, respectively.
The plasma samples used in the method development and validation were obtained from rabbits involved in the pharmacokinetic/pharmacodynamic study. All blank plasma samples were obtained from animals which did not receive any drugs except isoflurane used in inhalatory anesthesia.

Tikhomirov M., Poźniak B, & Śniegocki T. (2021). High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Buprenorphine Evaluation in Plasma—Application to Pharmacokinetic Studies in Rabbits. Molecules, 26(2), 437.

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Opioid Receptor Ligand Characterization

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U-47700 and furanyl fentanyl were procured from Cayman Chemical. Hydromorphone hydrochloride was obtained from Fagron. Buprenorphine, norBuprenorphine, fentanyl, loperamide, carfentanil, and naloxone were obtained from LGC Standards. Dulbecco's modified Eagle's medium, Opti-MEM I Reduced Serum Medium, penicillin and streptomycin (5000 IU/mL and 5000 g/mL), amphotericin B (250 g/mL), glutamine (200 mmol/L), the restriction enzyme SacI, and the DNA polymerase (Phusion polymerase) were purchased from Thermo Fisher Scientific. Primers were procured from Eurofins Genomics. [d-Ala 2 -MePhe 4 -Gly-ol]encephalin (DAMGO), fetal bovine serum (FBS), and poly-D-lysine were from Sigma Aldrich. Detailed information on chemical reagents used for processing of blood samples can be found elsewhere (13 ) .

Cannaert A., Vasudevan L., Friscia M., Mohr A.L.A., Wille S.M.R, & Stove C.P. (2018). Activity-Based Concept to Screen Biological Matrices for Opiates and (Synthetic) Opioids. Clinical chemistry, 64(8).

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Analytical Standards for Pharmaceutical Compounds

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Standards for acetaminophen (ACM), atenolol (ATE), bezafibrate (BZB), carbamazepine (CBZ), codeine (COD), diclofenac (DCF), ibuprofen (IBP), ketoprofen (KET), metoprolol (MET), oxazepam (OXA), salicylic acid (SCA), sulfamethoxazole (SUL), tramadol (TRA) and trimethoprim (TMP) were purchased from Sigma-Aldrich with a purity > 98%. The standards for 6-mono-acetylmorphine (6MAM), amphetamine (AMP), benzoylecgonine (BZE), buprenorphine (BUP), cocathylene (CET), cocaine (COC), heroin (HER), 3,4-methylene-dioxy-Nmethylamphetamine (MDMA), methadone (METD), morphine (MOR) and 11-nor-delta-9hydroxytetrahydrocannabinol (THC-COOH) were purchased from LGC Standards. Extraction and separation solvents, methanol (MeOH) and acetonitrile (AcN) were purchased from Fisher-Scientific, assuming an analytical grade (purity up to 99.95 %).

Thiebault T., Fougère L., Destandau E., Réty M, & Jacob J. (2017). Temporal dynamics of human-excreted pollutants in wastewater treatment plant influents: Toward a better knowledge of mass load fluctuations. The Science of the total environment, 596-597.

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