Protein extracts were obtained using cell lysis buffer (20 mM HEPES, 400 nM NaCl, 0.5% NP-40, 10% glycerol, 1 mM DTT) with protease inhibitor (Roche) and phosphatase inhibitor. Proteins were separated by SDS-PAGE, transferred to polyvinylidene fluoride membrane (Bio-Rad), and probed with specific primary antibody and appropriate HRP-conjugated secondary antibody. Signals were detected using SuperSignal West Pico Chemiluminescent Substrate (Pierce). The following antibodies were used for western blot: α-STAT3 (1:1,000, Santa Cruz, sc-482), p-STAT3Y705 (1:1,000, Cell Signaling Technology, no. 9131), METTL8 polyclonal (1:2,000, produced by our lab), METTL8 monoclonal (1:1,000, produced by our lab), α-tubulin (1:2,500, Santa Cruz, sc-53646), β-actin (1:2,500, Santa Cruz, sc-8432), SOCS3 (1:1,000, Cell Signaling, no. 2923), OCT4 (1:1,000, Cell Signaling, no. 2840), SOX2 (1:200, Santa Cruz, sc-365964), MAPKBP1 (1:200, Santa Cruz, sc-514754), and Flag (1:1,000, Sigma, F1804).
α stat3
Manufactured by Santa Cruz Biotechnology
α-STAT3 is a primary antibody that recognizes the phosphorylated form of STAT3 (Signal Transducer and Activator of Transcription 3) protein. STAT3 is a transcription factor that plays a crucial role in various cellular processes such as cell growth, differentiation, and apoptosis. The α-STAT3 antibody is a valuable tool for researchers studying the activation and regulation of the STAT3 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
α gapdh, by Merck Group (2 mentions)
Socs3, by Cell Signaling Technology (1 mentions)
P stat3 y705, by Cell Signaling Technology (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
Protease inhibitor, by Roche (1 mentions)
α β actin, by Merck Group (1 mentions)
α p p65, by Cell Signaling Technology (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions)
Cyanogen bromide activated sepharose 4b, by Merck Group (1 mentions)
Cellrox green reagent, by Thermo Fisher Scientific (1 mentions)
α actin, by MP Biomedicals (1 mentions)
α egfr, by Santa Cruz Biotechnology (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Dako fluorescent mounting medium, by Agilent Technologies (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
3 protocols using α stat3
1
Western Blot Analysis of Protein Extracts
2
Western Blot Analysis of Placental Proteins
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Human and mouse placental tissues and human cultured trophoblast cells were lysed in Ripa buffer (50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Na‐D.O.C., 0.1% SDS, 1% Triton X‐100) containing protease inhibitors (Sigma), using a tissue homogenizer. Equal amounts of protein were separated on 12% SDS–polyacrylamide gels and transferred to PVDF membranes. Detection was performed with the following primary antibodies: α‐p21 (BD Biosciences), α‐p53, α‐Stat3, α‐p65 (Santa Cruz Biotechnology), α‐p16 (Abcam), α‐DCR2 (Enzo Life Sciences), α‐Smad3,α‐p‐Smad3, α‐p‐Stat3, α‐p‐p65, α‐Mek1/2, α‐p‐Mek1/2 (Cell Signaling), α‐β‐actin (Sigma), and α‐GAPDH (Merck). The detailed list of antibodies is presented in Appendix Table S5 .
3
Epidermal Growth Factor Receptor Signaling
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Dulbecco’s modified Eagle’s media (DMEM), Fetal bovine serum (FBS), Penicillin-Streptomycin, Trypsin-EDTA, Epidermal growth factor (EGF) (Peprotech), Piperonylic acid, Tyrphostin AG1478, Cyanogen bromide-activated-Sepharose® 4B, Thiazolyl Blue Tetrazolium Bromide (MTT), Hoechst 33342 (Sigma-Aldrich), G418 (Calbiochem), Cell counting kit-8 (CCK-8) (Dojindo), Dako fluorescent mounting medium (Dako), CellROX® Green Reagent (Life technologies), α-p-ERK T202/Y204, α-p-AKT S473, α-p-PLCγ1 Y783, α-ERK, α-AKT, α-PLCγ1, α-Egr-1 (CST), α-p-Tyr, α-EGFR, α-c-Fos, α-p-STAT3 S727, α-STAT3 (SantaCruz), α-FGFR2 (Abcam), α-GAPDH (Millipore), α-Actin (MP biomedicals), α-mouse IgG HRP conjugate (Thermo), and α-rabbit IgG HRP conjugate (Promega).
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