Sections of cartilage nodule tissue were deparaffinized and rehydrated and then treated with 3% H2O2. Slides were next incubated in 0.01 M citrate buffer for 20 min at 94–98°C and blocked with 5% BSA. Primary antibodies included rabbit anti-human collagen type II antibodies (1:80, Sigma-Aldrich). The staining was visualized using a microscope (Axioskop 40, Zeiss) by applying streptavidin-biotin complex reagent (Boster) and 3,3′-diaminobenzidine (DAB; Boster) after the treatment with a solution of biotinylated goat anti-rabbit IgG (Boster). Sections were treated using the same process but without incubation with a primary antibody as the negative control.
Rabbit anti human collagen type 2 antibodies
Manufactured by Merck Group
Sourced in China
Rabbit anti-human collagen type II antibodies are laboratory reagents used in research applications. These antibodies are specifically raised against human collagen type II, a major structural component of cartilage. They can be used to detect and study collagen type II in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin biotin complex reagent, by Boster Bio (1 mentions)
3 3 diaminobenzidine dab, by Boster Bio (1 mentions)
Biotinylated goat anti rabbit igg, by Boster Bio (1 mentions)
Axioskop 40, by Zeiss (1 mentions)
Dexamethasone (dex), by MP Biomedicals (1 mentions)
Proline, by Merck Group (1 mentions)
H dmem, by Thermo Fisher Scientific (1 mentions)
Transforming growth factor β1, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 protocols using rabbit anti human collagen type 2 antibodies
1
Immunohistochemical Analysis of Cartilage Tissue
2
Chondrogenic Differentiation of SFMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SFMSCs were harvested using 0.25% trypsin and approximately 3×105 cells were transferred to a 15 ml centrifuge tube. They were then centrifuged at 450 × g for 8 min, and 500 μl of chondrocyte differentiation induction medium [H-DMEM, 1X ITS-A (both from Gibco), 100 nM dexamethasone (MP Biomedicals), 50 mM ascorbic acid, 40 mg/ml proline (both from Sigma-Aldrich), and 10 ng/ml transforming growth factor-β1 (PeproTech, Rocky Hill, NJ, USA)] were added. The medium was refreshed every 3 days. Following culture for 28 days, the chondrogenic pellets were fixed with 4% formalin and paraffin-embedded. Chondrogenic differentiation was assessed by 0.1% Safranin O staining (Cat. no. S2255; Seebio, Shanghai, China) and immunohistochemical staining for collagen II [rabbit anti-human collagen type II antibodies (Sigma-Aldrich) 1:80]. The control group was then incubated with complete culture medium.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!