Anti slc3a2

50 mg of xenograft tissues were homogenized and lysed using RIPA lysis buffer containing protease inhibitor cocktails (Thermo Scientific). Protein was quantified using the BCA assay, and an equal amount of protein for each lysate (50 ug) was resolved by 4–12% gradient SDS-PAGE followed by transfer onto nitrocellulose membranes. Membranes were blocked with 5% milk for 1 hour in tris buffered saline and probed with primary antibodies for overnight at 4° C in tris buffered saline. Washing was followed by incubation with secondary antibody for 1 hour at room temperature in tris buffered saline containing 0.1% Tween-20. Anti-GPX4 (sc-166120, 1:1000 for WB) and anti-GAPDH antibodies (sc-32233, 1:3000) were obtained from Santa Cruz Biotechnology. Anti-SLC7A11 antibody was purchased from Invitrogen, Thermo Fisher Scientific (PA1-16775, 1:500 for WB). Anti-SLC3A2 was purchased from Santa Cruz (sc-376815). Secondary antibodies with HRP were obtained from Fisher Scientific (anti-mouse PI31432, and anti-rabbit PI31462, 1:2000). Pierce^™ ECL Western Blotting Substrate (Thermo Fisher Scientific, PI32106) was used to develop chemiluminescent signals that were measured on an IVIS Lumina imager.