Mice were given a 4 ml/kg intraperitoneal injection of DHF (5 mg/kg) dissolved in the vehicle (18 % DMSO in PBS solution) 2 h prior to noise trauma. For in vitro treatments, two protocols were used for the treatment of cochlear explants: 1) DHF day-night experiments (Figure 3D–F ): Cochlear explants were exposed to DMSO vehicle or DHF 60 μM either at ZT 3 or ZT12. Dexamethasone 21 phosphate disodium salt (Sigma Aldrich, D1159) was applied on day 4. 2) DHF/ANA12 experiments (Figure 3G–I ): Cochlear explants were pre-exposed on day 0 and day 2 with DMSO vehicle or ANA12 (Sigma Aldrich, SML0209) at ZT 4. On day 3 the explants were co-treated with DHF (Tocris, 3826) and vehicle or ANA12 at ZT 4.
Sml0209
Manufactured by Merck Group
The SML0209 is a lab equipment product manufactured by Merck Group. It is a precision instrument designed for scientific and research applications. The core function of the SML0209 is to perform accurate measurements and analysis. Further details about its intended use or specific applications are not available.
Automatically generated - may contain errors
2 protocols using sml0209
1
Investigating DHF-Mediated Otoprotection
2
Modulation of Neurotrophin Signaling
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Transfected cells were treated 90 min with hBDNF (10 ng/ml, Alomone Labs #B-250), NT-3 (10 ng/ml, Alomone Labs #N-260), or NT-4 (10 ng/ml, Alomone Labs #N-270) and/or rh TrKB-Fc (1 μg/ml, R&D Systems #688-TK-100). Otherwise, the cells were treated overnight with ERK 1/2 inhibitor: PD98059 (25 μM/ml, Calbiochem#513000) or GSK3β inhibitor: GSK3βIX (5 μM/ml, Calbiochem #328007) or DMSO (equal volume; Sigma D2438) & pharmacological inhibitor (ANA-12, 400 nM, Sigma-Aldrich #SML0209).
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