The epidemiological survey was carried out using internationally standardized methods and following a common protocol19 (link). Information on demographics, socioeconomic status, and lifestyle factors was collected using standardized questionnaires. The methods of measuring blood pressure, height, weight and waist circumference parameters were based on previous studies19 (link). Fasting venous blood samples were taken and the levels of serum TC, TG, HDL-C, and LDL-C in the samples were directly determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co. Antrim, United Kingdom, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan); respectively. Serum ApoA1 and ApoB levels were assessed by the immunoturbidimetric assay using a commercial kit (RANDOX Laboratories Ltd.)19 (link)20 (link). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University. The normal values of serum TC, TG, HDL-C, LDL-C, ApoA1 and ApoB levels and the ratio of ApoA1 to ApoB in our Clinical Science Experiment Center were 3.10–5.17, 0.56–1.70, 1.16–1.42, 2.70–3.10 mmol/L, 1.20–1.60, 0.80–1.05 g/L and 1.00–2.50, respectively21 (link)22 (link).
Type 7170a
Manufactured by Hitachi
Sourced in Japan, France
The Type 7170A is a laboratory equipment product from Hitachi. It is designed for precise measurement and analysis tasks in research and industrial settings. The core function of the Type 7170A is to provide accurate and reliable data acquisition and processing capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Tcho 1, by Randox (6 mentions)
Tg lh, by Randox (6 mentions)
Immunoturbidimetric immunoassay, by Randox (5 mentions)
Icam 1, by RayBiotech (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Trisodium citrate, by Sangon (1 mentions)
Citric acid, by Sangon (1 mentions)
Immunoturbidimetric assay, by Randox (1 mentions)
40 protocols using type 7170a
1
Epidemiological Survey of Lipid Profiles
2
Dyslipidemia Risk Factors Assessment
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At the time of enrollment, background data, such as gender, age, height, and body weight, were collected from each subject.
The risk factors of dyslipidemia, including smoking, drinking status, and exercise habit, were collected with a formatted questionnaire. The primary variables of lifestyle were viewed as the following. (1) Current smoking was defined as smoking at least one cigarette a day for more than one year; (2) current drinking was defined as drinking at least 500 mL of beer or 150 mL of wine or distilled spirits a day for more than one year; (3) average weekly exercise less than three times was defined as “no exercise” and average weekly exercise three or more times was defined as “regular exercise”.
Venous blood was collected after fasting for eight hours, and the concentration of the TG, TC, LDL-C, and HDL-C were analyzed by enzymatic methods with a commercially available kit (RANDOX Laboratories Ltd., London, England). Each measurement was performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan).
The risk factors of dyslipidemia, including smoking, drinking status, and exercise habit, were collected with a formatted questionnaire. The primary variables of lifestyle were viewed as the following. (1) Current smoking was defined as smoking at least one cigarette a day for more than one year; (2) current drinking was defined as drinking at least 500 mL of beer or 150 mL of wine or distilled spirits a day for more than one year; (3) average weekly exercise less than three times was defined as “no exercise” and average weekly exercise three or more times was defined as “regular exercise”.
Venous blood was collected after fasting for eight hours, and the concentration of the TG, TC, LDL-C, and HDL-C were analyzed by enzymatic methods with a commercially available kit (RANDOX Laboratories Ltd., London, England). Each measurement was performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan).
3
Lipid Profile and Apolipoprotein Measurement
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A fasting venous blood sample of 5 ml was drawn from the participants. A part of the sample (2 mL) was collected into glass tubes and used to determine serum lipid levels. Another part of the sample (3 mL) was transferred to tubes with anticoagulants (4.80 g/L citric acid, 14.70 g/L glucose and 13.20 g/L tri-sodium citrate) and used to extract deoxyribonucleic acid (DNA). Measurements of serum TC, TG, HDL-C, and LDL-C levels in the samples were performed by enzymatic methods with commercially available kits (RANDOX Laboratories Ltd., Ardmore, Diamond Road, CrumlinCo. Antrim, United Kingdom, BT29 4QY; Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan). Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay using a commercial kit (RANDOX Laboratories Ltd.). All determinations were performed with an auto-analyzer (Type 7170 A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University52 (link), 53 (link).
4
SNP Genotyping of XKR6 Gene
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A 5-mL venous blood sample was collected from each participant after at least 12
hours of fasting. Part of the sample (2 mL) was placed in a glass tube and used
to perform biochemical assays. Another part of the sample (3 mL) was collected
in tubes containing anticoagulants (4.80 g/L citric acid, 14.70 g/L glucose,
13.20 g/L trisodium citrate) and was utilized to extract DNA. Genotyping of the
XKR6 rs7014968 SNP was performed by Snapshot technology in
the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co Ltd, China.23 (link),26 (link)–38 (link, link, link, no link found, no link found, link, link, link, link, link, link, link)
5′-TGGAACTAATCGTTGTTGCCAGTC-3′ and 5′-TCCCAGTGAAAAGCAAACCAGAA-3′, respectively.
Seven serum lipid traits (TC, TGs, HDL-C, LDL-C, ApoA1, ApoB, and ApoA1/ApoB)
were determined as described in our previous reports.23 (link),26 (link)–38 (link, link, link, no link found, no link found, link, link, link, link, link, link, link)
Hitachi Ltd, Tokyo, Japan) in the Clinical Science Experiment Center of The
First Affiliated Hospital, Guangxi Medical University.
hours of fasting. Part of the sample (2 mL) was placed in a glass tube and used
to perform biochemical assays. Another part of the sample (3 mL) was collected
in tubes containing anticoagulants (4.80 g/L citric acid, 14.70 g/L glucose,
13.20 g/L trisodium citrate) and was utilized to extract DNA. Genotyping of the
XKR6 rs7014968 SNP was performed by Snapshot technology in
the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co Ltd, China.23 (link),26 (link)
5′-TGGAACTAATCGTTGTTGCCAGTC-3′ and 5′-TCCCAGTGAAAAGCAAACCAGAA-3′, respectively.
Seven serum lipid traits (TC, TGs, HDL-C, LDL-C, ApoA1, ApoB, and ApoA1/ApoB)
were determined as described in our previous reports.23 (link),26 (link)
Hitachi Ltd, Tokyo, Japan) in the Clinical Science Experiment Center of The
First Affiliated Hospital, Guangxi Medical University.
5
Lipid Profile of Blood Samples
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A fasting venous blood sample of 5 ml was obtained from each participant. A part sample of 2 ml was placed into a glass tube to perform biochemical assays, whereas another part sample of 3 ml was collected into an anticoagulant tube to extract deoxyribonucleic acid (DNA). The levels of serum HDL-C (Cholestest N HDL), low-density lipoprotein cholesterol (LDL-C, Cholestest LDL; Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), total cholesterol (TC, Tcho-1), TG (TG-LH), apolipoprotein (Apo) A1 and ApoB (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co. Antrim, United Kingdom, BT29 4QY) in samples were determined using an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in our Clinical Science Experiment Center (47 (link)).
6
Serum Lipid and DNA Extraction Protocol
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Fasting venous blood samples of 5 ml were collected from each subject. A portion of the sample (2 ml) was placed in a tube and used to measure serum lipid levels. The remaining sample of 3 ml was collected in a glass tube containing anticoagulants (14.70 g/L glucose, 13.20 g/L trisodium citrate, 4.80 g/L citric acid) and utilized to extract deoxyribonucleic acid (DNA). The methods for performing serum ApoA1, HDL-C, ApoB, TG, LDL-C and TC measurements were described in a previous study29 (link). All determinations were conducted using an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University30 (link),31 (link).
7
Serum Lipid and Apolipoprotein Profiling
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Venous blood samples were obtained from all subjects after at least 12 h of fasting. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in samples were determined by enzymatic methods with commercially available kits, Tcho-1, TG-LH (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co., Antrim, UK, BT29 4QY), Cholestest N HDL, and Cholestest LDL (Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan), respectively. Serum apolipoprotein (Apo) A1 and ApoB levels were detected by the immunoturbidimetric immunoassay (RANDOX Laboratories Ltd.). All determinations were performed with an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [22 (link),23 (link),24 (link),25 (link),26 (link),27 (link),28 (link),29 (link),30 (link),31 (link),32 (link),33 ].
8
Blood Sample Collection and Analysis for Lipid Profiles
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A fasting venous blood sample of 5 ml was collected from each participant. A portion of the sample (2 ml) was collected in a test tube and used to measure serum lipid levels. The rest of the sample (3 ml) was collected into a test tube containing anticoagulants (13.20 g/l tri-sodium citrate, 14.70 g/l glucose, and 4.80 g/l citric acid) and utilized to extract deoxyribonucleic acid (DNA). Methods for measuring serum LDL-C, apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), TC, TG, and HDL-C were described in detail in a previous study (Sun et al., 2015 (link)). All measurements were performed using an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University (Guo et al., 2015b (link)).
9
Venous Blood Sampling for Lipid and DNA Analysis
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Venous blood samples of 5 mL were drawn after at least 12 h of fasting. Two-fifths of the sample (2 mL) was collected in glass tubes and used to determine serum lipid levels. The remaining three-fifths of the sample (3 mL) was transferred to tubes with anticoagulants (4.80 g/l citric acid, 14.70 g/l glucose and 13.20 g/l trisodium citrate) and used to extract DNA. Measurements of serum TC, TAG, HDL-C, and LDL-C levels in the samples were performed by enzymatic methods with commercially available kits (RANDOX Laboratories Ltd., BT29 4QY; Daiichi Pure Chemicals Co., Ltd.). Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay using a commercial kit (RANDOX Laboratories Ltd.). All determinations were performed with an auto-analyser (Type 7170A; Hitachi Ltd.) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [15 (link)–17 (link)].
10
Measurement of Serum Lipid Profiles
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A fasting venous blood sample of 5 ml was drawn from the participants. A part of the sample (2 mL) was collected into glass tubes and used to determine serum lipid levels. Another part of the sample (3 mL) was transferred to tubes with anticoagulants (4.80 g/L citric acid, 14.70 g/L glucose and 13.20 g/L tri-sodium citrate) and used to extract deoxyribonucleic acid (DNA). Measurements of serum TC, TG, HDL-C, and LDL-C levels in the samples were performed by enzymatic methods with commercially available kits (RANDOX Laboratories Ltd., Ardmore, Diamond Road, Crumlin Co. Antrim, United Kingdom, BT29 4QY; Daiichi Pure Chemicals Co., Ltd., Tokyo, Japan). Serum ApoA1 and ApoB levels were detected by the immunoturbidimetric immunoassay using a commercial kit (RANDOX Laboratories Ltd.). All determinations were performed with an auto-analyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University [55 (link), 56 (link)].
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