Experiments were conducted in 12 operant experimental chambers (Model ENV-008CT, Med Associates, Inc., St. Albans, VT, USA) located within ventilated sound attenuating enclosures (ENV-018MD) located in a designated room. Each chamber featured, on one wall, two amber stimulus lights (Model ENV-221M) located directly above two retractable levers (Model ENV-112CM) positioned 2.1 cm above the stainless steel grid floor and 7.62 cm apart from one another, a center receptacle located between the two levers, and a pellet feeder (Model ENV-200R2M). A house light and ventilator fans were located on the opposite wall. Experimental contingencies and data collection were controlled using a computer-driven interface (Model SG-503, MED Associates, St. Albans, VT, USA).
Env 018md
Manufactured by Med Associates
Sourced in United States
The ENV-018MD is a laboratory instrument designed for environmental monitoring. It is capable of measuring various environmental parameters, such as temperature, humidity, and atmospheric pressure. The device is intended for use in controlled laboratory settings to support environmental research and analysis activities.
Automatically generated - may contain errors
Lab products found in correlation
Env 007ct, by Med Associates (4 mentions)
Env 414, by Med Associates (3 mentions)
Env 005, by Med Associates (3 mentions)
Ethovision xt, by Noldus (2 mentions)
Model env 008ct, by Med Associates (1 mentions)
Plexiglass operant chambers, by Med Associates (1 mentions)
Env 008, by Med Associates (1 mentions)
Clocklab data acquisition system, by Actimetrics (1 mentions)
Med pc for windows software, by Med Associates (1 mentions)
Med pc for windows version 4, by Med Associates (1 mentions)
Env 008ct, by Med Associates (1 mentions)
10 protocols using env 018md
1
Operant Conditioning Experimental Setup
2
Operant Conditioning Chambers for Behavioral Studies
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12 plexiglass operant chambers (11′L × 8 ¼ ′W × 13′H), custom-built by Med Associates Inc (St. Albans, VT) were contained in sound-attenuating cubicles (ENV-018MD) each with an exhaust fan that helped mask external noise. Each chamber was equipped with one photo-beam lickometer reward port (CT-ENV-251L-P) located in the center of the front panel, with its sipper tube 7.5 cm above the grid floor. Two infrared (IR) sensors were positioned to detect reward port entry and sipper tube licking, respectively. Water reward was delivered through a custom-built multi-barrel sipper tube. The delivery system was controlled by pressurized air (2.6 psi) and each solenoid opening (10 msec) was calibrated to deliver a 10 μl drop of water. The reward port was flanked by two nosepoke ports (ENV-114M), located 6.6 cm to each side and 5.9 cm above the grid floor. The nosepoke ports were inactive in the behavioral tasks.
Each chamber was equipped with two ceiling-mounted speakers (ENV-224BM) to deliver auditory stimuli controlled by an audio generator (ANL-926), and two stimulus lights (ENV-221) positioned above the reward port in the front panel. Behavior training protocols were controlled by Med-PC software (Version IV), which stored all event timestamps at 2 msec resolution and also sent out TTL signals to neurophysiology recording systems to register event timestamps.
Each chamber was equipped with two ceiling-mounted speakers (ENV-224BM) to deliver auditory stimuli controlled by an audio generator (ANL-926), and two stimulus lights (ENV-221) positioned above the reward port in the front panel. Behavior training protocols were controlled by Med-PC software (Version IV), which stored all event timestamps at 2 msec resolution and also sent out TTL signals to neurophysiology recording systems to register event timestamps.
3
Threat Conditioning Behavioral Assay
4
Threat Conditioning Behavioral Assay
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A threat conditioning chamber (26 × 30 × 33 cm, ENV-007CT, MED Associates) with a metal grid floor (ENV-005, MED Associates) connected to a standalone aversive electric shock stimulator (ENV-414S, MED Associates) was used for foot shock delivery. A USB camera (DFK 33GX236, Imagine Source) was connected to a computer, and video tracking software (Ethovision XT, Noldus) was used for shock delivery and behavioral analysis. The chamber was enclosed in a light- and sound-attenuating cubicle (ENV-018MD, MED Associates). The chamber was cleaned with 70% ethanol and double-distilled water after each trial.
For both Miniscope and loss-of-function experiments, mice were placed inside the chamber without habituation. After a 2 min baseline, an electric shock (2 sec, 0.6 mA) was delivered, and behavior was recorded for 2 more min. For loss-of-function experiments, freezing behavior was monitored one day before (habituation), immediately after (conditioning), and one day after the shock (post-test). For the terminal inhibition experiment, procedures were the same as for the whole-population inhibition experiment, except that the laser was turned on throughout the conditioning and post-test periods.
For both Miniscope and loss-of-function experiments, mice were placed inside the chamber without habituation. After a 2 min baseline, an electric shock (2 sec, 0.6 mA) was delivered, and behavior was recorded for 2 more min. For loss-of-function experiments, freezing behavior was monitored one day before (habituation), immediately after (conditioning), and one day after the shock (post-test). For the terminal inhibition experiment, procedures were the same as for the whole-population inhibition experiment, except that the laser was turned on throughout the conditioning and post-test periods.
5
Fear Conditioning Behavioral Assessment
6
Rodent Operant Chamber Setup
7
Operant Conditioning Chamber Setup and Procedure
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Four operant conditioning chambers (MED Associates, Inc., Model ENV-018MD). The boxes were 31 cm high, 24 cm long, and 31 cm wide. The front panel was divided into three columns. A 5.5 × 6 cm feeder opening, located 3 cm above the grid floor, gave access to the food when the hopper was activated and illuminated by a 2.8 w light. Over the feeder, placed 22 cm above the grid floor, there was a 2.54 cm diameter white cue key. The side columns were equipped with 2.54 cm diameter keys, also placed 22 cm above the grid floor. The three keys were 9 cm apart, center to center. The side keys could be illuminated in different colors. The 2.8 w house light was centrally located in the rear panel of the chamber, 27 cm above the grid floor. Each cage was located inside an isolated sound chamber (ENV-018V), equipped with a fan that circulated air and masked extraneous noises.
The experimental procedure was approved by the local Ethical Committee of the Center for Studies and Investigations in Behavior, by the University of Guadalajara committee for animal experiments, and met governmental guidelines.
The experimental procedure was approved by the local Ethical Committee of the Center for Studies and Investigations in Behavior, by the University of Guadalajara committee for animal experiments, and met governmental guidelines.
8
Operant Conditioning Behavioral Assay
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Operant chambers (N = 7, model ENV-008, Med-Associates, NY) were housed within individual ventilated sound-attenuation cubicles (model ENV-018MD) equipped with white lights (~ 60 lx) and an exhaust fan. The front panels of the operant chambers were outfitted with a retractable lever and a food trough. Food troughs were modified by use of a photobeam to allow for detection of food bin inspections (‘nose-pokes’). Pellets were provided via a 20 mg pellet dispenser positioned outside the chamber and attached to the food trough. Operant chambers were modified to allow for insertion of water bottles and plastic tops were replaced with wire mesh to allow for recording of locomotor activity using overhead infrared motion sensors. Operant responses (lever presses or nose-pokes) and reinforcements were summed and stored in 1-min intervals. Lever operation, reinforcement delivery, and data collection were controlled by a Pentium PC running Med-PC for Windows software (version 4.24; Med-Associates). Activity counts measured by overhead motion sensors were summed and stored in 1-min intervals using the ClockLab data acquisition system (Actimetrics, IL, US).
9
Operant Conditioning Chamber Setup
10
Operant Conditioning Chambers for Behavior Study
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