Eagle 2k ccd camera

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Eagle 2k CCD camera is a high-performance scientific imaging device designed for use in a variety of laboratory applications. It features a 2048 x 2048 pixel CCD sensor with a pixel size of 7.4 μm, providing a large field of view and high spatial resolution. The camera is capable of capturing images with a maximum frame rate of 15 frames per second and offers a 16-bit dynamic range for accurate data acquisition. The camera is compatible with a range of interfaces, including USB 3.0, making it a versatile tool for scientific research and analysis.

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10 protocols using eagle 2k ccd camera

Cryo-TEM Imaging of Liposomes

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The samples were prepared in the same way as described in the Laurdan-stained liposomes preparation without adding Laurdan.
A 4-μL aliquot of sample was deposited on an EM grid coated with a perforated carbon film. After draining the excess liquid with a filter paper, grids were plunge-frozen into liquid ethane cooled by liquid nitrogen using a Leica EMCPC cryo-chamber (Leica Microsystems, Wetzlar, Germany). For cryo-TEM observation, grids were mounted onto a Gatan 626 cryoholder and transferred to a Tecnai F20 microscope (Thermo Fisher Scientific) operated at 200 kV. Images were recorded with an Eagle 2k CCD camera (FEI, Hillsboro, OR, USA).

Bacha K., Chemotti C., Monboisse J.C., Robert A., Furlan A.L., Smeralda W., Damblon C., Estager J., Brassart-Pasco S., Mbakidi J.P., Pršić J., Bouquillon S, & Deleu M. (2022). Encapsulation of Vitamin C by Glycerol-Derived Dendrimers, Their Interaction with Biomimetic Models of Stratum corneum and Their Cytotoxicity. Molecules, 27(22), 8022.

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Purified HTAncLAAO2 Electron Microscopy

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A 3 μl of the purified HTAncLAAO2 (0.5 mg/ml) was applied to a copper grid supporting a continuous thin-carbon film, left for 1 min, and then stained with three drops of on-ice-cooled 2% uranyl acetate. Images of molecules were recorded by an Eagle 2k CCD camera (FEI, Hillsboro, OR) using a Tecnai T20 electron microscope (FEI) operated at an accelerating voltage of 200 kV, at a nominal magnification of ×80,000.

Kawamura Y., Ishida C., Miyata R., Miyata A., Hayashi S., Fujinami D., Ito S, & Nakano S. (2023). Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction. Communications Chemistry, 6, 200.

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Serial Sectioning and Tomography of Drosophila Larval Brains

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Epon-embedded Drosophila larval brains were cut transversally to the ventral nerve cord with Leica Ultracut UCT ultramicrotome. Totally, 200–250 nm thick serial sections were collected on formvar carbon-coated slot grids and 10 nm colloidal gold particles were deposited on both surfaces for fiducials. Samples were imaged on a FEI Tecnai G2 20 operating at 200 kV (Lab6) with a FEI eagle 2k CCD camera at a nominal magnification of 14,500 that resulted in a resolution of 1.5 nm per pixel. FEI single tilt tomography holder was tilted over a range of ±65° according to a Saxton’s scheme (2° starting angle, for a total of 87 images collected) using the FEI Xplore3d acquisition software. Tilted images alignment, tomography reconstruction (WBP) and tomograms joining was done with the IMOD software package^{52 (link)}. ER structures were rendered by manually segmenting the membranes of ER profiles using IMOD software^{53 (link)}.

Espadas J., Pendin D., Bocanegra R., Escalada A., Misticoni G., Trevisan T., Velasco del Olmo A., Montagna A., Bova S., Ibarra B., Kuzmin P.I., Bashkirov P.V., Shnyrova A.V., Frolov V.A, & Daga A. (2019). Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon. Nature Communications, 10, 5327.

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Cryo-EM Analysis of Chemically Induced Protein Oligomers

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CSANs were formed by adding a 1.1-3.0 fold molar excess of the desired chemical dimerizer – either bisMTX or aizde-bisMTX – to a solution of targeted DHFR² fusion protein monomers in PBS. Consistent with our previous studies, CSAN oligomerization occurs within minutes of adding the dimerizer.^{27 (link)} Cryo-EM samples were prepared using a Vitrobot Mark IV (FEI). Briefly, 3 μL of CSANs in PBS was applied to a lacey formvar/carbon grid (Ted Pella, Inc.; Cat: 01883) in a humidified chamber, blotted, and plunged into liquid ethane for vitrification. Grids were imaged on a Tecnai Spirit G2 BioTWIN (FEI) equipped with an Eagle 2k CCD camera (FEI) under a high tension of 120 kV. Images were analyzed in ImageJ and, for the size distribution analysis, only nanoparticles with ≥70% circularity were included. For DLS, 60 μL of CSANs in PBS was loaded into a cuvette and analyzed on a Punk DLS unit (Unchained Labs). Hydrodynamic diameter values represent the mean ± standard deviation of at least three measurements.

Csizmar C.M., Petersburg J.R., Hendricks A., Stern L.A., Hackel B.J, & Wagner C.R. (2018). Engineering Reversible Cell-Cell Interactions with Lipid Anchored Prosthetic Receptors. Bioconjugate chemistry, 29(4), 1291-1301.

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Cryo-EM Sample Preparation Protocol

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A 4 μL aliquot was placed onto an EM grid coated with a perforated carbon film. Excess liquid was removed with filter paper, and grids were plunge-frozen into liquid ethane cooled by liquid nitrogen using a Leica EMCPC cryo-chamber (Leica Microsystems, Milton Keynes, UK). For Cryo-EM observation, grids were mounted onto a Gatan 626 cryoholder (Gatan Inc., Pleasanton, CA, USA) and transferred to a Tecnai F20 microscope (ThermoFisher, Waltham, MA, USA) operated at 200 kV. Images were captured with an Eagle 2k CCD camera (FEI, Hillsboro, OR, USA).

Ferreira W.T., Hong H.A., Hess M., Adams J.R., Wood H., Bakun K., Tan S., Baccigalupi L., Ferrari E., Brisson A., Ricca E., Teresa Rejas M., Meijer W.J., Soloviev M, & Cutting S.M. (2021). Micellar Antibiotics of Bacillus. Pharmaceutics, 13(8), 1296.

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Cryogenic Transmission Electron Microscopy of Polyplexes

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Polyplex
solutions were prepared as described above at an N/P ratio of 5. CryoTEM samples were prepared
using Vitrobot Mark IV (FEI). A total of 3.0 μL of polyplex
solution was applied onto a lacey Formvar/carbon grid (Ted Pella,
Inc.), which was held by a pair of tweezers in humidity controlled
(95%) chamber at 22 °C. After the excess solution was blotted
away using filter paper, the grid was quickly plunged into liquid
ethane. The vitrified samples were then quickly transferred into liquid
nitrogen for storage. For imaging, sample grids were transferred onto
a Gatan 626 cryogenic sample holder in liquid nitrogen and examined
in FEI Tecnai G² Spirit BioTWIN LaB₆ transmission
electron microscope at −178 °C, using an accelerating
voltage of 120 kV. Images were recorded using Eagle 2k CCD camera,
and analyzed with FEI TEM Imaging and Analysis (TIA) software. Phase
contrast was enhanced by imaging at about 10 μm under focus.

Wu Y., Wang M., Sprouse D., Smith A.E, & Reineke T.M. (2014). Glucose-Containing Diblock Polycations Exhibit Molecular Weight, Charge, and Cell-Type Dependence for pDNA Delivery. Biomacromolecules, 15(5), 1716-1726.

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TEM Imaging of Nanoparticle Samples

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The sample morphologies were confirmed by transmission electron microscopy (TEM) with an FEI Tecnai G2 20 X-TWIN equipped with a LaB

_{6}

emission source and an FEI Eagle 2 K CCD camera. The samples for TEM observations were prepared by the protocol commonly used for nanoparticles, described in the literature [46 (link)].

Laskowska M., Kowalczyk P., Karczmarska A., Kramkowski K., Wrzosek K, & Laskowski Ł. (2022). A Novel Biocidal Nanocomposite: Spherical Silica with Silver Ions Anchored at the Surface. International Journal of Molecular Sciences, 24(1), 545.

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Cryo-EM Sample Preparation Protocol

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For the negatively stained specimen, a 3-μl aliquot of sample solution was applied to a glow discharged, continuous thin-carbon film supported by a copper grid, left for 1 min, and then stained with three drops of on-ice-cooled 2% uranyl acetate. Stained samples are examined by a Tecnai T20 electron microscope (FEI, Hillsboro, OR) operated at 200 kV accelerating voltage. Images were recorded using an Eagle 2k CCD camera (FEI). Holey carbon (Quantifoil R1.2/1.3 Au 200) grids were used for frozen-hydrated specimens. Grids were glow discharged for 1 min by an HDT-400 hydrophilic treatment device (JEOL, Tokyo, Japan) before usage. Two-three μL of sample solutions were applied on holey grids for rapid freezing. Rapid freezing was performed using EM-GP (Leica Wetzlar, Germany) or Vitrobot (FEI) freezing robots.

Mayanagi K., Oki K., Miyazaki N., Ishino S., Yamagami T., Morikawa K., Iwasaki K., Kohda D., Shirai T, & Ishino Y. (2020). Two conformations of DNA polymerase D-PCNA-DNA, an archaeal replisome complex, revealed by cryo-electron microscopy. BMC Biology, 18, 152.

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Ultrastructural Imaging of Human Lung Fibroblasts

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Human embryonic lung fibroblast–derived WI-38 cells were imaged by conventional TEM as described earlier (44 (link)). Briefly, cells were grown on glass coverslips, fixed with 2.5% glutaraldehyde and 2% paraformaldehyde (in 0.1 M sodium cacodylate buffer at 25°C), washed in cacodylate buffer (4°C), and stained with 1% osmium tetroxide and 2% uranyl acetate. Samples were dehydrated in cold ethanol and embedded in Epon. Upon removal of the glass coverslips with liquid nitrogen, ultrathin sections (~70 nm) were cut parallel to the coverslip surface and transferred to 200 mesh copper grids. Sections were imaged in a 120 kV FEI Tecnai Spirit T-12 equipped with a 2k Eagle CCD camera (Thermo Fisher Scientific, FEI).

Carter S.D., Hampton C.M., Langlois R., Melero R., Farino Z.J., Calderon M.J., Li W., Wallace C.T., Tran N.H., Grassucci R.A., Siegmund S.E., Pemberton J., Morgenstern T.J., Eisenman L., Aguilar J.I., Greenberg N.L., Levy E.S., Yi E., Mitchell W.G., Rice W.J., Wigge C., Pilli J., George E.W., Aslanoglou D., Courel M., Freyberg R.J., Javitch J.A., Wills Z.P., Area-Gomez E., Shiva S., Bartolini F., Volchuk A., Murray S.A., Aridor M., Fish K.N., Walter P., Balla T., Fass D., Wolf S.G., Watkins S.C., Carazo J.M., Jensen G.J., Frank J, & Freyberg Z. (2020). Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572.

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Visualizing C5b-CD59 Complexes by Electron Microscopy

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Though the concentration after purification was too low to quantify, samples were screened by negative stain electron microscopy for suitability for further structural studies. 2.5 μL of either C5b8-CD59, or C5b9-CD59 complexes were applied to carbon-coated copper grids (Agar Scientific) glow discharged in air. Grids were washed twice with water and then stained with 2% uranyl-acetate and were left to dry. Samples were imaged at a nominal magnification of 50k on a 120 kV Tecnai T12 microscope (Thermo Fisher Scientific) equipped with a 2 K Eagle CCD camera (Thermo Fisher Scientific).

Couves E.C., Gardner S., Voisin T.B., Bickel J.K., Stansfeld P.J., Tate E.W, & Bubeck D. (2023). Structural basis for membrane attack complex inhibition by CD59. Nature Communications, 14, 890.

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