Mouse gait was analyzed using the DigiGait imaging system (version 4.0.0; Mouse Specifics, Framingham, MA, USA). Mice (4- to 13-week–old SK2-KO or WT and 4- to 5-week–old L7-SK2 or WT mice) were placed on a motorized transparent treadmill within a plexiglass compartment. The belt was turned on at an initial speed of 5–10 cm/s. The speed was gradually increased from 5–10 cm/s to a constant test speed of 20–30 cm/s. Digital videos of paw placement were acquired using a camera mounted beneath the treadmill. For each mouse, 2–5 clips of 2.5–5 s each were selected on the basis of continuous movement and absence of gait pauses. Video acquisitions were repeated until a sufficient number of clips were obtained for each speed. The videos were analyzed using DigiGait software (version 14), which automatically processed the video images to calculate gait parameters. Visual inspection of the analysis and manual corrections were systematically performed for each clip. Parameters were exported and further analyzed in Excel (Microsoft), calculating mean values per animal and per speed for fore- and hindpaws separately, pooling together left and right paws.
Digigait imaging system
Manufactured by Mouse Specifics
Sourced in United States
The DigiGait Imaging System is a laboratory equipment that captures and analyzes video recordings of small animal gait. It provides objective quantification of various gait parameters to support research.
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Lab products found in correlation
46 protocols using digigait imaging system
1
Gait Analysis in Transgenic Mice
2
Muscimol Effects on Murine Gait
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Gait dynamics were evaluated in 9–12 week-old mice using the DigiGait imaging system (mouse Specifics Inc, MA, USA) one hour after delivery of 1 μl muscimol (1 mM) or the saline control vehicle into the ADCV. Mice were placed on a motorized transparent treadmill moving at a constant speed of 20 cm/s and required to run. Approximately 5 seconds of digital video of paw placement was collected to provide adequate number of sequential strides for quantification of several gait variables as stride, stance, and swing.
3
Gait Analysis Using DigiGait Imaging System
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Gait data were collected using the DigiGait Imaging System (Mouse Specifics, Inc), an advanced gait analysis system with Ventral Plane Imaging Technology that generates digital paw prints from the animal as it runs on a motorized treadmill (Hampton, Stasko, Kale, Amende, & Costa, 2004 ). This system has been described in detail previously (Hampton et al., 2004 ) and is described in Data S1 .
4
Quantitative Gait Analysis in Mice
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Gait analysis was performed at 16 weeks after DMM surgery using the DigiGait Imaging System (Mouse Specifics, Inc., Boston, MA), as previously described [25 (link), 26 (link)]. Briefly, animals walked on a motor-driven treadmill with a transparent treadmill belt. A high-speed digital video camera was mounted below the transparent treadmill belt. Digital video images of the underside of the animals were captured at up to 150 frames per second. Each animal was allowed to explore the treadmill compartment for ~1 minute with the motor speed set to 0. Approximately 3–6 sec of videography were collected for each mouse to provide more than 7 sequential strides. Only video segments in which the animal walked with a regularity index of 100% were used for image analyses. The walking speed used was 30 cm/s. Gait data are collected and pooled from left and right hind limbs, respectively.
5
Gait Analysis of Mice on Treadmill
6
Gait Analysis of Nerve Injury in Mice
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The DigiGait Imaging System (Mouse Specifics, Inc.) was used to assess gait dynamics before crush injury and 7, 14, 21, 28, 35 days post injury. Control and Dhhcre/+;Ninj2fl/fl mice were placed on a motorized treadmill within a plexiglas compartment. Digital video images were acquired at a rate of 80 frames per second by a camera mounted underneath the treadmill to visualize paw contacts on the treadmill belt. The treadmill was set at a fixed speed of 15 cm/s, which was determined as the baseline for both control and Dhhcre/+;Ninj2fl/fl mice. The paw area was calculated by the DigiGait software.
7
Gait Analysis of Lesioned Mice
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Digital video images of the underside of mice walking at a speed of 10cm/sec were collected and analyzed using the DigiGait Imaging System and software (Mouse Specifics, Boston, MA) as previously described (28 (link)). Mouse paw area over time was used to calculate various parameters of gait. Results of lesioned animals that received tamoxifen were compared to results of lesioned animals that received vehicle.
8
Analyzing Rodent Running Gait Dynamics
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Running gait was assessed using the DigiGait™ Imaging System (Mouse Specifics, Inc., MA). Briefly, each individual mouse was placed within the brightly lit, enclosed plexiglass chamber on a transparent motorized treadmill belt for 5 min to habituate to the chamber. The treadmill was then turned on at a speed of 5 cm/s for 5 s before the belt speed was slowly increased to 22 cm/s over a 10‐s period. A 10 s video of the mouse running at 22 cm/s was recorded at 164 frames/s using a Basler scA640‐74fc digital camera mounted underneath the treadmill. Then, 3–4 s video segments (~10 steps) were analyzed using DigiGait™ Analyzer software version 15 (Mouse Specifics, Inc., MA). Each video was analyzed to quantify forelimb and hindlimb gait parameters, particularly stride length (the average length of a complete step), stride frequency (the average number of steps per second), swing time (the portion of the stride where the paw is moving forward but is not in contact with the treadmill) and stride time (the time taken to complete one step). Data for the left and right limbs were pooled, but forelimbs and hindlimbs were analyzed separately.
9
Gait Analysis of Adult Mice During Locomotion
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We analyzed gait of adult mice during walk/trot locomotion by ventral plane videography as described [40] (link) using DigiGait Imaging System (Mouse Specifics Inc, Watertown, MA). This system enables mice to walk on a motorized transparent treadmill belt, and the software automatically identifies the stance and swing components of stride, and calculates stance width, stride length, step angle, and paw angle. Briefly, we placed the mice on a treadmill belt that moves at a speed of 24.7 cm/s. We collected digital video images of the underside of mice at 150 frames per second.
10
Gait Analysis in Mice Using DigiGait
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