Levofloxacin eye drops

Healthy Belgian rabbits (3–6 months old, 2–2.5 kg) were divided into 4 groups: control group, OGI group, OGI with intravitreal Ranibizumab group, and OGI with intravitreal Conbercept group. Before OGI, general anesthesia was given via intraperitoneal injection of 3.5 mL/kg chloral hydrate (10%), followed by topical anesthesia with oxybuprocaine hydrochloride drops (Benoxil, 4 g/L, Santen, Japan). The OGI model was established by making a 5 mm penetrating wound circumferentially 6.0 mm behind the limbus at the supratemporal quadrant of the right eye, and the wound was sutured 0.5 hours later with aseptic technique. For anti-VEGF treatments, a single dose of 0.25 mg Ranibizumab (25 uL, Genentech, Inc., and Novartis International AG, Basel, Switzerland) or Conbercept (25 uL, Chengdu Kanghong Biotech Co., Ltd., Sichuan, China) was injected into the vitreous of the eyes after OGI at indicated time point (0.5 hours, 3 days, or 7 days), for each time point, n = 5 animals. Levofloxacin eye drops (0.5%, Santen, Japan) were used three times a day for three days before and after the eye injury and intravitreal injections. Two eyes in OGI group were excluded for endophthalmitis as we observed hypopyon or intravitreal empyema about 2 weeks after OGI. No injury was induced in the left eye.

The assays include chloroform (TCM), dimethylformamide (DMF), dimethyl sulfoxide (DMSO) and PHBV, which were purchased from Sigma (USA). Rosiglitazone was obtained from BioVision (USA). Ammonium formate came from Fisher scientific (USA). Acetonitrile was obtained from Merck Millipore (Germany). Filed emission scanning electron microscope SU8010 was obtained from Hitachi (Tokyo, Japan). LC-20A high-performance liquid chromatography (HPLC) were obtained from Shimadzu equipped with an autosampler (Model SIL-20A), a column temperature controller compartment (CTO-10AS) and ultraviolet detector (SPD-M20A). Diamonsil C18 column (5 μ 250 × 4.6 mm) were purchased from Dikma Technologies Inc., China. PH meter came from Denver Instrument, USA. TonoVet were obtain from Icare, Finland. In the vivo experiments, the assays include pentobarbital sodium (30 mg/kg) (Solarbio, Beijing, China), Benoxil (Santen, Japan), Tobramycin Dexamethasone Eye Ointment (Alcon, USA), 0.5% Levofloxacin Eye Drops (Santen, Japan), 4% Paraformaldehyde (Solarbio, Beijing, China), suture line (10-0 nylon; Alcon; USA), 8-0 Vicryl suture (Ethicon; USA), Normal Saline (Second Xiangya Hospital, Changsha, China), Mitomycin C (Sigma, USA), anti-Collagen I antibody (Abcam, UK), anti-α-SMA antibody (Abcam, UK), and anti-connective tissue growth factor (CTGF) antibody (Abcam, UK).

In this study, the method of OHT induction was based mainly on the research of Mittag [22 (link)]. Rats were anesthetic by 1% sodium pentobarbital with the dosage of 0.4ml per 100 g weight. Incision of the conjunctiva exposed two dorsal episcleral veins located near the superior rectus and temporal episcleral vein near the lateral rectus. Then the veins were lift up and cauterized with an ophthalmic cautery carefully. Levofloxacin eye drops (Santen, Osaka, Japan) were used to diminish inflammation. After the conjunctival incision had healed (4-5 days after cauterization), a subconjunctival injection of 100μL of 5-fluorouracil (5-Fu, Haipu Pharmaceutical Co., Shanghai, China) with a concentration of 25mg/mL was also performed by a 29G needle on the EVC eyes. A week after the first injection of 5-Fu, a subconjunctival injection of 100μL of 5-Fu was performed again (only including Gw2, Gw4, and Gw8, because Gw1 had been executed at that time). Sham-operation eyes (SHO eyes) were sham-operated by receiving conjunctival incision and injection of 5-Fu at the same time with EVC eyes but without cauterization. All kinds of Blank-control eyes (BC-I eye, BC-II eye, and BC-III eye) received no treatment.