Cd57 pb

Manufactured by BioLegend

CD57 PB is a fluorescent-labeled antibody that binds to the CD57 antigen, which is expressed on a subset of natural killer (NK) cells and a subset of T cells.

Automatically generated - may contain errors

Lab products found in correlation

Ccr7 bv650, by BD (1 mentions) Cd95 fitc, by BD (1 mentions) Cd4 apc cy7, by BD (1 mentions) Cd45ra ecd, by Beckman Coulter (1 mentions) Cd49d pecy7, by BioLegend (1 mentions) Aria 3, by BD (1 mentions) Allprep dna rna micro kit, by Qiagen (1 mentions) Cd27 pe, by BD (1 mentions) Cd8 apc, by BD (1 mentions) Cd56 ecd, by Beckman Coulter (1 mentions) Nkp46 pe, by BD (1 mentions) Cd14 pecy5, by Beckman Coulter (1 mentions) Aqua live dead kit, by Thermo Fisher Scientific (1 mentions) Cd7 pe cy7, by Beckman Coulter (1 mentions) Cd3 pe cy5, by Beckman Coulter (1 mentions)

2 protocols using cd57 pb

Isolation and Characterization of Naive T Cells

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After thawing, PBMCs were stained with CCR7 BV650 (Clone 3D12; BD Biosciences), CD95 FITC (Clone DX2; BD Biosciences), CD27 PE (Clone M-T271; BD Biosciences), CD8 APC (Clone RPA-T8; BD Biosciences), CD4 APC-Cy7 (Clone RPA-T4; BD Biosciences), CD45RA ECD (Clone IM271111; Beckman Coulter), CD57 PB (Clone HCD57; Biolegend), CD49d PeCY7 (Clone 9F10; Biolegend), and eFluor 506 for viability (Biolegend). Naive CD4⁺ and CD8⁺ T cells, defined as CD45RA⁺, CD27⁺ CCR7⁺, CD95⁻, CD49d⁻, were sorted on an ARIAIII (Becton Dickinson). DNA and RNA from sorted cells were extracted using AllPrep DNA/RNA Micro kit (Qiagen) according to manufacturer instruction.

Nicoli F., Clave E., Wanke K., von Braun A., Bondet V., Alanio C., Douay C., Baque M., Lependu C., Marconi P., Stiasny K., Heinz F.X., Muetsch M., Duffy D., Boddaert J., Sauce D., Toubert A., Karrer U, & Appay V. (2022). Primary immune responses are negatively impacted by persistent herpesvirus infections in older people: results from an observational study on healthy subjects and a vaccination trial on subjects aged more than 70 years old. EBioMedicine, 76, 103852.

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Comprehensive Immune Cell Profiling

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Killer-cell immunoglobulin-like receptor repertoire staining, functional assays, and data analysis were performed as described in detail elsewhere (3 (link)). In brief, freshly thawed PBMCs of patient and controls were stained with the following monoclonal antibodies: CD3-PE.Cy5, CD14-PE.Cy5, CD19-PE.Cy5, CD56-ECD, ILT2-PE, CD161-PE, CD7-PE-Cy7, and NKG2A-APC.AF750, all from Beckman Coulter; NKG2C-PE or biotin from R&D Systems; PLZF-PE, NKp30-APC, and NKp46-PE from Becton Dickinson; FcϵRγ1-FITC from Millipore; and CD57-PB from Biolegend. Dead cells were excluded by using the aqua live/dead kit (Invitrogen).

Béziat V., Sleiman M., Goodridge J.P., Kaarbø M., Liu L.L., Rollag H., Ljunggren H.G., Zimmer J, & Malmberg K.J. (2015). Polyclonal Expansion of NKG2C+ NK Cells in TAP-Deficient Patients. Frontiers in Immunology, 6, 507.

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