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Anti brdu apc

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The Anti-BrdU-APC is a fluorescently labeled antibody that binds to Bromodeoxyuridine (BrdU), a synthetic nucleoside used to label cells undergoing DNA replication. This antibody is conjugated with the fluorescent dye Allophycocyanin (APC), which can be detected using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Brdu labelling reagent, by Thermo Fisher Scientific (2 mentions) Bu20a, by Thermo Fisher Scientific (2 mentions) Facsdiva software, by BD (1 mentions) Anti cd3 bv605, by BioLegend (1 mentions) Anti cd138 percpcy5, by BioLegend (1 mentions) Pacific blue rat igg 2a, by BioLegend (1 mentions) Anti ly 6g pacific blue, by BioLegend (1 mentions) Percp cy5.5 rat igg2b, by BioLegend (1 mentions) Apc rat igg2a, by BioLegend (1 mentions) Apc rat igg2b, by BioLegend (1 mentions) Brdu flow kit, by BD (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) H1299, by American Type Culture Collection (1 mentions) Nocodazole, by Merck Group (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) 5 brdu, by Merck Group (1 mentions) Hu331, by Abcam (1 mentions) L mimosine, by Merck Group (1 mentions) Ym155, by Selleck Chemicals (1 mentions) Bi6727, by Selleck Chemicals (1 mentions)

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Anti-BrdU (20 citations) BrdU-APC (2 citations) Anti-BrdU APC antibody (2 citations)

6 protocols using anti brdu apc

1

Cell Proliferation Analysis via BrdU Incorporation

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Cells were plated at 5 × 105 per well on a 6-well plate in triplicate. BrdU labelling reagent (Life Technologies) was diluted 1:100 in growth media. Cells were labelled for 1 h at 37°C prior to fixation with 1% formaldehyde and stained using anti-BrdU-APC diluted 1:100 (BU20A, Cat# 17-5071, eBiosciences; San Diego, CA) before assessment by flow cytometry. Data was analysed using FlowJo software.
Fong M.Y., Zhou W., Liu L., Alontaga A.Y., Chandra M., Ashby J., Chow A., O’Connor S.T., Li S., Chin A.R., Somlo G., Palomares M., Li Z., Tremblay J.R., Tsuyada A., Sun G., Reid M.A., Wu X., Swiderski P., Ren X., Shi Y., Kong M., Zhong W., Chen Y, & Wang S.E. (2015). Breast cancer-secreted miR-122 reprograms glucose metabolism in pre-metastatic niche to promote metastasis. Nature cell biology, 17(2), 183-194.
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2

Cell Proliferation Analysis via BrdU Incorporation

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Cells were plated at 5 × 105 per well on a 6-well plate in triplicate. BrdU labelling reagent (Life Technologies) was diluted 1:100 in growth media. Cells were labelled for 1 h at 37°C prior to fixation with 1% formaldehyde and stained using anti-BrdU-APC diluted 1:100 (BU20A, Cat# 17-5071, eBiosciences; San Diego, CA) before assessment by flow cytometry. Data was analysed using FlowJo software.
Fong M.Y., Zhou W., Liu L., Alontaga A.Y., Chandra M., Ashby J., Chow A., O’Connor S.T., Li S., Chin A.R., Somlo G., Palomares M., Li Z., Tremblay J.R., Tsuyada A., Sun G., Reid M.A., Wu X., Swiderski P., Ren X., Shi Y., Kong M., Zhong W., Chen Y, & Wang S.E. (2015). Breast cancer-secreted miR-122 reprograms glucose metabolism in pre-metastatic niche to promote metastasis. Nature cell biology, 17(2), 183-194.
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3

Cell Cycle Analysis by Flow Cytometry

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Cell cycle was synchronized with either BI6727 or mimosine or nocodazole. Analyses were performed using standard flow cytometry procedures following either propidium iodide staining alone[37 (link)], or together with BrdU incorporation. These cells were then stained with anti-BrdU-APC (eBioscience, San Diego, CA, USA), as described previously [38 ]. Cells were analyzed using a LSRII flow cytometer. All flow cytometry data were analyzed using FlowJo software (Tree Star, Ashland, OR).
Hong M., Ren M.Q., Silva J., Paul A., Wilson W.D., Schroeder C., Weinberger P., Janik J, & Hao Z. (2017). YM155 Inhibits Topoisomerase Function. Anti-cancer drugs, 28(2), 142-152.
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4

Multiparametric Flow Cytometry of Immune Cell Populations

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FcR blocking was performed with FcR blocking reagent, mouse (Miltenyi Biotec). Antibodies used were anti-Ly-6B.2-Alexa Fluor 700 (Bio-Rad), anti-CD11b-APC, anti-Ly-6G Pacific Blue, anti-CD3-BV605, or anti-CD138-PerCP/Cy5.5 (BioLegend). Levels of apoptosis were assessed by TUNEL staining using the in situ bromodeoxyuridine (BrdU) DNA fragmentation kit (Abcam) followed by staining with anti-BrdU-APC (eBioscience) and flow cytometric analysis. Isotype controls were used accordingly: Alexa Fluor 700 Rat IgG2a, κ (clone RTK2758), APC Rat IgG2a, κ (clone RTK2758), APC Rat IgG2b, κ (clone RTK4530), BV605 Rat IgG2b, κ (clone 17A2), APC/Cy7 Rat IgG2c, κ (clone RTK4174), Pacific Blue Rat IgG2a, κ (clone RTK2758), PerCP/Cy5.5 Rat IgG2b, κ (clone RTK4530) (BioLegend). AbCTM anti-Rat/Hamster Bead kit (Molecular Probes, Thermo Fisher Scientific) was used for single-color compensation. Flow cytometry data were collected on an LSRII flow cytometer with BD FACSDiva software (BD Biosciences).
Lilly C.L., Villa N.Y., Lemos de Matos A., Ali H.M., Dhillon J.K., Hofland T., Rahman M.M., Chan W., Bogen B., Cogle C, & McFadden G. (2016). Ex Vivo Oncolytic Virotherapy with Myxoma Virus Arms Multiple Allogeneic Bone Marrow Transplant Leukocytes to Enhance Graft versus Tumor. Molecular Therapy Oncolytics, 4, 31-40.
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5

BrdU Incorporation Assay for Cell Proliferation

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Cells were incubated with 10 mM BrdU (552598, BD Biosciences, Franklin Lakes, NJ) for one hour at 37oC. The BD BrdU flow kit (552598, BD Biosciences) was used to fix and permeabilize cells prior to DNAse treatment and staining with anti-BrdU-APC (1 in 20, 17-5071-42, eBioscience, San Diego, CA) and Dapi (1 in 1000).
Hettmer S., Schinzel A.C., Tchessalova D., Schneider M., Parker C.L., Bronson R.T., Richards N.G., Hahn W.C, & Wagers A.J. (2015). Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma. eLife, 4, e09436.
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6

Investigating Cell Cycle Regulation

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H1299 was purchased from American Type Culture Collection (ATCC) and cultured in RPMI 1640 (Invitrogen) with 5% FBS (Hyclone), at 37°C in 10% CO2. YM155 (S1130) and BI6727 (S2235) were purchased from Selleck Chemicals. L-mimosine, nocodazole and 5-BrdU were products of Sigma-Aldrich. All antibodies for Western Blot analysis of cell cycle proteins were from Cell Signaling Technology including p21, p27, Cyclin D1, Cdt1, Cyclin A1, Cyclin E1, PCNA and Cyclin B1. Actin B antibody was purchased from Sigma. Anti-BrdU-APC was from eBioscience. HU331 was purchased from Abcam (ab120922).
Hong M., Ren M.Q., Silva J., Paul A., Wilson W.D., Schroeder C., Weinberger P., Janik J, & Hao Z. (2017). YM155 Inhibits Topoisomerase Function. Anti-cancer drugs, 28(2), 142-152.
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