Sox2 y 17

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Sox2 (Y-17) is a protein-specific antibody produced by Santa Cruz Biotechnology. It is designed for the detection of Sox2 by various analytical methods.

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Lab products found in correlation

Gata4 g 4, by Santa Cruz Biotechnology (1 mentions) Alexa flour fluorescent secondary antibodies, by Thermo Fisher Scientific (1 mentions) Oct4 c 10, by Santa Cruz Biotechnology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Nestin, by R&D Systems (1 mentions) Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Tra 1 60, by Abcam (1 mentions) Nanog, by Abcam (1 mentions) Alexa fluor 488 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Sox2 (Y‐17) (sc‐17320; (3 citations) Anti-Sox2 (Y-17; (3 citations)

5 protocols using sox2 y 17

Immunofluorescence Staining of Stem Cell Markers

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Cells were fixed in 4% paraformaldehyde for 20 min at room
temperature and then incubated in blocking buffer (PBS containing
5%BSA and 0.2% Triton X-100) at 37 °C for 1 hr.
Cells were then incubated with primary antibodies diluted in blocking buffer
at 4 °C overnight followed by incubation with secondary antibodies
diluted in blocking buffer at 37 °C for 1 hr. Nuclei were stained
with Hoechst33342 (Invitrogen, 1:10000). Primary antibodies used include the
following: Oct4 (C-10, Santa Cruz, 1:200), Sox2 (Y-17, Santa Cruz, 1:200),
GATA4 (G-4, Santa Cruz, 1:200), Nanog (AF2729, R&D Systems, 1:200),
TUJ1 (Covance, Princeton, NJ, 1:500), Myosin (MF-20, DSHB, 1:50). Alexa
Flour fluorescent secondary antibodies (Invitrogen) were used at a 1:2000
dilution.

Chen X., Wang R., Liu X., Wu Y., Zhou T., Yang Y., Perez A., Chen Y.C., Hu L., Chadarevian J.P., Assadieskandar A., Zhang C, & Ying Q.L. (2017). A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate. Developmental cell, 43(5), 563-576.e4.

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Immunohistochemistry of Neural Markers

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The following primary antibodies were used for section and whole mount immunohistochemistry, at the dilutions indicated: rabbit anti-Fbx2 (1:300, Kato et al., 2005 (link), gift of A. Kato and D. Bredt), goat anti-Sox2 (1:300, Santa Cruz Biotechnology, Sox2 Y-17, cat. no. SC-17320); goat anti-Sox10 (1:100, Santa Cruz Biotechnology, Sox10 N-20, SC-17342); mouse anti-Tuj1 (1:2000, Millipore, Ms X beta III tubulin, MAB5564); goat anti-Jag1 (1:300 Santa Cruz Biotechnology, Jag1 C-20, SC-6011); rabbit anti-COL9A2 (1:100, Atlas Antibodies, HPA056316), and rabbit anti-Pax2 (1:100, Covance).

Hartman B.H., Durruthy-Durruthy R., Laske R.D., Losorelli S, & Heller S. (2015). Identification and characterization of mouse otic sensory lineage genes. Frontiers in Cellular Neuroscience, 9, 79.

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Antibody Panel for Protein Analysis

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The following antibodies were used: ERBB2 (Neu C-18, rabbit polyclonal, Santa Cruz Biotechnology #SC284); phosphor-ERBB2 (P-Neu Try1248, rabbit polyclonal, Santa Cruz #SC12352); phosphor-PI3K (P-PI3-Kinase P85α, rabbit polyclonal, Santa Cruz #SC12929); β-ACTIN (BA3R, mouse monoclonal, ThermoFisher Scientific #MA5–15739); SOX2 (Y-17, goat polyclonal, Santa Cruz #SC17320); MYO7A (H-60, rabbit polyclonal, Santa Cruz #SC25834); JAG1 (C-20, goat polyclonal, Santa Cruz #SC6011); GFP (chicken polyclonal, Abcam, ab13970); RFP (rabbit polyclonal, Rockland #600-401-379); OCM (goat polyclonal, N-19, Santa Cruz #SC7446); PVALB (mouse monoclonal, EMD Millipore #MAB1572). Secondary antibodies were purchased from Jackson Immunoresearch. We used fluorescently-conjugated donkey secondaries and horseradish peroxidase (HRP) conjugated goat secondaries.

Zhang J., Wang Q., Abdul-Aziz D., Mattiacio J., Edge A.S, & White P.M. (2018). ERBB2 signaling drives supporting cell proliferation in vitro and apparent supernumerary hair cell formation in vivo in the neonatal mouse cochlea. The European journal of neuroscience, 48(10), 3299-3316.

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Immunofluorescence Staining of Stem Cells

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Cells were fixed, stained and imaged as previously described [27 (link)]. Primary antibodies used were: OCT4 (1;200; Santa Cruz Biotechnology, Dallas, TX, USA; sc-5279), NANOG (1:100; Abcam, Cambridge, UK; ab21624), SOX2(Y-17) (1:200; Santa Cruz Biotechnology, Dallas, TX, USA; sc-17320), TRA-1-60 (1:300; Abcam, Cambridge, UK; ab16288), NESTIN (1:500; R&D Systems, MAB1259), MUSHASHI (1:500; Abcam, Cambridge, UK; ab21628), FOXA2 (HNF3β) (RY-7; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA; sc-101060), β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300; Millipore, Burlington, MA, USA; AB152). Secondary antibodies used were: Alexa Fluor 488 Goat anti-Mouse IgG (H+L; 1:200; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; A11029), Alexa Fluor 568 Goat anti-Rabbit IgG (H+L; 1:200; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; A11036) and Alexa Fluo 647 Donkey anti-Goat IgG (H+L; 1:200; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; A21447). Image analysis was carried out using Zen Blue confocal software (Carl Zeiss AG, Oberkochen, Germany). All images were processed using Adobe Photoshop CS6 (Adobe Inc. San Jose, CA, USA).

Barbuti P., Antony P., Santos B., Massart F., Cruciani G., Dording C., Arias J., Schwamborn J, & Krüger R. (2020). Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson’s Disease Point Mutation A30P. Cells, 9(9), 2065.

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Immunocytochemical Characterization of Cell Types

Cited 1 time

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β-galactosidase staining and immunochemistry was done as in Neves et al. (2012 (link)). Primary antibodies were rabbit polyclonal neuronal class III β-tubulin (Covance PRB435P100; 1:500), rabbit polyclonal GFP (Torrey Pines 401, 1:400), mouse monoclonal GFP (Invitrogen A11120, 1:400), mouse monoclonal Myo7a (Hybridoma bank, 1:100), goat polyclonal Sox2 (Y-17, Santa Cruz, 1:400) and rabbit polyclonal DsRed (Takara, 632496, 1:400). Secondary antibodies were Alexa Fluor 488-, 555-, 546-, and 594-conjugated anti-mouse, anti-goat and anti-rabbit (Molecular Probes Invitrogen, 1:500).

Gálvez H., Tena J.J., Giraldez F, & Abelló G. (2017). The Repression of Atoh1 by Neurogenin1 during Inner Ear Development. Frontiers in Molecular Neuroscience, 10, 321.

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