Agilent nmr spectrometer

Manufactured by Agilent Technologies

Sourced in United States

The Agilent NMR spectrometer is a laboratory instrument used for nuclear magnetic resonance (NMR) spectroscopy. It measures the absorption and emission of radio frequency radiation by the nuclei of atoms in a sample subjected to a strong magnetic field. The Agilent NMR spectrometer is designed to provide high-resolution data about the molecular structure and chemical environment of the analyzed sample.

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Lab products found in correlation

Profiler, by Chenomx (1 mentions) Tsp d4, by Merck Group (1 mentions) Nmr suite 7.1 professional, by Chenomx (1 mentions) 600 mhz library database, by Chenomx (1 mentions) Advance 600 mhz spectrometer, by Bruker (1 mentions) Chelex, by Merck Group (1 mentions) Spectramax 190, by Molecular Devices (1 mentions) Spectramax m5, by Molecular Devices (1 mentions) N n dimethyl p phenylenediamine, by Merck Group (1 mentions) Synapt g2, by Waters Corporation (1 mentions)

Close spelling variants of this product

NMR spectrometer (77 citations) Agilent 400-MR NMR spectrometer (14 citations) Agilent 600 MHz NMR spectrometer (14 citations) Spectrometers (10 citations) Agilent 500 MHz NMR spectrometer (8 citations) Agilent DD2 600 MHz NMR spectrometer (4 citations) Agilent DD2 500 MHz NMR spectrometer (4 citations) Agilent DD2 500 M NMR spectrometer (3 citations) Agilent NMR system 800 MHz NMR spectrometer (2 citations) Agilent 400 MHz NMR spectrometer (2 citations)

10 protocols using agilent nmr spectrometer

Characterizing Polymer-Chelator Interactions

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1H NMR spectra are recorded at 25 °C with Varian Agilent NMR spectrometer operating at 600 MHz to observe chemical interactions between polymer and chelating agent (DTPA). The NMR samples consisted of water solution of HA-DTPA at different molar ratios (HA/DTPA ranging from 0 to 0.5), with 10% v/v D2O.
Diffusion-ordered NMR Spectroscopy (DOSY) is also performed and the z-gradient strengths (Gz) is varied in 20 steps from 500 to 32500 G/cm (maximum strength). The gradient pulse duration (δ) and the diffusion delay (Δ) are kept constant, 2 ms for δ and ranging from 7 to 1000 ms for Δ. After Fourier transformation and baseline correction, DOSY spectra are processed and analysed using Varian software VNMRJ (Varian by Agilent Technologies, Italy) in order to obtain the values of water self-diffusion coefficient.

De Sarno F., Ponsiglione A.M., Russo M., Grimaldi A.M., Forte E., Netti P.A, & Torino E. (2019). Water-Mediated Nanostructures for Enhanced MRI: Impact of Water Dynamics on Relaxometric Properties of Gd-DTPA. Theranostics, 9(6), 1809-1824.

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NMR Spectrum Acquisition Protocol

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All NMR spectra were acquired using a 600 MHz Agilent NMR spectrometer (Agilent Technologies, CA, USA). A Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with pre-saturation with water was used to suppress the macro-molecule and water peak. The ¹H-NMR spectra were measured using 9.8 µs 90° pulses, an acquisition time of 3.0 s, a total echo time of 64 ms, a relaxation delay of 3.0, 128 scans, and 13 min of total acquisition time.

Kim H.J., Seong E.Y., Lee W., Kim S., Ahn H.S., Yeom J., Kim K., Kwon C.H, & Song S.H. (2021). Comparative analysis of therapeutic effects between medium cut-off and high flux dialyzers using metabolomics and proteomics: exploratory, prospective study in hemodialysis. Scientific Reports, 11, 17335.

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Serum NMR Metabolite Profiling

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Serum samples were measured using a 600.170 MHz Agilent NMR spectrometer (Agilent technologies, Santa Clara, CA, USA). One-dimensional (1D) ¹H-NMR was performed using PRESAT pulse sequence for the suppression of the water signal. Water presaturation was applied during a 2 s relaxation delay, the acquisition time was 1.703 s, and a total of 128 transients were collected for each sample. Two-dimensional (2D) ¹H-¹H correlation spectroscopy (COSY) and ¹H-¹³C heteronuclear single quantum coherence spectroscopy (HSQC) were also performed to clarify the identification of overlapping metabolites. All NMR data were manually phased and the baselines corrected. Metabolites were assigned and quantified using Chenomx NMR Suite 8.4 Professional (Chenomx Inc., Edmonton, AB, Canada) with the metabolite library database and 2D data. For the quantification of metabolites, the peak of TSP at 0.00 ppm was referred. Each metabolite was manually fitting in Chenomx Profiler, and each concentration was calculated from the concentration of TSP.

Yoon D., Choi B.R., Lee Y.S., Han K.S., Kim D, & Lee D.Y. (2020). Serum Metabonomic Research of the Anti-Hypertensive Effects of Ogaja on Spontaneously Hypertensive Rats. Metabolites, 10(10), 404.

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NMR-Based Metabolite Profiling Protocol

Cited 1 time

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The analysis of metabolites was performed using the protocol as described in our previous study [20 (link)] with suitable modification. Approximately 20 mg of each sample was weighted and extracted with acetonitrile/water (1:1, v/v) mixture on ice for 10 min. The samples were centrifuged at 3,000×g for 10 min at 4°C, and the supernatant was collected and freeze-dried. The lyophilized samples were dissolved in 700 μL of deuterated water containing 2 mM 3-trimethylsilyl-2,2,3,3-tetradeuteropropionicacid-d4 (TSP-d4; Sigma-Aldrich, St. Louis, MO, USA) as an internal reference, and then was transferred into a 5 mm NMR tube for analysis. ¹H-NMR spectra were acquired on a 600 MHz Agilent NMR spectrometer (Agilent Technologies, Palo Alto, CA, USA) equipped with 600 MHz 4-mm gHX NanoProbe (Agilent Technologies, Santa Clara, CA, USA) at a ¹H frequency of 599.93 MHz. The ¹H-NMR conditions set were the same as those described in our previous study [20 (link)]. The acquired spectra were phased and then the baseline was corrected and referenced to the TSP-d4 peak using a Vnmrj (version 4.2, Agilent Technologies, USA). Metabolites in the ¹H-NMR spectra were tentatively identified using Chenomx 600 MHz library database and Chenomx NMR Suite 7.1 professional (Chenomx Inc., Edmonton, Canada). The identified metabolites were expressed as percentage of the normalized area.

Hoa V.B., Song D.H., Seol K.H., Kang S.M., Kim H.W., Jang S.S, & Cho S.H. (2022). Half-castration is a newly effective method for increasing yield and tenderness of male cattle meat. Animal Bioscience, 35(8), 1258-1269.

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Salivary Metabolite Profiling by NMR Spectroscopy

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The saliva samples were used for ¹H-nuclear magnetic resonance (NMR)
spectroscopy analysis; 150 μL of saliva sample was mixed with 5 μL
of deuterium oxide (D₂O) containing 20 mM of sodium-3-trimethylsilyl
propionate-2,2,3,3-d₄ (TSP; Sigma Aldrich, St. Louis, MO, USA)
and placed in 4-mm NMR nanotubes. NMR spectra for saliva samples were obtained
by high-resolution magic angle spinning (HR-MAS) NMR using an Agilent NMR
spectrometer (Agilent Technologies, Palo Alto, CA, USA) with a 4 mm gHX
NanoProbe. The spinning rate was 2,050 Hz. A Carr-Purcell-Meiboom-Gill pulse
sequence was used to remove signals generated by water and macromolecules in the
saliva samples. The¹H-NMR spectra were measured with an acquisition
time of 1.704 s, 1 s of relaxation delay, and 10 min and 20 s of total
acquisition time. Chenomx NMR suite 7.1 (Chenoms, Edmonton, AB, Canada) software
was used for the assignment of spectra and quantification of metabolites. The
TSP-d₄ peak (0.0 ppm) was used for calibrating the chemical
shifts as a reference.

Kim B., Kim H.R., Kim K.H., Ji S.Y., Kim M., Lee Y., Lee S.D, & Jeong J.Y. (2021). Effects of acute heat stress on salivary metabolites in growing pigs: an analysis using nuclear magnetic resonance-based metabolomics profiling. Journal of Animal Science and Technology, 63(2), 319-331.

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Amyloid-beta Peptide Characterization

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All reagents were purchased from commercial suppliers and used as received unless otherwise noted. Aβ₄₀ and Aβ₄₂ (the sequence of Aβ₄₂: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA) were purchased from Anaspec Inc. (Fremont, CA, USA). Trace metals were removed from buffers and solutions used in Aβ experiments by treating with Chelex overnight (Sigma–Aldrich, St. Louis, MO, USA). Optical spectra were recorded on an Agilent 8453 UV-visible (UV/Vis) spectrophotometer. Absorbance values for biological assays, including cell viability and antioxidant assays, were measured on a Molecular Devices SpectraMax 190 microplate reader (Sunnyvale, CA, USA). ¹H and ¹³C 1D spectra were recorded using a 400 MHz Agilent NMR spectrometer. 2D NMR spectra were acquired on a Bruker Advance 600 MHz spectrometer equipped with a cryoprobe. More detailed experiments, including the preparation and characterization of the compounds, are described in the Supporting Information.

Beck M.W., Derrick J.S., Suh J.M., Kim M., Korshavn K.J., Kerr R.A., Cho W.J., Larsen S.D., Ruotolo B.T., Ramamoorthy A, & Lim M.H. (2017). Minor Structural Variations of Small Molecules Tune Regulatory Activities toward Pathological Factors in Alzheimer’s disease. ChemMedChem, 12(22), 1828-1838.

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Amyloid-beta Aggregation Inhibition

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All reagents were purchased from commercial suppliers and used as received unless otherwise stated. N,N-Dimethyl-p-phenylenediamine was purchased from Sigma-Aldrich (St. Louis, MO, USA). Aβ₄₀ and Aβ₄₂ were purchased from AnaSpec (Fremont, CA, USA) (Aβ₄₂ = DAEFRHDSGYEVHHQKL-VFFAEDVGSNKGAIIGLMVGGVVIA). An Agilent 8453 UV–visible (UV–vis) spectrophotometer (Santa Clara, CA, USA) was used to measure optical spectra. Anaerobic reactions were performed in a N₂-filled glovebox (Korea Kiyon, Bucheon-si, Gyeonggi-do, Korea). TEM images were taken using a Philips CM-100 transmission electron microscope (Microscopy and Image Analysis Laboratory, University of Michigan, Ann Arbor, MI, USA) or a JEOL JEM-2100 transmission electron microscope (UNIST Central Research Facilities, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea). Absorbance values for cell viability assay were measured on a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). All IM–MS experiments were carried out on a Synapt G2 (Waters, Milford, MA, USA). NMR studies of DMPD with and without Zn(II) were carried out on a 400 MHz Agilent NMR spectrometer. NMR studies of Aβ with DMPD were conducted on a 900 MHz Bruker spectrometer equipped with a TCI triple-resonance inverse detection cryoprobe (Michigan State University, Lansing, MI, USA).

Derrick J.S., Kerr R.A., Nam Y., Oh S.B., Lee H.J., Earnest K.G., Suh N., Peck K.L., Ozbil M., Korshavn K.J., Ramamoorthy A., Prabhakar R., Merino E.J., Shearer J., Lee J.Y., Ruotolo B.T, & Lim M.H. (2015). A Redox-Active, Compact Molecule for Cross-Linking Amyloidogenic Peptides into Nontoxic, Off-Pathway Aggregates: In Vitro and In Vivo Efficacy and Molecular Mechanisms. Journal of the American Chemical Society, 137(46), 14785-14797.

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Characterization and Preparation of PVA-Azosulphonate Compounds

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PVA was characterised prior to use by means of gel-permeation chromatography (GPC) and ¹H-NMR spectroscopy. GPC was performed at the Department of Physical and Theoretical Chemistry at the University of Graz; NMR spectra were recorded with a 400 MHz Agilent NMR spectrometer (Santa Clara, United States). A polydispersity index of 1.32 and a Mw of 130,000 g/mol were determined, and the specified degree of saponification (>99 mol %) was confirmed.
The aqueous PVA solution with a solid content of 5 wt % was obtained by addition of PVA to deionised water under stirring. After 10 min, the slurry was heated to a temperature of 85 °C for at least 60 min to obtain a homogeneous solution.
The respective azosulphonate-doped PVAs (PAII and PAIII) were prepared as follow: The appropriate amount of the azosulphonate salt (Table 1) was dissolved in the 5 wt % aqueous PVA solution at a pH value of 3 adjusted with hydrochloric acid. For a better distribution of the azosulphonates, the samples were treated by sonication and stirred at 60 °C. The solutions were stored at room temperature in brown glass vials to prevent light-induced decomposition of the PVA-AZO compounds.

Nothdurft P., Schauberger J.G., Riess G, & Kern W. (2019). Preparation of a Water-Based Photoreactive Azosulphonate-Doped Poly(Vinyl Alcohol) and the Investigation of Its UV Response. Polymers, 11(1), 169.

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High-Field NMR Characterization Protocol

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Higher-field non-laser-enhanced NMR experiments were performed on a 900 MHz (21.2 Tesla) Agilent NMR spectrometer (Direct Drive2 900 console) equipped with a 5 mm ¹H{¹³C¹⁵N} cryogenic probe. All NMR samples contained 5 % D₂O and 10mM potassium phosphate (pH 7.0). No GO-CAT enzymes nor D-glucose were added to the samples. All measurements were performed at 25 °C.

Okuno Y, & Cavagnero S. (2016). Fluorescein: a Photo-CIDNP Sensitizer Enabling Hyper-Sensitive NMR Data Collection in Liquids at Low Micromolar Concentration. The journal of physical chemistry. B, 120(4), 715-723.

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In Vitro and In Vivo 19F-MRS for 5-FC Monitoring

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For in vitro ¹⁹F-MRS experiments, MSC and MSC/CD cells (1 x 10⁵) were grown in 6 well plates. Cells were then incubated in growth medium containing 100 μM of 5-FC (Kolon Life Science, Gyeonggi, Korea). After 24 hours, conditioned media were collected and analyzed using 9T (375.567 MHz) Agilent NMR spectrometer (Agilent Technologies, Santa Clara, CA, USA). Chemical phantom samples were constructed by diluting 5-FC (Kolon Life Science, Gyeonggi, Korea) and 5-FU (Sigma-Aldrich, MI, USA) to 200 uM each in 5ml of DMEM with 10% FBS. Spectra were acquired with a single pulse sequence whole region excitation with 11 min scan time (average 512). The ratio of peak intensities was measured using VnmrJ 3.2 version software program (Agilent Technologies, Santa Clara, CA, USA).
For in vivo ¹⁹F-MRS experiments, MSC/CD cells (1x10⁶) were subcutaneously injected into the right flank of mice. Serial ¹⁹F-NMR spectra were acquired every 8.5 min during 0 to 1.5 h (total repetition time, 500 ms; the number of averages, 1024; spectral width, 25 kHz; acquisition size, 2048 points) after administration of 5-FC (500 mg/kg, intraperitoneally). The chemical shift of the 5-FC resonance was set to 0 ppm and the 5-FU signal was observed at 1.2 ppm. The signal positions were verified with phantom samples of 5-FC and 5-FU in the culture medium which were described above.

Chung T., Na J., Kim Y.I., Chang D.Y., Kim Y.I., Kim H., Moon H.E., Kang K.W., Lee D.S., Chung J.K., Kim S.S., Suh-Kim H., Paek S.H, & Youn H. (2016). Dihydropyrimidine Dehydrogenase Is a Prognostic Marker for Mesenchymal Stem Cell-Mediated Cytosine Deaminase Gene and 5-Fluorocytosine Prodrug Therapy for the Treatment of Recurrent Gliomas. Theranostics, 6(10), 1477-1490.

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