Flurbiprofen flb

Dobendan Direkt lozenges (Boots Healthcare, Hamburg, Germany) were provided by Klosterfrau Healthcare Group, Köln, Germany. Water HPLC-grade, acetonitrile HPLC-grade, and methanol HPLC-grade were from Roth, Karlsruhe, Germany. Human CYP2C9*1 Supersomes were from BD Biosciences, Woburn, USA. Flurbiprofen (FLB) was purchased from Sigma-Aldrich, Steinheim, Germany, flurbiprofen-d₃ (FLB-d₃) was from TRC, Toronto, Canada. 4-Hydroxyflurbiprofen was a gift from the Dept. of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, USA. Primers were purchased from Invitrogen GmbH, Darmstadt, Germany. LongAmp Taq polymerase and restriction endonucleases AvaII, NsiI, and KpnI together with the respective ready-to-use buffers were purchased from New England Biolabs, Frankfurt a. Main, Germany.

Cladophora cellulose (CLAD) was provided by FMC Biopolymers (currently DuPont, Philadelphia, PA, USA). Microcrystalline cellulose (MCC) (Avicel PH101), ibuprofen (IBU), Ibuprofen-D3 (IBU-D3); Naproxen (NAP), Flurbiprofen (FLB), and Ketoprofen (KET) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Biorelevant media of simulated gastric fluid (SGF), fasted simulated intestinal fluid (FaSIF), and fed state simulated intestinal fluid (FeSIF) were prepared using powder purchased from Biorelevant (London, UK) according to the manufacturer’s instructions. The chemical structures of studied profens are presented in Table 1. Table 1 summarizes the physical chemical properties of the arylpropionic acid derivaties under study.