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Mlt0202

Manufactured by ADInstruments
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Sourced in Australia, Spain

The MLT0202 is a high-performance force transducer designed for a variety of research applications. It features a compact size, versatile mounting options, and a durable stainless steel construction. The transducer is capable of measuring force in both compression and tension, making it a suitable choice for a wide range of experimental setups.

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Lab products found in correlation

Powerlab 8 30, by ADInstruments (3 mentions) Fe224, by ADInstruments (3 mentions) Pl3516 p, by ADInstruments (3 mentions) Powerlab, by ADInstruments (2 mentions) Labchart, by ADInstruments (1 mentions) Mla1213, by ADInstruments (1 mentions) Mla0320, by ADInstruments (1 mentions) Stable temp hotplate, by Cole-Parmer (1 mentions) Ml 870, by ADInstruments (1 mentions)

10 protocols using mlt0202

1

Proximal Colon Muscle Contractility Assay

Cited 1 time
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Strips of longitudinal (6 mm in length) muscle from proximal colon were mounted in a 37 °C organ bath with a force transducer (MLT0202; AD Instruments, Spain) connected to a PowerLab (AD Instruments, Australia) recording device. Briefly, Ca2+-free HEPES-Tyrode (H-T) buffer (140.6 mmol/L NaCl, 2.7 mmol/L KCl, 1.0 mmol/L MgCl2, 10 mmol/L HEPES, and 5.6 mmol/L glucose, pH 7.4) was used to wash out the lumen contents of proximal colon segments. Then, segments were transferred to the organ bath and equilibrated in H-T buffer (137.0 mmol/L NaCl, 2.7 mmol/L KCl, 1.0 mmol/L MgCl2, 1.8 mmol/L CaCl2, 10 mmol/L HEPES, and 5.6 mmol/L glucose, pH 7.4) for 15 min. The resting tension was set to 0.5 g prior to force measurement. The isotonic contractions of longitudinal muscle was recorded for 1 h, with H-T buffer being changed every 15 min. The mean amplitude of the basal tension and the frequency of phasic contractions were measured for 30 min after experiments. The detailed force assays were performed according to our previously described methods [26 (link), 27 (link)].
Ge X., Ding C., Zhao W., Xu L., Tian H., Gong J., Zhu M., Li J, & Li N. (2017). Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility. Journal of Translational Medicine, 15, 13.
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2

Tracheal Ring Isometric Tension Assay

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The rats were sacrificed after anesthetizing by 1.6 g/kg intraperitoneal (i.p.) administration of urethane and their chests opened. Tracheal rings of rats containing three cartilages were prepared from the middle section of trachea as previously described [24 (link)] and mounted in a 10 ml organ bath containing Krebs-Henseleit solution supplied with 95% O2 and 5% CO2 and tissue responses were measured using an isometric transducer (MLT0202, AD Instruments, Australia) connected to a power lab system (Power Lab 8/30, ML870, AD Instruments, Australia) exactly as previously described [24 (link)–26 (link)].
Kazemi Rad H., Memarzia A., Amin F, & Boskabady M.H. (2021). Relaxant Effect of Urginea maritima on Tracheal Smooth Muscle Mediated by the Effect on Beta-2 Adrenergic, Muscarinic Receptors and Calcium and Potassium Channels. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 6637990.
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3

In Vitro Contractility Measurements of Mouse Intestinal Segments

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Force measurements were performed as described.17 (link) The mice were killed by cervical dislocation. Segments (6 mm long) of the jejunum and ileum were mounted in a 37°C organ bath and tied with surgical silk to the hooks of a force transducer (MLT0202; AD Instruments, Sydney, Australia) and lengthadjusting micrometer. After equilibrating in H-T buffer (137.0 mmol/L NaCl, 2.7 mmol/L KCl, 1.0 mmol/L MgCl2, 1.8 mmol/L CaCl2, 10 mmol/L HEPES, and 5.6 mmol/L glucose, pH 7.4) for 15 minutes, the contractile effect of KCl (87 mM), acetylcholine (Ach) (1 mM) and electrical field stimulation (EFS) (20 Hz, 30 V, pulse train, 20 seconds) was investigated.
Qin X., Liu S., Lu Q., Zhang M., Jiang X., Hu S., Li J., Zhang C., Gao J., Zhu M.S., Feil R., Li H., Chen M., Weinstein L.S., Zhang Y, & Zhang W. (2016). Heterotrimeric G Stimulatory Protein α Subunit Is Required for Intestinal Smooth Muscle Contraction in Mice. Gastroenterology, 152(5), 1114-1125.e5.
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4

Electrical Stimulation and Contractile Force of Tissue Constructs

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At the time of tissue construct formation, spontaneous and electrically paced twitch forces (10 V, 0.1 s) were measured within a thermostated water bath (37°C) using a high-sensitivity isometric force transducer (MLT0202, ADInstruments, Colorado Springs, CO, USA) connected to a quad bridge amplifier (FE224, ADInstruments). ECG signals were recorded using a Bio Amp (FE136, ADInstruments) as previously described.39 (link) Data were acquired through a 16-channel PowerLab system (PL3516/P, ADInstruments). The ECG of the tissue construct was detected by inserting a needle cathode (MLA1213, ADInstruments) into the center of the construct and a needle anode in one of the four construct corners. The media immersing the tissue was used as a ground. The contractile twitch force was measured by attaching the force transducer arm to one free-corner of the square construct, while the other three ends of the square construct were held fixed by pins. Pre-tension was adjusted using a micro-manipulator (Radnoti LLC, Monrovia, CA, USA), set at 1000–2000 μN, force during spontaneous contraction was recorded. LabChart (ADInstruments) was used for data analysis. Electrical pacing was performed by attaching stimulating electrodes (MLA0320, ADInstruments) to the edge of the tissue and pacing at frequencies of 0.25 and 1–5 Hz (in 1-unit increments).
Tao Z.W., Mohamed M., Jacot J.G, & Birla R.K. (2018). Bioengineering Cardiac Tissue Constructs with Adult Rat Cardiomyocytes. ASAIO journal (American Society for Artificial Internal Organs : 1992), 64(5), e105-e114.
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5

Measuring Colon Muscle Contractility

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Strips of longitudinal (6mm in length) muscle from the proximal colon were mounted in a 37 °C organ bath with a force transducer (MLT0202; AD Instruments, Spain) connected to a PowerLab (AD Instruments, Australia) recording device. Briefly, Ca2+-free HEPES-Tyrode (H-T) buffer (140.6 mmol/L NaCl, 2.7 mmol/L KCl, 1.0 mmol/L MgCl2, 10 mmol/L HEPES, and 5.6 mmol/L glucose, pH 7.4) was used to wash out the lumen contents of proximal colon segments. Next, the segments were transferred to the organ bath and equilibrated in H-T buffer (137.0 mmol/L NaCl, 2.7 mmol/L KCl, 1.0 mmol/L MgCl2, 1.8 mmol/L CaCl2, 10 mmol/L HEPES, and 5.6 mmol/L glucose, pH7.4) for 15 minutes. The resting tension was set to approximately 0.5 g prior to force measurement. The isotonic contractions of longitudinal muscle were recorded for 1 hour, with H-T buffer being changed every 15 minutes. The mean amplitude of the basal tension and frequency of phasic contractions were measured for 30 minutes after experiments. The detailed force assays were performed according to our previously described methods54 (link), 55 (link).
Ge X., Zhao W., Ding C., Tian H., Xu L., Wang H., Ni L., Jiang J., Gong J., Zhu W., Zhu M, & Li N. (2017). Potential role of fecal microbiota from patients with slow transit constipation in the regulation of gastrointestinal motility. Scientific Reports, 7, 441.
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6

Measuring Contractile Twitch Force of AHM

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Example 10

Contractile Twitch Force of AHM

From day 4, twitch force was measured using a high sensitivity isometric force transducer (MLT0202, ADinstruments, Colorado Springs, Colo.), connected to a quad bridge amplifier (FE224, ADinstrument, Colorado Springs, Colo.). Data acquisition was performed through a 16 channel PowerLab system (PL3516/P, ADInstruments, Colorado Springs, Colo.). The force transducer arm was attached to one free-corner of the patch, while the other ends were held fixed; spontaneous measurements were recorded for 30-60 seconds. Pretension was adjusted using a micro-manipulator (Radnoti LLC, Monrovia, Calif.) and measurements of spontaneous contraction were recorded. A Stable-Temp hotplate (Cole-Parmer, Vernon Hills, Ill.) was used to maintain media temperatures at 37° C. throughout the course of measurement. LabChare's (ADInstruments, Colorado Springs, Colo.) peak analysis module was used to calculate the maximum twitch force and baseline force (pretension).

US10792145B2. Two stage cellularization strategy for the fabrication of bioartificial hearts (2020-10-06). None [US]. Inventors: Ravi K. Birla [US].
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7

Tracheal Smooth Muscle Contractility Assay

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Rats were sacrificed and the chest was opened to obtain the trachea. The trachea was dissected and excess connective tissue and fat were removed. Then, the trachea was cut into rings of 3–4 mm width each having about four cartilages for the formation of tracheal ring. Each tracheal ring was hung between two Nichrome hooks inserted into the lumen, and placed in a 10 mL organ bath containing Krebs-Henseliet solution (KHS; composition (mM): NaCl 120, KCl 4.72, KH2PO4 1.2, MgSO4·7H2O 0.5, CaCl2·2H2O 2.5, NaHCO3 25 and dextrose 11). This solution was maintained at 37 ± 0.5 °C and constantly bubbled with 5% CO2-95% O2. Tissue was suspended under isotonic tension of 1 g and allowed to equilibrate for at least 1 h while it was being washed with KHS solution every 15 min. In all experiments, contraction responses were measured using an isotonic transducer (MLT0202, AD Instruments, Australia) which was connected to a power lab system (PowerLab 8/30, ML870, AD Instruments, Australia).
Emami B., Shakeri F., Ghorani V, & Boskabady M.H. (2017). Relaxant effect of Curcuma longa on rat tracheal smooth muscle and its possible mechanisms. Pharmaceutical Biology, 55(1), 2248-2258.
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8

Vascular Pressure and Contraction Monitoring

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A Millar Catheter (3.0 French; AD Instruments, Inc) was used to record pressure in the thoracic aorta. It was inserted through a small cut into the vessel from the distal ligated end and tied in place after it was adjusted to rest approximately 1cm from the proximal ligature. While pressure changes in the aorta was one method of recording spontaneous contractions, in some experiments, they were recorded using an isometric force transducer (MLT0202, AD Instruments) connected by a 4.0 silk thread attached to the aorta. Blood pressure was also monitored by cannulation of the carotid artery, which was done to keep us informed about the physiological condition of our experimental preparation during the course of the experiment, and to confirm that a subset of rats were successfully bled. To monitor the ECG, differential recordings were made using two needle electrodes placed subcutaneously, one in the right forelimb and the other placed in the left hindlimb (low pass filter setting at 100 Hz).
Sahibzada N., Mangel A.W., Tatge J.E., Dretchen K.L., Franz M.R., Virmani R, & Gillis R.A. (2015). Rhythmic Aortic Contractions Induced by Electrical Stimulation In Vivo in the Rat. PLoS ONE, 10(7), e0130255.
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9

Isolated Rat Trachea Contractility Assay

Cited 2 times
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For obtaining the trachea, the rats were sacrificed by a blow on the neck and the chest was opened and excess of connective tissue and fat were dissected out (Holroyde 1986 ). A piece of the trachea with 5–6 cartilage rings was isolated and mounted between two stainless steel hooks in 10 mL organ bath containing Krebs-Henseleit solution, and maintained at 37 ± 0.5 °C with isometric tension of 1 g as previously described (Saadat, Yasavoli, et al. 2019 (link)). The isometric transducer (MLT0202, AD Instruments, Australia) linked to a power lab system (Power Lab 8/30, ML870, AD Instruments, Australia) was used to measure the contraction and relaxation responses in all experiments (Emami et al. 2017 (link)).
Emami B., Shakeri F., Gholamnezhad Z., Saadat S., Boskabady M., Azmounfar V., Sadatfaraji H, & Boskabady M.H. (2020). Calcium and potassium channels are involved in curcumin relaxant effect on tracheal smooth muscles. Pharmaceutical Biology, 58(1), 257-264.
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10

Contractile Behavior of Engineered Cardiac Tissue

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From days 2-3, at macroscopic contraction observation, twitch force of the constructs was measured using a high sensitivity isometric force transducer (MLT0202, ADInstruments), connected to a quad bridge amplifier (FE224, ADInstruments). Data acquisition was through a 16 channel PowerLab system (PL3516/P, ADInstruments). The force transducer arm was attached to one free corner of the AVEM, while the other three ends were held fixed by minutien pins; spontaneous measurements were recorded for 20-60 seconds. The contractility of AVEM patches was collated over the 14 days culture period, at 4 to 5 day intervals, to map the contractile frequency behaviour of the construct. In order to obtain the Frank-Starling relationship, pretension was adjusted using a micro-manipulator (Radnoti LLC, Monrovia, CA) and measurements of spontaneous contraction were recorded. LabChart was used for data analysis with the peak analysis module, to calculate maximum twitch force and baseline force (pretension).
Patel N.M., Tao Z.W., Mohamed M.A., Hogan M.K., Gutierrez L, & Birla R.K. (2015). Engineering 3D Bio-artificial Heart Muscle: The Acellular Ventricular Extracellular Matrix Model. ASAIO journal (American Society for Artificial Internal Organs : 1992), 61(1), 61-70.
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