Thapsigargin tp

Manufactured by Merck Group

Thapsigargin (TP) is a natural product derived from the plant Thapsia garganica. It is a potent inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) enzyme, which plays a crucial role in the regulation of intracellular calcium homeostasis. TP is commonly used as a research tool in cell biology and biochemistry studies to investigate calcium signaling pathways and the function of the endoplasmic reticulum.

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Lab products found in correlation

β tubulin 3 antibody, by Merck Group (1 mentions) Sodium phenylbutyrate 4 pba, by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) Il 1β, by Miltenyi Biotec (1 mentions) Il 1 receptor antagonist il 1ra, by R&D Systems (1 mentions) Tnf α, by BioLegend (1 mentions) Birb0796, by Selleck Chemicals (1 mentions) Syk inhibitor r406, by Selleck Chemicals (1 mentions)

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Thapsigargin (790 citations)

2 protocols using thapsigargin tp

Antibodies and Reagents for Cell Signaling

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Mouse monoclonal antibodies against GAPDH and actin, rabbit polyclonal antibodies against PTEN, PERK, phosphorylated elF2 and CHOP were from Cell Signaling Technologies. β-tubulin III antibody, thapsigargin (TP) and sodium phenylbutyrate (4-PBA) were from Sigma-Aldrich. Anti-Phosphorylated PERK antibody was obtained from Santa Cruz Biotechnology. Aβ₁₋₄₂ was purchased from China Peptide. The mimic or inhibitors of miR-200 family and siPTEN/control siRNAs were synthesized by Life Technologies with sequences shown in Table 1.

Wu Q., Ye X., Xiong Y., Zhu H., Miao J., Zhang W, & Wan J. (2016). The Protective Role of microRNA-200c in Alzheimer's Disease Pathologies Is Induced by Beta Amyloid-Triggered Endoplasmic Reticulum Stress. Frontiers in Molecular Neuroscience, 9, 140.

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Dectin-1 Pathway Modulation in mDC

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10μg/ml mouse IgG_2B dectin-1 blocking antibody (clone-259931 R&D Systems), 10ug/ml mouse IgG_2B isotype control (clone-20116 R&D Systems), 5μM Syk inhibitor, R406 (Selleckchem), 10μM NFκB inhibitor, isohelenin (Calbiochem), 1μM p38 MAPK inhibitor, BIRB0796 (Selleckchem), 5μg/ml anti-TNFα, Infliximab (JannsenBiotech), 1μg/ml IL-1 receptor antagonist (IL-1RA) (R&D Systems), 50μM caspase-1 inhibitor (Z-YVAD-FMK) (Calbiochem), 50μM caspase-8 inhibitor (Z-IE(OMe)TD(OMe)-FMK) (Calbiochem), 10ng/ml TNFα (Biolegend), 10ng/ml IL-1β (Miltenyi), 1μg/ml tunicamycin (TM) (Sigma-Aldrich), 0.25μM thapsigargin (TP) (Sigma-Aldrich), 30μM IRE1α inhibitor, 4μ8C (Calbiochem) and 1μM GSK-PERK inhibitor (Toronto Research). Where inhibitors, blocking antibodies and modifiers were used, mDC were pre-treated one hour prior to cell stimulation. Repeated experiments were performed on independent donors unless otherwise stated.

Elder M.J., Webster S.J., Williams D.L., Gaston J.S, & Goodall J.C. (2015). TSLP production by dendritic cells is modulated by IL‐1β and components of the endoplasmic reticulum stress response. European Journal of Immunology, 46(2), 455-463.

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