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Xpert c difficile epi pcr assay

Manufactured by Cepheid

The Xpert® C. difficile/Epi PCR assay is a real-time PCR diagnostic test designed to detect the presence of Clostridioides difficile (formerly known as Clostridium difficile) and its ribotype 027 (epidemic) strain in stool samples. The assay provides rapid and accurate results to aid in the diagnosis of Clostridioides difficile infection.

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2 protocols using xpert c difficile epi pcr assay

1

Post-Transplant Diarrhea Diagnostic Workup

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Post-transplant diarrhea was defined as a complaint of diarrhea and by the World Health Organization classification of 3 or more unusually loose/liquid bowel movements per day 9 . Workup for infectious etiologies for post-transplant diarrhea by the clinician was not performed in all reported cases. Potential standard of care testing included the Xpert® C. difficile/Epi PCR assay (Cepheid, Inc., Sunnyvale, CA), bacterial fecal culture, examination for ova and parasites, norovirus RT-PCR (Viracor Eurofins Clinical Diagnostics, Lee’s Summit, MO), and/or BioFire® FilmArray® GI Panel testing (which was initiated in-house at our institution in May 2016).
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2

Xpert C. difficile PCR Assay Evaluation

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The Xpert C. difficile/EPI PCR assay (Cepheid, Sunnyvale, CA) was performed on 27 stool samples further selected from the Illumigene/LAMP-BART method comparison for low copy number, high inhibitor load, blood content and less common ribotype. The GeneXpert C. difficile cartridge was used as described by the manufacturer with the exception that formed stools were tested to assess the test’s ability to detect C. difficile in formed stool.
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