Firstly, 11,12-Epoxyeicosatrienoic acid (11,12-EET) 11,12-dihydroxyeicosatrienoic acid (11,12-DHET), 14,15-Epoxyeicosatrienoic acid (14,15-EET), 14,15-dihydroxyeicosatrienoic acid (14,15-DHET), 9,10-epoxyoctadecamonoenic acid (9,10-EpOME), 9,10-dihydroxyoctadecenoic acid (9,10-DiHOME), 12,13-epoxyoctadecamonoenic acid (12,13-EpOME), 12,13-dihydroxyoctadecenoic acid (12,13-DiHOME), prostaglandin (PG) E2, PGD2, PGI2, PGH2, 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT), malondialdehyde (MDA), 19,20-epoxydocosapentaenoic acid (19,20-EDP), and 19,20-dihydroxydocosapentaenoic acid (19,20-DHDP) were from Cayman Chemical (Ann Arbor, MI, USA). Acetonitrile (ultra LC–MS grade), ethanol (HPLC gradient grade), formic acid (p.a grade), and isopropanol (LC–MS grade) were from Roth (Karlsruhe, Germany), and ethyl acetate (LC grade) was from Merck (Darmstadt, Germany). All other compounds including flufenamic acid were from Sigma–Aldrich (Steinheim, Germany).
+ Open protocol