Cryostat system em uc7 fc7

TEM was further used to determine the subcellular changes associated with Slit2 in the post-IR myocardium. Left ventricular tissues were cut into small blocks (about 1 mm³), fixed with 2.5% glutaraldehyde, fixed with 1% OsO₄, dehydrated in ethanol, and embedded in Araldite. The tissue blocks were cut into slices at a thickness of 60 nm using a Leica cryostat system (EM UC7/FC7, Germany) and collected on copper grids. The ultrathin sections were double stained with 3% uranyl acetate and lead citrate. The subcellular structure was observed with a Tecnai G2 Spirit transmission electron microscope (FEI Company, United States). During evaluation of the subcellular differences between the groups, different technicians performed the slice preparation and observations, and the technicians did not know the sample IDs.

TEM was conducted to examine subcellular changes associated with n-3 PUFA expression. Left ventricular tissues were cut into small blocks (~1 mm³), fixed with 2.5% glutaraldehyde and 1% OsO₄, dehydrated in ethanol, and embedded in Araldite. The tissue blocks were cut into slices with a thickness of 60 nm using a Leica cryostat system (EM UC7/FC7) and collected on copper grids. The ultrathin sections were double-stained with 3% uranyl acetate and lead citrate. The subcellular structure was observed using a Tecnai G2 Spirit transmission electron microscope (FEI Company, Hillsboro, OR, USA).