Rabbit anti yap1

Manufactured by Cell Signaling Technology

Sourced in Canada

Rabbit anti-YAP1 is a primary antibody that specifically binds to the YAP1 protein. YAP1 is a transcriptional coactivator that plays a key role in the Hippo signaling pathway, which regulates cell proliferation and apoptosis.

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Lab products found in correlation

Alexa fluor 594 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Mouse anti e cadherin, by BD (1 mentions) Rabbit anti vimentin, by Abcam (1 mentions) Mouse anti ki67, by BD (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Rabbit anti mmp 9, by Abcam (1 mentions) Mouse anti vimentin, by BD (1 mentions) Eukitt mounting medium, by Avantor (1 mentions) Lats1 2, by Fortis Life Sciences (1 mentions) Mouse anti e cadherin, by Cell Signaling Technology (1 mentions) Rabbit anti cyclin d1, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Mouse anti β catenin, by BD (1 mentions) Rabbit anti phospho β catenin, by Cell Signaling Technology (1 mentions) Rabbit anti brd4, by Cell Signaling Technology (1 mentions) Rabbit anti cyr61, by Cell Signaling Technology (1 mentions) Rabbit anti glyceraldehyde 3 phosphate dehydrogenase, by Cell Signaling Technology (1 mentions) Alexa 488 phalloidin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-YAP1 (47 citations) Rabbit anti-YAP1 (4912; (2 citations)

7 protocols using rabbit anti yap1

Immunohistochemical Analysis of EMT Markers

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Primary antibodies used were as follows: Rabbit anti-YAP1 (#14074, Cell signaling Technology, Whitby, ON, Canada); Mouse anti-vimentin (#550513), Mouse anti-E-Cadherin (#610181) and Mouse anti-Ki67 (#556003) were from BD BIOSCIENCES (Franklin Lakes, NJ, USA); Rabbit anti-ZEB1 (#ABD53), Rabbit anti-total Fibronectin (#F3648), Mouse anti-laminin-V (#MAB19562), and Mouse pan-anti-Cytokeratin AE1/AE3 (#MAB3412) were from MilliporeSigma (Billerica, MA, USA); Rabbit anti-vimentin (#ab45939), Rabbit anti-MMP9 (#ab76003) were from Abcam (San Francisco, CA, USA). Secondary antibodies used were as follows: Goat anti-Mouse Alexa Fluor 488 (#A11001), Goat anti-Rabbit Alexa-Fluor 488 (#A11029), Goat anti-Rabbit Alexa-Fluor 594 (#A11037), and Goat anti-Mouse Alexa-Fluor 594 (#A11032) were from Thermo Fisher Scientific. All other chemicals were from MilliporeSigma unless specified.

Millet M., Bollmann E., Ringuette Goulet C., Bernard G., Chabaud S., Huot M.É., Pouliot F., Bolduc S, & Bordeleau F. (2022). Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition. Cancers, 14(15), 3810.

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Immunohistochemical Analysis of LATS2 and YAP1

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Tissue sections (3-μm thick) were prepared from formalin-fixed, paraffin-embedded human tissues and underwent standard immunohistochemistry protocols for LATS2 (rabbit anti-LATS1/2, 1:100, 2 h; Bethyl), YAP1 (rabbit anti-YAP1, 1:100, 1 h; Cell Signaling), or H pylori (rabbit anti–H pylori 1:100, 2 h, cat. B0471; DAKO) antibodies, and then with horseradish-peroxidase–labeled anti-rabbit EnVision System (30 min; DAKO).¹⁷ Immunolabeling was shown after a 10-minute incubation in liquid diaminobenzidine-chromogen substrate (cat. K3468; DAKO). Slides were counterstained with hematoxylin, dehydrated, and mounted with Eukitt-mounting medium (VWR). Relative quantification of the percentage of gastric epithelial cells expressing nuclear LATS2 and nuclear YAP1 was determined by a double-blind scoring using a scale from 1 to 4 (0, 0%; 1, <5%; 2, 5%–25%; 3, 25%–50%; and 4, >50%).

Molina-Castro S.E., Tiffon C., Giraud J., Boeuf H., Sifre E., Giese A., Belleannée G., Lehours P., Bessède E., Mégraud F., Dubus P., Staedel C, & Varon C. (2019). The Hippo Kinase LATS2 Controls Helicobacter pylori-Induced Epithelial-Mesenchymal Transition and Intestinal Metaplasia in Gastric Mucosa. Cellular and Molecular Gastroenterology and Hepatology, 9(2), 257-276.

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Western Blotting of Tumor Tissue Lysates

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Primary antibodies used included rabbit anti–phospho-β-catenin (#4176), rabbit anti-cyclin D1 (#55506), rabbit anti-YAP1 (#4912), rabbit anti-CYR61(#39382), rabbit anti-KEAP1 (#4678), mouse anti–E-cadherin (#5296), rabbit anti-BRD4 (#13440), and rabbit anti–glyceraldehyde-3-phosphate dehydrogenase (#5174) from Cell Signaling Technology; rabbit anti-active YAP1 (ab205270) and rabbit anti-NRF2 (ab137550) from Abcam; mouse anti–β-catenin (610154) from BD Transduction Laboratories; rabbit anti–poly(adenosine diphosphate–ribose) polymerase 1/2 (sc-7150) from Santa Cruz Biotechnology; and mouse anti–β-actin (A5441, Sigma-Aldrich). Anti-mouse (#7076) and anti-rabbit (#7074) horseradish peroxidase–linked secondary antibodies were purchased from Cell Signaling Technology. Tumor tissues were dissociated/processed into single cells at 3% O₂ and ambient air, and we lysed/removed the red blood cells. Cell lysates were prepared in radioimmunoassay buffer and analyzed by Western blotting as described previously (55 (link)).

Kumar B., Adebayo A.K., Prasad M., Capitano M.L., Wang R., Bhat-Nakshatri P., Anjanappa M., Simpson E., Chen D., Liu Y., Schilder J.M., Colter A.B., Maguire C., Temm C.J., Sandusky G., Doud E.H., Wijeratne A.B., Mosley A.L., Broxmeyer H.E, & Nakshatri H. (2022). Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity. Science Advances, 8(2), eabh3375.

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Comprehensive Antibody Characterization for Cell-Based Studies

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The following primary antibodies were used: rabbit anti-NOS1AP (Novus Biologicals, NBP2-38758 and NBP2-38151), guinea anti-nephrin (Progen, GP-N2), mouse anti-SMA (Sigma-Aldrich, A2547), mouse anti-CD31 (MA3100, Thermo Fisher Scientific), mouse anti-NWASP (LSBio, LS-C133098-100), mouse anti-DIAPH3 (Proteintech, 14342-1-AP), rabbit anti-c-Myc (Sigma-Aldrich, C3956), mouse anti-myc (Santa Cruz Biotechnology, SC-40), mouse horseradish peroxidase (HRP)–linked anti–β-actin (Abcam, ab20272), rabbit anti-YAP1 (Cell Signaling Technology, 4912), anti-Golgin B1 (GOLGB1) (Sigma-Aldrich, HPA011008), anti-GRP78 Binding Immunoglobulin Protein (BiP) (Abcam, ab21685), anti-Nucleoporin 153 (NUP153) (Sigma-Aldrich, HPA027897), and anti–cleaved CASP3 (Abcam, ab2302). Donkey anti-mouse, anti-guinea, and anti-rabbit Alexa 488– and Alexa 594–conjugated secondary antibodies; 4′,6-diamidino-2-phenylindole (DAPI) staining reagents; and phalloidin–Alexa 488 were obtained from Invitrogen (Thermo Fisher Scientific). HRP-labeled secondary antibodies were purchased from Santa Cruz Biotechnology.

Majmundar A.J., Buerger F., Forbes T.A., Klämbt V., Schneider R., Deutsch K., Kitzler T.M., Howden S.E., Scurr M., Tan K.S., Krzeminski M., Widmeier E., Braun D.A., Lai E., Ullah I., Amar A., Kolb A., Eddy K., Chen C.H., Salmanullah D., Dai R., Nakayama M., Ottlewski I., Kolvenbach C.M., Onuchic-Whitford A.C., Mao Y., Mann N., Nabhan M.M., Rosen S., Forman-Kay J.D., Soliman N.A., Heilos A., Kain R., Aufricht C., Mane S., Lifton R.P., Shril S., Little M.H, & Hildebrandt F. (2021). Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Science Advances, 7(1), eabe1386.

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Protein Lysate Analysis by Western Blot

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Protein lysate was prepared in SDS/Tris (pH7.6) lysis buffer, separated by electrophoresis in 8–10% SDS/PAGE gels, transferred to nitrocellulose membrane, and probed with the following antibodies: rabbit anti-YAP1 (4912; 1:1000), rabbit anti-p-Yap (Ser397) (13619; 1:1000), rabbit anti-GAPDH (2118; 1:1000), mouse anti-AMOT (60156–1-lg 1:500), rabbit anti-V5-tag (13202; 1:1000), rabbit anti-p65 (8242; 1:500), rabbit anti-p-p65 (Ser536) (3033; 1:500), rabbit anti-Caspase-3 (9662; 1:1000), rabbit anti-HSP90 (4875; 1:1000) (Cell Signaling Technology), rabbit USP31(12076–1-AP; 1:1000), rabbit anti-MYOD1 (18943–1-AP; 1:500) (Proteintech), rabbit FOXM1 (sc-502; 1:500) (Santa Cruz Biotechnology), c-Myc (32072; 1:2000), p57 Kip2 (75974; 1:500) (Abcam), mouse anti-Lamin B2 (D18) (University of Iowa Developmental Studies Hybridoma Bank). SuperSep Phos-tag™ gel was purchased from Wako. Rabbit anti-LATS1 (3477; 1:1000), rabbit anti-MST1 (3682; 1:1000), rabbit anti-MST2 (3952; 1:1000) (Cell Signaling Technology) were used to detect phosphorylated proteins.

Ye S., Lawlor M., Rivera-Reyes A., Egolf S., Chor S., Pak K., Ciotti G., Lee A., Marino G., Shah J., Niedzwicki D., Weber K., Park P.M., Alam M.Z., Grazioli A., Haldar M., Xu M., Perry J.A., Qi J, & Eisinger-Mathason T.S. (2018). YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis. Cancer research, 78(10), 2705-2720.

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Immunostaining Pancreatic Cell Markers

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Primary antibodies used were rabbit anti-Ngn3 (1:100; Acris), rat anti-E-cadherin (1:400; Zymed), rabbit anti-C-peptide (1:200; Linco), rabbit anti-Amylase (1:300; Sigma), rabbit anti-Ptf1a (1:3,000; Gift from B. Breant), rat anti-Cytokeratin19 (1:250; DSHB), mouse anti-Glucagon (1:500; Sigma), rabbit anti-Pdx1 (1:5,000; Gift from C. Wright), mouse anti-Pdx1 (1:250; DSHB), rabbit anti-Sphk (1:250; Abcam), mouse anti-Nkx6-1 (1:1,000; DSHB), mouse anti-Insulin (1:1,000; Sigma), rabbit anti-PH3 (1:500; Cell Signaling), rabbit anti-Yap1 (1:200; Cell Signaling), rabbit anti-NICD (1:100; Cell Signaling), rat anti-Hes1 (1:500; MBL-Biozol), and rabbit anti-Sel1l (1:250; Abcam). Secondary antibodies were anti-mouse, anti-rabbit, and anti-rat Alexa-488-, Alexa-568-, and Alexa-633-conjugated goat antibodies (1:500; Molecular Probes), as well as anti-rabbit and anti-rat biotinylated goat antibodies (1:100; Vector). For Nkx6.1 and Pdx1 mouse antibody staining together with Sphk, a Goat anti-Mouse IgG affiniPure Fab Fragment from Jackson ImmunoResearch (115-007-003) was used at 1:50 dilution following the instructions of the manufacturer.

Serafimidis I., Rodriguez-Aznar E., Lesche M., Yoshioka K., Takuwa Y., Dahl A., Pan D, & Gavalas A. (2017). Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling. PLoS Biology, 15(3), e2000949.

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Liver Protein Extraction and Western Blotting

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Total liver and isolated hepatocyte lysates were prepared using RIPA buffer with protease inhibitor cocktail (Sigma-Aldrich Corp. St Louis, MO). For western blotting, primary antibodies used included rabbit anti-pSmad2 (Abcam or Cell Signaling as above), goat anti-E-cadherin (R & D), rabbit anti-albumin (Santa Cruz), rabbit anti- α SMA (Abcam), and rabbit anti-Yap1 (Cell Signaling). HRP-conjugated anti-rabbit or mouse (GE Healthcare, Pittsburgh, PA) and donkey anti-goat (Santa Cruz) were used as secondary antibodies. Signal was developed using ECL Prime (GE Healthcare) with detection on a Chemidoc system (BioRad) or using DAB reagent (DAKO).

Oh S.H., Swiderska-Syn M., Jewell M.L., Premont R.T, & Diehl A.M. (2018). Liver regeneration requires Yap1-TGFβ-dependent epithelial-mesenchymal transition in hepatocytes. Journal of hepatology, 69(2), 359-367.

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