C57bl 6j b6j mice

Subjects were adult (8–10 weeks of age) female (~20 g) and male (~30 g) C57BL/6J (B6J) mice (catalog no. 000664; n = 12 mice/sex/concentration), obtained from The Jackson Laboratory (Sacramento, CA, USA). Mice were housed in same-sex groups of 4 and allowed a minimum of 7 days to acclimate to a climate- and humidity-controlled colony room, under a reverse 12 h light/dark cycle (lights off at 11:00 h). Mice were identified using tail markings, and food and water were available ad libitum. All the cages were lined with sawdust bedding, with nesting materials and an igloo in accordance with vivarium protocols, and the experimental procedures complied with the Guide for the Care and Use of Laboratory Animals (2014), as approved by the Institutional Animal Care and Use Committee of the University of California, Santa Barbara (protocol number 829.4).

Adult (P60–P120) male mice were used in this study. CC003/Unc, CC008/GeniUnc, CC009/Unc, and CC029/Unc (referred herein as CC003, CC008, CC009, and CC029, respectively) mice were acquired from the Systems Genetics Core Facility at the University of North Carolina at Chapel Hill between 2016 and 2020.^{18 (link)} The classic laboratory inbred C57BL/6J (B6J) mice (#000664) were purchased from The Jackson Laboratory and used as non-fatal controls because they are resistant to seizure-induced sudden death in flurothyl challenge. All mice were raised on standard mouse chow and kept on a 12:12 light/dark cycle. All studies were compliant with Institutional Animal Care and Use Committee protocols.