Anti klf8

Manufactured by Merck Group

Sourced in China

Anti-KLF8 is a laboratory reagent used in molecular biology research. It is an antibody that specifically binds to the Krüppel-like factor 8 (KLF8) protein, which is a transcription factor involved in various cellular processes. The primary function of Anti-KLF8 is to facilitate the detection and study of KLF8 expression and localization in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti klf8

Protein Expression Analysis in Primary Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary cell cultures were homogenized in NP40 buffer (Beyotime, China) supplemented with PMSF (1 mM, Sigma-Aldrich). The cell lysates were subjected to western blotting using the anti-Caspase3 (1:1000, 9662 s, Cell Signaling Technology), anti-KLF8 (1:500, 2,101,276, Sigma-Aldrich), anti-Flag (1:1000, 14,793, Cell Signaling Technology) and HRP-conjugated anti-GAPDH (Kangcheng, Shanghai, China) antibodies. The protein bands were analyzed using Image Lab analysis (Bio-Rad).

Wang M.D., Xing H., Li C., Liang L., Wu H., Xu X.F., Sun L.Y., Wu M.C., Shen F, & Yang T. (2020). A novel role of Krüppel-like factor 8 as an apoptosis repressor in hepatocellular carcinoma. Cancer Cell International, 20, 422.

+ Open protocol

+ Expand

Western Blotting of Mammosphere Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting procedures were carried out as previously described (15 ). Briefly, cells were collected in RIPA lysis buffer (150mM NaCl, 1% NP40, 0.5% DOC, 50mM Tris-HCl at pH 8, 0.1% SDS, 10% glycerol, 5mM EDTA, 20mM NaF and 1mM Na₃VO₄, 1 μg/ml each of pepstatin, leupeptin, and aprotinin, 200 μg/ml phenyl-methylsulfonyl-fluoride). Lysates were cleared by centrifugation at 14,000 x g for 20 minutes at 4 °C and analyzed by SDS-PAGE and autoradiography. Lysates from mammospheres were collected 7 days after culture in mammosphere media. Corresponding adherent controls were put on mammosphere media 24 hrs after seeding and collected the next day. Antibodies were purchased from the following companies; Anti-OGT (Santa Cruz, Cell-Signaling), Anti-O-GlcNAc (Santa Cruz, Sigma), Anti-c-Myc (Novus), Anti-Actin (Santa Cruz), Anti-CD44, Anti-Fibronectin, Anti-Vimentin, Anti-NANOG (Cell-Signaling), Anti-K8/18 (Thermo-Fisher), Anti-K14 (Thermo-Fisher), Anti-GAPDH (Genscript), Anti-KLF8 (Sigma). Densitometry was performed using Image J Software (National Institutes of Health, Bethesda, MA).

Akella N.M., Le Minh G., Ciraku L., Mukherjee A., Bacigalupa Z.A., Mukhopadhyay D., Sodi V.L, & Reginato M.J. (2020). O-GlcNAc Transferase Regulates Cancer Stem-like Potential of Breast Cancer Cells. Molecular cancer research : MCR, 18(4), 585-598.

+ Open protocol

+ Expand

Protein Expression Analysis in Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected and washed three-times with ice-cold PBS to remove residue media. Then cell pellets were resuspended in cold RIPA lysis buffer (150mM NaCl, 1% NP40, 0.5% DOC, 50mM Tris-HCl at pH 8, 0.1% SDS, 10% glycerol, 5mM EDTA, 20mM NaF and 1mM Na3VO4), supplemented with protease inhibitors. Cell debris was removed by centrifugation at 15000 rpm for 20 minutes at 4°C using benchtop centrifuge. Protein concentration was determined flowing Bradford assay. 50 µg of protein from each sample was separated by SDS-PAGE and transferred to PVDF membrane. Target proteins were detected using indicated specific antibodies: Anti-OGT (Cell-Signaling, cat #20438), Anti-O-GlcNAc (Sigma, cat #MABS1254), Anti-c-MYC (Novus, cat #NB600-335), Anti-Actin (Santa Cruz, cat #sc-47778), Anti-NANOG (Cell-Signaling, cat #3580), Anti-SOX2 (Cell-Signaling, cat #3579), Anti-OCT4 (Cell-Signaling, cat #2750), Anti-KLF8 (Sigma, cat #AV32859).

Le Minh G., Esquea E.M., Dhameliya T.T., Merzy J., Lee M.H., Ball L.E, & Reginato M.J. (2023). Kruppel-like factor 8 regulates triple negative breast cancer stem cell-like activity. Frontiers in Oncology, 13, 1141834.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!