Antibiotic mixture

Human recombinant TGF-β1, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphnyl-2H-tetrazolium bromide (MTT), avertin, and 4′6-diamidino-2-phenylinodole dihydrochloride (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Bleomycin hydrochloride was purchased from Nippon Kayaku Co., Ltd. (Tokyo, Japan). Antibodies against phospho-Smad2 (pSmad2) (S465/467, #3108, 1:1000) and pSmad3 (S423/425, #9520, 1:1000) from Cell Signaling (Danvers, MA, USA); α-SMA (14-9760-82, 1:1000) from Invitrogen (Carlsbad, CA, USA); and Smad2 (sc-101153, 1:1000), Smad3 (sc-101154), and β-actin (1:1000) from Santa Cruz Biotechnology (Dallas, TX, USA) were purchased. Peroxidase-labeled anti-rabbit (sc-2004, 1:1000) and anti-mouse immunoglobulins (sc-2005, 1:1000) were obtained from Santa Cruz Biotechnology. Alexa Fluor 647-conjugated secondary antibody and Dako Faramount Aqueous Mounting Media were purchased from Abcam (Cambridge, MA, USA) and Dako (Carpinteria, CA, USA), respectively. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic mixture were obtained from WELGENE (Daegu, Republic of Korea). All other chemicals were purchased from Sigma-Aldrich.

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic mixtures were purchased from WELGENE (Gyeongsan-si, Gyeongsangbuk-do, Korea). Kojic acid, phenylthiourea (PTU), mushroom tyrosinase, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), α-MSH, SB203580, and SP600125 were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Antibodies against tyrosinase, MITF, JNK, phospho-JNK (p-JNK), p38, phospho-p38 (p-p38), and anti-β-actin were obtained from the Santa Cruz Biotechnology (Santa Cruz, CA, USA). Peroxidase-labelled anti-rabbit and anti-mouse immunoglobulins were obtained from KOMA Biotechnology (Seoul, Korea). All other chemicals were purchased from Sigma grades.