The bone samples obtained were scanned using a micro-CT analyzer (Inveon™ Multi-Modality; Siemens Medical Solutions, Inc., Malvern, PA, USA). The scanning thickness was 9.1 μm at 80 kV and 500 μA. The three-dimensional (3D) image of each sample was reconstructed using the Inveon™ Image Research Workplace (Siemens, German). Furthermore, the volume/total volume (BV/TV) and trabecular number (Tb.N) values were analyzed.
Inveon image research workplace
Manufactured by Siemens
Sourced in Germany
The Inveon Image Research Workplace is a laboratory equipment designed for imaging and analysis. It provides a controlled environment for researchers to conduct their studies. The core function of this product is to enable researchers to capture and analyze images as part of their research activities.
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Lab products found in correlation
3 protocols using inveon image research workplace
1
Micro-CT Analysis of Bone Samples
2
Preclinical PET/CT Imaging Protocol
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Before PET/CT imaging, mice were fasted for 12 h and anesthetized with 1.5% isoflurane. Anesthetized mice were injected intravenously with 18‐FDG (150 µCi) via the tail vein. PET acquisitions were performed immediately after 18‐FDG injection. The images were acquired for 10 min using an Inveon multimodality PET/CT system and reconstructed using the 3D maximum expectation maximization method (Inveon Image Research Workplace; Siemens Healthcare, Erlangen, Germany). The standardized uptake value (SUV) for the region of interest was measured by dividing the 18‐FDG activity by the injected dose and animal weight using the Inborn Research Workplace Software provided by the manufacturer.
3
Micro-CT Analysis of Femoral Bone in Rats
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A total of 24 male rats (Sprague Dawley) were used in this study. The animal experiment was conducted in accordance with the principles of the Animal Experiment Ethics Committee of Nankai Hospital and the international standards on animal welfare. A hole (2 mm in diameter) was drilled into the distal surface of the femoral condyles. After implantation of the columnar samples for 8 and 12 weeks, the rats were sacrificed, and the femurs were collected and fixed in formalin. The femurs were scanned using micro computed tomography (Micro-CT) (Viva CT40, SCANCO Medical AG), and the three-dimensional (3D) image of each sample was reconstructed using the Inveon Image Research Workplace (Siemens, Germany). The bone volume percentage (BV/TV), trabecular number (Tb·Th), and bone mineral density (BMD) were analyzed. After Micro-CT scanning, the femur specimens were dehydrated with ethanol, embedded in polymethyl methacrylate, and cut into sections by a saw microtome (EXAKT Apparatebau, Germany). The sections were stained with Van Gieson's stain and imaged through a stereoscopic microscope.
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