Raman spectroscopy is a non-destructive vibrational technique using a monochromatic laser beam. Scattered light produced by molecular vibrations reveals bands at specific frequencies in the Raman spectrum and allows analysis of the composition of a sample. The paraffin blocks of elbows were trimmed, and the triceps brachii tendon composition was studied using a Renishaw InVia Qontor microscope. This was completed by using a 785 nm monochromatic laser diode (Renishaw Plc, Wotton-under-Edge, UK) with a laser power of 10 mW and a 20× objective. Three spectra were recorded for each tendon with an integration time of 5 s and 15 accumulations. Enzymatic collagen cross-linking (intensity ratio 1670/1690 cm−1), non-enzymatic collagen cross-linking represented by the pentosidine content (Intensity ratio 1345/920 cm−1), hydroxyproline content (Intensity ratio 872/920 cm−1), glycosaminoglycan content (intensity ratio 1380/920 cm−1) and nanoporosity (Intensity ratio 1296/920 cm−1) were computed. The spectra were processed with MATLAB software (The MathWorks, Inc., Natick, MA, USA).
Invia qontor microscope
Manufactured by Renishaw
The InVia Qontor is a high-performance Raman microscope designed for advanced materials analysis. It features a modular and flexible platform that can be configured to meet specific research or industrial needs. The InVia Qontor provides high-resolution imaging and spectroscopic capabilities for a wide range of applications.
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3 protocols using invia qontor microscope
1
Raman Spectroscopic Analysis of Tendon Composition
2
Raman Spectroscopy of Material Samples
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Samples on stubs were analysed using a Renishaw inVia Qontor microscope with a 532 nm laser. Three points of interest were analysed to account for potential variability within the sample. Raman spectra were imported into the software Renishaw WiRE 5.3 and processed as follows: spikes were removed and the range 1100–1700 cm−1 was selected. Finally, the three spectra were smoothed and merged.
3
In Situ Electrochemical Characterization
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In situ measurements were conducted using a three-electrode electrochemical flow cell with a quartz window from GaossUnion (Tianjin) Photoelectric Technology Company (Fig. S1, ESI †). The counter and reference electrodes were graphite and Ag/AgCl (1 M KCl), respectively. The cell was filled with either Ar-saturated 0.1 M HCl or Ar-saturated 0.5 M Na 2 SO 4 via the inlet port. All electrochemical tests were performed on a Metrohm AutoLab PGSTAT302N potentiostat. The potentials against reference were converted to the reversible hydrogen electrode (RHE) using E (vs. RHE) = E (vs. Ag/AgCl) + 0.235 + 0.0591 × pH. The Raman spectroscopy was collected using a Renishaw inVia Qontor microscope equipped with a 785 nm laser, a 50 × L objective lens, an 1800 lines per mm grating, and a CCD detector. Signal acquisition time for each spectrum is about 5 seconds at 5% laser power.
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