Cd34 apc clone 581

Manufactured by BD

Sourced in Germany

CD34-APC (clone 581) is a fluorescent-labeled antibody that binds to the CD34 antigen, a transmembrane glycoprotein expressed on the surface of hematopoietic stem and progenitor cells. It is used for the identification and enumeration of CD34+ cells in flow cytometry applications.

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Lab products found in correlation

Cd45 apc clone hi30, by BD (2 mentions) Facsaria fusion cell sorter, by BD (1 mentions) Cd73 pe clone ad2, by BD (1 mentions) Cd105 fitc clone 266, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Accutase, by STEMCELL (1 mentions) Cd31 pe clone wm59, by BD (1 mentions) Cd14 apc clone m5e2, by BD (1 mentions) Cd33 apc clone ac104.3e3, by Miltenyi Biotec (1 mentions) Cd29 pe clone mar4, by BD (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Transwell permable supports, by Corning (1 mentions) Mouse igg1 isotype control, by R&D Systems (1 mentions)

Spelling variants of this product

CD34-APC (76 citations) CD34 (clone 581, (7 citations) CD34 (581) (6 citations)

4 protocols using cd34 apc clone 581

Characterization of Mesenchymal Stromal Cells

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Cells were incubated with antibodies diluted as suggested by the suppliers in 200 μl of PBS containing 2% FBS (wash buffer) for 30 min on ice, washed in wash buffer, and analyzed using a FACSAria Fusion cell sorter (BD Biosciences). For analysis of iMSCs, CD73-PE (clone AD2, BD Pharmingen), CD105-FITC (clone 266, BD Pharmingen), CD90-PE-Cy7 (clone 5E10, BD Pharmingen), CD34-APC (clone 581, BD Pharmingen), and CD45-APC (clone HI30, BD Pharmingen) were used. The results were analyzed using BD FlowJo software (v.10).

Elhussieny A., Nogami K., Sakai-Takemura F., Maruyama Y., Takemura N., Soliman W.T., Takeda S, & Miyagoe-Suzuki Y. (2021). Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR). Stem Cell Research & Therapy, 12, 532.

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Immunophenotypic Analysis of Stem Cells

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Flow cytometric analysis of hematopoietic surface markers was analyzed on a FACS Canto II (BD Biosciences, Franklin Lakes, New Jersey, USA) with the following antibodies: CD3-PerCP-Cy5.5 (clone UCHT1), CD19-FITC (clone HIB19), CD31-PE (clone WM59), CD34-APC (clone 581), CD38-PE (clone HIT2), CD43-FITC (clone 1G10), CD14-PE (clone MOP9), CD66b-PE (clone G10F5) (all BD Bioscience), CD45-APC-Cy7 (clone 2D1), CD117-PE-Cy7 (clone 104D2), CD11c-PE-Cy7 (clone 3.9), HLA-DR-FITC (clone LN3), CD235a-Pacific Blue (clone 6A7M) (all eBioscience, San Diego, California, USA), CD33-APC (clone AC104.3E3; Miltenyi Biotec). EBs were dissociated with Accutase (Stemcell Technologies, Vancouver, British Columbia, Canada) for 10–15 min prior to staining with antibodies. Single cells were incubated with 1% human IgG solution (Privigen, CSL Behring, King of Prussia, Pennsylvania, USA) for 30 min at 4 °C to block unspecific binding.
Immunophenotypic analysis of MSCs and iMSCs was performed with CD14-APC (clone M5E2), CD29-PE (clone MAR4), CD31-PE (clone WM59), CD34-APC (clone 581), CD45-APC (clone HI30), CD73-PE (clone AD2), CD90-APC (clone 5E10) (all BD Biosciences), and CD105-FITC (clone MEM-226; ImmunoTools, Friesoythe, Germany). Data was analyzed using the FlowJo software (Tree Star, Ashland, Oregon, USA).

Cypris O., Frobel J., Rai S., Franzen J., Sontag S., Goetzke R., Szymanski de Toledo M.A., Zenke M, & Wagner W. (2019). Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells. Clinical Epigenetics, 11, 19.

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Cytokine-mediated Leukemia Stem Cell Expansion

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Cells from primary leukemic samples were stained with antibodies to CD34-APC (clone 581, BD Biosciences, San Jose, CA), and lineage antibodies, including CD2 (clone TS1/8, Biolegend, San Diego, CA), CD3 (clone S4.1PB), CD14 (clone clone TüK4), and CD19 (clone SJ25-C1)-Pacific Blue (Invitrogen, Carlsbad, CA), as well as Propidium Iodide (PI: Molecular Probes, Eugene, OR). Lineage negative (Lin-) CD34+ cells were sorted on FACSAria II cell sorter (BD, Franklin Lakes, NJ) and 2.5 ×10⁵ cells were co-cultured with an equal number of irradiated MSCs in 24-well flat bottom plates with or without cytokines (150 ng/ml FLT3-ligand, 150 ng/ml Stem cell factor (SCF), 50ng/ml Interleukin-3 (IL-3)). In control wells, Lin-CD34+ cells were cultured without MSC support in the presence or absence of the same cytokines. In all wells, culture media were replaced twice weekly. In transwell assays, sorted Lin-CD34+ cells were placed in the transwell insert (Costar Transwell® Permable Supports: 0.4μm pore size) with or without MSCs plated in the lower compartment.

Ito S., Barrett A.J., Dutra A., Pak E., Miner S., Keyvanfar K., Hensel N.F., Rezvani K., Muranski P., Liu P., Larochelle A, & Melenhorst J.J. (2014). Long Term Maintenance of Myeloid Leukemic Stem Cells Cultured with Unrelated Human Mesenchymal Stromal Cells. Stem cell research, 14(1), 95-104.

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DNAM-1 Expression Profiling

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DNAM-1 mAb clone 102511 and mouse IgG1 isotype control were from R&D Systems and BD Biosciences, respectively. All fluorescent antibody conjugates (CD33-BV421 clone WM53; CD34-APC clone 581; CD117-PE-Cy7 clone 104D2) were from BD Biosciences and used in 1:200 dilution, secondary goat-anti–mouse-PE was from Dako. Recombinant human CD112 or CD155 Fc-domain fusion proteins (rhCD112/rhCD155) were from BioLegend. Recombinant proteins were tested to be free of endotoxins by ENDONEXT EndoZyme II assay (bioMérieux).

Chashchina A., Märklin M., Hinterleitner C., Salih H.R., Heitmann J.S, & Klimovich B. (2021). DNAM-1/CD226 is functionally expressed on acute myeloid leukemia (AML) cells and is associated with favorable prognosis. Scientific Reports, 11, 18012.

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