Hcpepic

Manufactured by ScienCell

Sourced in United States

HCPEpiC is a primary human choroid plexus epithelial cell line. It is intended for use in research and investigation of the human choroid plexus and related applications.

Automatically generated - may contain errors

Lab products found in correlation

Epithelial cell growth supplement, by ScienCell (4 mentions) Biolite cell culture flasks, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by ScienCell (2 mentions) Poly l lysine (pll), by ScienCell (2 mentions) Penicillin streptomycin solution, by ScienCell (2 mentions) Epithelial cell medium, by ScienCell (2 mentions) Erk1 2 9102, by Cell Signaling Technology (1 mentions) Akt 4691, by Cell Signaling Technology (1 mentions) Fak 3285, by Cell Signaling Technology (1 mentions) Gsk3β 9315, by Cell Signaling Technology (1 mentions) Cell extraction buffer, by Thermo Fisher Scientific (1 mentions) Penicillin, by ScienCell (1 mentions) Streptomycin, by ScienCell (1 mentions) Qiazol lysis reagent, by Qiagen (1 mentions) Biolite 6 well plates, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Molecular Research Center (1 mentions) Hcmec d3, by ScienCell (1 mentions) X vivo 15 medium, by Lonza (1 mentions) Human astrocyte, by ScienCell (1 mentions) Human neurons, by ScienCell (1 mentions)

6 protocols using hcpepic

Culturing Human Keratinocyte and Choroid Plexus Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HaCaT cells, a human keratinocyte epithelial cell line (America Type Culture Collection) were cultured under standard conditions as reported^{15 (link)}. The primary human choroid plexus epithelial cells (HCPEpiCs) (ScienCell Research Laboratories) were cultured in EpicM epithelial cell medium added with 1% epithelial cell growth supplement (ScienCell Research Laboratories) and 2% fetal bovine serum (FBS). HCPEpiCs cells were used within three cell-culture passages to exclude de-differentiation to mesenchymal cells.
Chemical reagents were from Sigma, if not specified. Human plasma purified Cp was from Enzo Life Sciences. The antibodies used were: anti-ceruloplasmin (ab8813, Abcam); the antibodies used for the signaling analysis (FAK #3285, p-Tyr³⁹⁷FAK #3283, ERK1/2 #9102, p-Thr^202/Tyr²⁰⁴ERK1/2 #9101, GSK3β #9315, p-Ser⁹GSK3β #9336, AKT #4691, p-Ser⁴⁷³AKT #193H12) were from Cell Signaling Technology, while anti-β-tubulin antibody (T6199) was from Sigma-Aldrich.

Barbariga M., Zanardi A., Curnis F., Conti A., Boselli D., Di Terlizzi S, & Alessio M. (2020). Ceruloplasmin oxidized and deamidated by Parkinson's disease cerebrospinal fluid induces epithelial cells proliferation arrest and apoptosis. Scientific Reports, 10, 15507.

+ Open protocol

+ Expand

Primary Epithelial Cell Culturing

Check if the same lab product or an alternative is used in the 5 most similar protocols

HCPEpiCs were obtained from ScienCell Research Laboratories and cultured in epithelial cell medium according to the distributor's recommendations. HCPEpiCs at passages 3–5 were used for the experiments. HEK293 cells were purchased from Humanitas Clinical and Research Center (Rozzano, Milan, Italy) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum.

Zhao Y., Zeng C., Li X., Yang T., Kuang X, & Du J. (2020). Klotho overexpression improves amyloid‐β clearance and cognition in the APP/PS1 mouse model of Alzheimer's disease. Aging Cell, 19(10), e13239.

+ Open protocol

+ Expand

Primary Choroid Plexus Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human primary choroid plexus epithelial cells (HCPEpiC, ScienCell, Carlsbad, CA, USA) were cultured in epithelial cell medium containing 2% fetal bovine serum, 1% epithelial cell growth supplement, 100 units/ml penicillin and 100 μg/ml streptomycin (all ScienCell, Carlsbad, CA, USA). When cells reached approximately 90% confluence pooled CSF from 4 preterm infants with IVH, metHb and heme (prepared immediately prior to the experiment, as described above) were added to the HCPEpiC cultures, and cells were incubated for 1 to 24 hours as indicated in the figure legends. After incubation, cells were harvested using either Qiazol™ lysis reagent (for RNA extraction, QIAGEN, Germantown, MD, USA) or cell extraction buffer (for protein extraction, Invitrogen, Camarillo, CA, USA). Total RNA and protein was extracted from cells to evaluate mRNA expression and protein content, as described below.

Gram M., Sveinsdottir S., Cinthio M., Sveinsdottir K., Hansson S.R., Mörgelin M., Åkerström B, & Ley D. (2014). Extracellular hemoglobin - mediator of inflammation and cell death in the choroid plexus following preterm intraventricular hemorrhage. Journal of Neuroinflammation, 11, 200.

+ Open protocol

+ Expand

Culturing Primary Choroid Plexus Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human primary choroid plexus epithelial cells (HCPEpiC, ScienCell™ Research Laboratories, Carlsbad, CA, USA) were cultured in Epithelial Cell Medium (ScienCell) containing 2% fetal bovine serum (FBS, ScienCell), 1% Epithelial Cell Growth Supplement (ScienCell), and 1% Penicillin/Streptomycin solution (ScienCell) at 37 °C and 5% CO₂. For culturing these cells, the manufacturer’s protocol was followed. Cell density was set to 6000 cells per cm² and poly-L-lysine (ScienCell) -coated (2 µg/cm²) BioLite cell culture flasks (Thermo Scientific, Rochester, NY, USA) were used.

Fejes Z., Erdei J., Pócsi M., Takai J., Jeney V., Nagy A., Varga A., Bácsi A., Bognár L., Novák L., Kappelmayer J, & Nagy B J.r. (2020). Elevated Pro-Inflammatory Cell-Free MicroRNA Levels in Cerebrospinal Fluid of Premature Infants after Intraventricular Hemorrhage. International Journal of Molecular Sciences, 21(18), 6870.

+ Open protocol

+ Expand

Primary Human Choroid Plexus Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary human choroid plexus epithelial cells (HCPEpiC, ScienCell Research Laboratories, Carlsbad, CA, USA) were cultured in a special Epithelial Cell Medium (ScienCell) containing 2% fetal bovine serum (FBS, ScienCell), 1% Epithelial Cell Growth Supplement (ScienCell), and 1% Penicillin/Streptomycin solution (ScienCell) in a 5% CO₂ humidified atmosphere (95%) at 37 °C. For culturing these cells, we followed the manufacturer’s protocol. Cell density was set to 5000 cells per cm² and poly-L-lysine (ScienCell) coated (2 µg/cm²) BioLite cell culture flasks (Thermo Scientific, Rochester, NY, USA) were used. HCPEpiC cells (2 × 10⁵/well) at passages 4–6 were subcultured into BioLite 6-well plates (Thermo Scientific) and then treated with ex vivo IVH-III, IVH-IV, or non-IVH control CSF samples (10 v/v %) for 24 h. After treatment, cells were washed with Dulbecco’s Phosphate Buffered Saline (DPBS) solution (Lonza, Walkersville, MD, USA), then lysed in 1 mL TRI Reagent (Molecular Research Center, Cincinatti, OH, USA) and stored at −20 °C before RNA isolation.

Fejes Z., Pócsi M., Takai J., Erdei J., Tóth A., Balogh E., Rusznyák Á., Fenyvesi F., Nagy A., Kappelmayer J., Jeney V, & Nagy B J.r. (2021). Preterm Intraventricular Hemorrhage-Induced Inflammatory Response in Human Choroid Plexus Epithelial Cells. International Journal of Molecular Sciences, 22(16), 8648.

+ Open protocol

+ Expand

Isolation and Profiling of CNS Cell Types

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fresh peripheral blood mononuclear cells (PBMC) of two HD were obtained from Ficoll gradient-treated lymphocytapheresis samples. Monocytes, B-cells, CD4+ T cells, CD8+ T cells, natural killer cells, innate lymphoid cells, and myeloid dendritic cells were Fluorescence-activated cell sorted (FACS). Each purified cell subtype was cultured (1×10⁶ cells/ml) in serum-free X-VIVO 15 medium (Lonza, Walkersville, MD) with or without 10 μg/mL phorbol 12-myristate 13-acetate (PMA) and 1μM Ionomycin. Supernatants were collected after 48 hours and frozen until use.
Isolated primary human CNS cells or cell lines: human neurons (ScienCell, Carlsbad, CA), human astrocytes (ScienCell), human brain endothelial cell line (HCMEC/D3; provided by Pierre-Olivier Couraud, PhD, INSERM, France (13 (link))), human microglia cell line (CHME5; provided by Nazira El-Hage, PhD, Florida International University, USA), and human choroid plexus epithelial cells (hCPEpiC; ScienCell) were plated (10⁵ cells/ml; 10 ml/flask). Cells were treated with PBMC culture media (control) and an inflammatory mediators (supernatant from lipopolysaccharide- and CD3/CD28 beads-stimulated human PBMCs; 50% v/v). Oligodendrocytes were differentiated from the NIH approved human embryonic stem cell line RUES1 using published protocol (14 (link)). Cell-culture supernatants were collected after 24-hour incubation and frozen until use.

Barbour C., Kosa P., Komori M., Tanigawa M., Masvekar R., Wu T., Johnson K., Douvaras P., Fossati V., Herbst R., Wang Y., Tan K., Greenwood M, & Bielekova B. (2017). Molecular-based diagnosis of Multiple Sclerosis and its progressive stage. Annals of neurology, 82(5), 795-812.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!