Uv lamp

The samples used in the tests were the unmodified acrylic water-based paint (Un_Paint) and the self-disinfecting paints containing CLX in the concentration 0.15 g/L, TRP in the concentration of 6.0 g/L, and the mixture CLX+TRP in the concentration of 3.0 g/L. For antibacterial assessment, the paints were applied in 50 × 50 mm polymeric film square, according to ISO 22196 [18 ]. For cytotoxicity assessment, the paints were applied in a 10 × 10 mm polymeric film square. Before each test, every sample and parafilm were sterilized with UV-C light (294 nm) using a UV lamp from VWR (Radnor, PA, USA) for 15 min on each side.
For the tests by direct contact, samples of transparent polymeric film (W) and Copper (Cu²⁺) were used as negative and positive control of the surface, respectively.

The paints were prepared and characterized in the previous work by Querido et al. [6 (link)]. The samples used in the tests by direct contact and on extracts were the unmodified commercial water-based paint (Un_Paint) and the self-disinfecting paints containing TCS in the concentration 0.0012 g/L and ISB in the concentration of 1.2 g/L applied in a 10 × 10 mm polymeric film square. For the tests by direct contact, samples of transparent polymeric film (W) were used as the negative control of the surface and copper (Cu²⁺) was used as the positive control.
Before each test, every sample was sterilized with UV-C light (294 nm) using a UV lamp from VWR (Radnor, PA, USA) for 15 min on each side.