A neutral red (NR) assay modified from Borenfreund and Puerner (1984) was used to testfor cell viability. Briefly, cell lines were moved from a culture flask using 1X trypsin-EDTA. Then, RPMI supplemented with 10% FBS was added. The cells were counted using a hemocytometer and approximately 6,000 cells were seeded per well in a 96-well plate. The cells were cultured in completed RPMI 1640 medium at 37°C in a CO 2 incubator for 24 hr. All of the cell lines were treated with varying concentrations (30, 50, 70, 90, 110, 120 and 150 mM) of GA and GNPs-GA and incubated for an additional 24 hr. NR was added to the tested cells for 3 hr. Cell viability was detected at 540 nm using an RT-2100c micro plate reader (Rayto, China).
Rt 2100c microplate reader
Manufactured by Rayto
Sourced in China
The RT-2100C microplate reader is a compact and versatile instrument designed for absorbance-based microplate assays. It features a high-performance optical system and a user-friendly interface, enabling accurate and reliable measurements in a wide range of applications.
Automatically generated - may contain errors
13 protocols using rt 2100c microplate reader
1
NR Assay for Cell Viability
2
Secretin Level Measurement by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the identity was suggested by TagIdent, ELISA was used to detect the levels in sera. Secretin levels were measured in the serum samples using an ELISA 96-well plate for secretin (CEB075Hu, Cloud-Clone Corp., Houston, USA). The serum samples were diluted 1:1 with a 0.01 mol/L PBS (PH 7.0–7.2) according to the manufacturer’s instructions and analyzed on a RT-2100C micro plate reader (Rayto, USA) at a wavelength of 450 nm according to the manufacturer’s instructions.
3
Transwell Migration Assay for Cervical Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cervical cancer cell migration was further characterized through an established transwell migration assay29 (link), utilising 24-well hanging-inserts fitted with an 8 µm pore size membrane (Millicell Cell Culture Inserts Category No. MCEP24H48). 1 × 105 serum starved cells were seeded in triplicate in media containing 1% FBS, onto the apical surface of each hanging-insert and placed into wells containing 10% FBS. Following the addition of N. brasiliensis L3 antigen (10 µg) or 1X PBS, the plates were incubated for 24 hours and the lower surface of the insert fixed with methanol and stained with crystal violet. Excess crystal violet was washed off the insert using distilled water before release of the crystal violet stain into 50% acetic acid. The absorbance of this solution was then quantified at 595 nm using a Rayto RT-2100C Microplate Reader.
4
Serum Markers for Acute Appendicitis Diagnosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the serum markers were measured in the same serum sample concurrently. The serum levels of PTX3, WBC, IL-6 and hs-CRP were used data in estimating AA, and in estimating perforation in patients with AA.
Venous blood samples taken from the patient group and control group into hemogram test tubes containing EDTA were centrifuged at 15 minutes at 1000 x g within 30 minutes of collection, and then, they were stored at -40°C until the day that the analysis was performed. Pentraxin 3 (Human Pentraxin 3/ TSG-14 Immunoassay Kit) and IL-6 (Human IL-6 Quantikine Elisa Kit) were measured by RT-2100 C Microplate Reader (Rayto Life and Analytical Sciences Co., Ltd., China). CRP levels were measured by the nephelometric method in the RADIM Delta nephelometer (Radim Diagnostics, Pomezia, Italy). WBC count was measured by an automated hematology analyzer (Sysmex Corporation, Kobe, Japan). There were two reference standards of this study: (1) diagnosed AA, (2) perforated AA. AA was defined as inflammation of the vermiform appendix, and perforated AA was defined as a hole in the appendix or a presence of fecalith in the abdomen. AA and perforated AA were diagnosed with surgical exploration and pathologic examination.
Venous blood samples taken from the patient group and control group into hemogram test tubes containing EDTA were centrifuged at 15 minutes at 1000 x g within 30 minutes of collection, and then, they were stored at -40°C until the day that the analysis was performed. Pentraxin 3 (Human Pentraxin 3/ TSG-14 Immunoassay Kit) and IL-6 (Human IL-6 Quantikine Elisa Kit) were measured by RT-2100 C Microplate Reader (Rayto Life and Analytical Sciences Co., Ltd., China). CRP levels were measured by the nephelometric method in the RADIM Delta nephelometer (Radim Diagnostics, Pomezia, Italy). WBC count was measured by an automated hematology analyzer (Sysmex Corporation, Kobe, Japan). There were two reference standards of this study: (1) diagnosed AA, (2) perforated AA. AA was defined as inflammation of the vermiform appendix, and perforated AA was defined as a hole in the appendix or a presence of fecalith in the abdomen. AA and perforated AA were diagnosed with surgical exploration and pathologic examination.
5
Kidney Tissue Homogenization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were deeply anesthetized as described above. Immediately after the animals were euthanized their kidneys were isolated. The left kidney was used to measure NO metabolites and the right kidney was used for western blots. The renal cortex was dissected and homogenized at 3000 rpm in an appropriate buffer (in mmol/l: 250 sucrose, 1 EDTA, 0.1 PMSF and 10 Tris‐ClH), pH 7.6. Large tissue debris and nuclear fragments were removed by a low‐speed spin (1000 g, 10 min, 4°C). Protein concentration was measured using BCA™ Protein Assay Kit (Pierce, Rockford, IL). Absorbance for protein concentration measurement was read using an RT‐2100C microplate reader (Rayto, China) at 560 nm.
6
Serum Hormone Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples, obtained by cardiac puncture, were allowed to clot in plain bottles and centrifuged at 4000 rpm for 10 min using a centrifuge (800D manufactured by TOBITEK Allied Industries). Serum was subsequently harvested for testosterone, follicle stimulating hormone and luteinising hormone assays. Serum samples and ELISA hormone standards were prepared in standard ELISA microwells (Designed and manufactured by Monobind Inc., USA) following instructions in the ELISA kit operational Manual. The absorbance were read with ELISA machine (Rayto RT-2100C microplate reader). Serum hormonal assay were obtained from a plotted graph of absorbance versus concentration of hormone standards.
7
Serum Vitamin D Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stored serum were allowed to thaw at room temperature for the measurement of 25(OH)D using a sandwich enzyme linked-immunosorbent assay (ELISA) kits, following the manufacturer’s protocol. Absorbances were read at 450nm using a microplate reader (RT-2100C microplate reader, Rayto). The concentration of serum vitamin D in test samples were estimated from the standard curve plotted using the different standards with known concentrations. For the estimation of vitamin D, 14 samples were lost during storage, thus 148 stored samples were used for the estimation of vitamin D. Vitamin D concentrations < 20 ng/mL were considered as vitamin D deficiency.
8
Cell Proliferation Assay with CSE Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transfected and control A549 cells were treated with 10% CSE for 24 h. Cells from the different treatment groups were seeded in 96-well plates at a density of 3×103 cells/well. Cell proliferation was determined using the Cell Counting Kit-8 (Dojindo Molecular Technologies, Rockville, MD). At 0, 24, 48, and 72 h after inoculation, the medium was removed from the cells, 10 μL of CCK-8 reagent was added to each well, and the cells were incubated at 37 °C for 2 h. The optical density (OD) at 450 nm was determined using an RT-2100C microplate reader (Rayto Life and Analytical Sciences, Shenzhen, China). The OD value of each experimental group was equal to the measured OD value of the experimental group minus the OD value of the blank group.
9
Quantifying VEGF-C Levels in Tumor Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 100 mg tumor tissue from each sacrificed mouse from the various groups was ground with 200 ml cold PBS. Supernatants from the extract were subsequently collected and evaluated using an ELISA kit (USCN Life Science, Inc., Wuhan, China) to measure the protein concentration of VEGF-C according to the manufacturer's instructions. At the conclusion of the reaction, plates were read on the RT-2100C Microplate Reader (Rayto Life and Analytical Sciences Co., Ltd.). The results of the ELISA assay were expressed as pg/ml.
10
Kidney Outer Medulla Protein Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immediately after the animals were sacrificed, their kidneys were isolated and the outer medulla was dissected and homogenized at 3.000 rpm in an appropriate buffer (250 mmol/l sucrose, 1 mmol/l EDTA, 0.1 mmol/l PMSF and 10 mmol/l Tris-ClH), pH 7.6. Large tissue debris and nuclear fragments were removed by a low-speed spin (1000 g, 10 min, 4°C). Protein concentration was measured using BCA™ Protein Assay Kit (Pierce, Rockford, IL, USA). Absorbances for protein concentration measurements were read using an RT-2100C microplate reader (Rayto, China) at 560 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!