Ribozero kits

Total RNA was prepared as previously described with minor modifications (Hillier et al. 2009 (link)). Ribosomal subtraction was performed using Ribozero kits (Epicentre) according to the manufacturer's instructions. cDNA was generated, and RNA-seq libraries were prepared as previously described with minor modifications (Hillier et al. 2009 (link)). See Supplemental Methods for details.

The EDTA anticoagulation tube was used to draw 10 mL of peripheral venous blood in the morning of the next morning after fasting. The plasma was separated by high‐speed centrifugation and stored at −80°C in the refrigerator. At the same time, western blotting was used to detect the concentration of marker proteins on the surface of exosomes, so as to identify the extracted exosomes. Total RNAs were isolated from the exosomes in nine patients using the Trizol method, and the concentration and mass of the samples were determined using the Agilent 2100 Bioanalyzer, rRNAs were depleted using the Epicenter Ribo‐Zero kits, and then following the fragmentation of RNAs, first‐strand cDNAs were further synthesized from these RNAs via reverse transcription, a RNA gene library was constructed using PCR technique, and miRNAs were isolated and purified by high‐resolution polyacrylamide gel electrophoresis (PEAG) to form a miRNA library, which was then sequenced using the Illumina‐HiSeq4000 Sequencing Platform.