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2 protocols using hsa mir 181a 5p taqman microrna assay

1

Quantitative Analysis of miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
1) PureLink RNA Micro Scale Kit (Invitrogen, 12183016); 2) QIAshredder (50) (Qiagen, 79654); 3) 2- Mercaptoethanol (Sigma-Aldrich, M6250-10ML); 4) mirVana miRNA Isolation Kit, with phenol (Ambion, AM1560); 5) Ethanol absolute (200 Proof), Molecular Biology Grade (Fisher Scientific, 10644795); 6) Taqman MicroRNA Reverse Transcription kit (Applied Biosystems, 4366596); 7) Taqman Fast Advanced Mastermix (Applied Biosystems, 4444557); 8) Hsa-miR-181a-5p Taqman microRNA assay (ID: 000480) (Thermofisher Scientific, 4427975); 9) Hsa-miR-27a-3p Taqman microRNA assay (ID: 000408) (Thermofisher Scientific, 4427975); 10) RNU44 Human Taqman microRNA assay control miRNA (ID: 001094) (Thermofisher Scientific, 4427975); 11) Precision nanoScript2 Reverse Transcription Kit (Primerdesign, RT-NanoScript2); 12) Taqman Fast Universal PCR Master Mix (2×), no AmpErase UNG (Applied Biosystems, 4366072); 13) Reference gene assays with double-dye (Taqman style) probe for human ACTB and GAPDH genes (Primerdesign, HK-DD-hu-600); 14) Custom human real-time PCR assays with double-dye probe (Taqman style) 300rxn for CAV1 (Primerdesign, DD-hu-300)
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2

Quantitative Analysis of miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
1) PureLink RNA Micro Scale Kit (Invitrogen, 12183016); 2) QIAshredder (50) (Qiagen, 79654); 3) 2- Mercaptoethanol (Sigma-Aldrich, M6250-10ML); 4) mirVana miRNA Isolation Kit, with phenol (Ambion, AM1560); 5) Ethanol absolute (200 Proof), Molecular Biology Grade (Fisher Scientific, 10644795); 6) Taqman MicroRNA Reverse Transcription kit (Applied Biosystems, 4366596); 7) Taqman Fast Advanced Mastermix (Applied Biosystems, 4444557); 8) Hsa-miR-181a-5p Taqman microRNA assay (ID: 000480) (Thermofisher Scientific, 4427975); 9) Hsa-miR-27a-3p Taqman microRNA assay (ID: 000408) (Thermofisher Scientific, 4427975); 10) RNU44 Human Taqman microRNA assay control miRNA (ID: 001094) (Thermofisher Scientific, 4427975); 11) Precision nanoScript2 Reverse Transcription Kit (Primerdesign, RT-NanoScript2); 12) Taqman Fast Universal PCR Master Mix (2×), no AmpErase UNG (Applied Biosystems, 4366072); 13) Reference gene assays with double-dye (Taqman style) probe for human ACTB and GAPDH genes (Primerdesign, HK-DD-hu-600); 14) Custom human real-time PCR assays with double-dye probe (Taqman style) 300rxn for CAV1 (Primerdesign, DD-hu-300)
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