Si-XIST (small interference RNA of XIST), pc-XIST (XIST-overexpressing plasmid), pc-SOX4 (SOX4-overexpressing plasmid), and miR-140-5p mimic and inhibitor were constructed from GenePharma (Shanghai, China). Lipofectamine 2000 (Invitrogen, USA) was for transient transfection of constructs into cells with the manufacturer’s indication.
Pc xist
Manufactured by GenePharma
Sourced in China
Pc-XIST is a laboratory instrument designed for the quantitative analysis of gene expression. It utilizes real-time PCR technology to measure the level of specific RNA transcripts in biological samples. The core function of Pc-XIST is to provide accurate and reliable data on the expression of target genes, which is essential for various applications in the fields of genetics, genomics, and molecular biology.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
H460 ddp, by Thermo Fisher Scientific (1 mentions)
A549 ddp, by Thermo Fisher Scientific (1 mentions)
Mir 144 3p inhibitor, by GenePharma (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Inhibitor nc, by GenePharma (1 mentions)
2 protocols using pc xist
1
XIST and SOX4 Regulation by miR-140-5p
2
Investigating Cisplatin Resistance in NSCLC
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Cisplatin-resistant human NSCLC cell lines, H460/DDP and A549/DDP, were obtained from RiboBio Co. (Guangzhou, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA), penicillin and and streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37°C with 5% CO2. Culture medium was changed every 2 days.
The negative control (NC) mimic, microRNA (miR)-144-3p mimic, NC inhibitor and miR-144-3p inhibitor were purchased from GenePharma Co., Ltd. (Shanghai, China). Short-hairpin (sh)-NC, sh-XIST, pc-NC and pc-XIST were constructed by GenePharma Co., Ltd. (Shanghai, China). All vectors and oligonucleotides were transfected into H460/DDP and A549/DDP cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
The negative control (NC) mimic, microRNA (miR)-144-3p mimic, NC inhibitor and miR-144-3p inhibitor were purchased from GenePharma Co., Ltd. (Shanghai, China). Short-hairpin (sh)-NC, sh-XIST, pc-NC and pc-XIST were constructed by GenePharma Co., Ltd. (Shanghai, China). All vectors and oligonucleotides were transfected into H460/DDP and A549/DDP cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.
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