Eudragit® E100 (referred to EE100) was kindly provided by Evonik Industries, USA. Vaccinia virus was coated with EE100 by solvent evaporation. The polymer was dissolved in toluene with the addition of Span®60 as an emulsifier (20% w/v of EE100 in toluene supplemented with 5% w/v of Span®60). The virus was mixed into the organic phase at a ratio of 1:10 (virus:organic phase) by vigorous vortexing. This solution (200μL) was added dropwise into an external aqueous phase, consisting of 10mL of distilled water and 500μL of Tween 80 (5% v/v). The mixture was stirred for 24 hours at room temperature to allow the solvent to evaporate. During the evaporative process, the virus and polymer are co-precipitated in the droplets forming microparticles. The microparticles containing vaccinia virus were then collected and washed with phosphate buffer at a pH 7.0 and allowed to air dry.
Eudragit e100
Manufactured by Evonik
Sourced in Germany, India
Eudragit E100 is a pH-sensitive, anionic copolymer based on dimethylaminoethyl methacrylate, butyl methacrylate, and methyl methacrylate. It is a water-soluble polymer that dissolves in gastric fluid.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Eudragit epo, by Evonik (2 mentions)
Triethyl citrate tec, by Merck Group (1 mentions)
Dibutyl sebacate dbs, by Merck Group (1 mentions)
Polyvinyl pyrrolidone kollidon 30, by BASF (1 mentions)
Hexamethylenediamine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Dextran t40, by Cytiva (1 mentions)
Eudragit l100, by Evonik (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
No 40 filter paper, by Cytiva (1 mentions)
Potassium dihydrogen phosphate kh2po4, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
Ethanol, by Avantor (1 mentions)
Methanol, by RCI Labscan (1 mentions)
Madecassoside, by Merck Group (1 mentions)
Asiatic acid, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
Aluminum chloride hexahydrate, by Merck Group (1 mentions)
17 protocols using eudragit e100
1
Eudragit E100 Microencapsulation of Vaccinia Virus
2
Formulation and Characterization of Eudragit-Based Polymeric Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TT was a gift from Aurobindo Pharmaceuticals (India). Eudragit E 100, RS100, RL100, RSPO and RLPO were gift s from Evonik Röhm GmbH, Germany. EUDRAGIT ® polymers are copolymers derived from esters of acrylic and methacrylic acid whose physicochemical properties are determined by functional groups. In these studies, Eudragit E 100 is a cationic polymer with dimethylaminoethyl methacrylate as a functional group. Other Eudragit grades such as RS100, RL100, RSPO and RLPO are ethyl acrylate, methyl methacrylate copolymers with trimethyl-ammonioethylmethacrylate as diff erent ratios of the functional group. Polyvinyl pyrrolidone (Kollidon ® 30) was procured from the BASF Chemical Company, Germany. Dibutyl phthalate (DBP) was purchased from Qualigens Fine Chemicals, India. Triethyl citrate (TEC) was purchased from Merck Chemicals, Germany. Dibutyl Sebacate (DBS) was purchased from Sigma-Aldrich Chemie GmbH, Germany. All other chemicals used in the study were of analytical grade and were used as received.
3
Formulation and Characterization of Polymeric Nanocarriers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eudragit L100 (methacrylic acid-methyl methacrylate copolymer (1 :1)) powder (Mw= 125000 g/mol) and Eudragit E100 (dimethylaminoethyl methacrylate, butyl methacrylate, and methyl methacrylate tri-copolymer with a ratio of 2 :1 :1) pellets (Mw=47000 g/mol) were obtained from Evonik Röhm GmbH (Darmstadt, Germany). Sodium hydroxide was purchased from Sigma Aldrich (Sweden) and hydrochloric acid (35%) from VWR Chemicals (France). Sodium chloride was obtained from Laurylab (Brindas, France). Branched polyethylenimine (PEI, Mw=25000 g/mol, Mn∼10000) was purchased from Aldrich Chemistry (Germany), polyvinyl alcohol (Mw=200000 g/mol) from Merck KGaA (Darmstadt, Germany) and polyacrylic acid 40% wt. (Mw=30000 g/mol) from Aldrich Chemical Company (Milwaukee, USA). Dextran 40 (Mw=40000 g/mol) was a product of AppliChem GmbH (Darmstadt, Germany) and Pluronic F68 was purchased from Molekula Group (Shaftesbury, Dorset, Great Britain). Amino-dextran was prepared according to Mouaziz et al. [35] (link) and using hexamethylenediamine from Merck (Germany) and Dextran-T40 from Amersham Biosciences (Germany)(Table 1).
4
Polymeric Nanoparticle Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MZB (Purity 99.8%) was a gift received from Siza International (pvt) Ltd. Lahore, Pakistan. Eudragit E 100 (Evonik, Germany) (Purity 99%). Polycaprolactone, (Mw ~14,000) (Sigma Aldrich). PVA (Purity 98%) (Merck, Germany). Dicholoromethane (DCM) (Merck, Germany). Sodium hydroxide (NaOH) (Merck, Germany). Potassium bromide (KBr) of FTIR grade (Fischer Scientific UK). Potassium dihydrogen phosphate (KH2PO4) (Merck, Germany). Hydrochloric acid (HCl) (RDH). Filter paper (Whatman filter paper no. 40) and double distilled water (DDW) was used throughout the studies collected from laboratory.
5
Polymer Film-Forming Formulations for Drug Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ABIL T Quat 60 was kindly provided as a sample by Evonik Nutrition & Care GmbH (Essen, Germany), and formulated in solutions of different concentrations (5%, 10%, 15%, 20%, 25%, and 30%) in 95% ethanol (100% ethanol, VWR, Fontenay-sous-Bois, France, with 5% distilled water). Eudragit E100, in granules, was received as a sample from Evonik Röhm GmbH (Darmstadt, Germany). Film-forming formulations of the latter polymer were made by dissolving the Eudragit (5%, 10%, 15%, 20%, 25%, and 30%) and the plasticizer triethyl citrate (20% w/w of the dry polymer; ≥98.0%) in 95% ethanol [22 (link),23 ]. The formulations were stirred for 24 h until clear solutions were formed.
6
Asiatic Acid and Madecassoside Formulation Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reference compounds of asiatic acid (³ 97 %) and madecassoside (³ 95 %) were obtained from Sigma-Aldrich Co. (St. Louis, USA). Madecassic acid (³ 98 %) and asiaticoside (³ 98 %) were obtained from Chengdu Biopurity Phytochemicals Ltd. (Chengdu, China), and Fluka Chemie GmbH (Switzerland), respectively. HP-b-CD was obtained from ISP Pharmaceuticals (Wayne, USA). Polyethylene glycol 400 (PEG 400) was procured from Srichand United Dispensary (Thailand). Eudragit ® E100 (amino methacrylate copolymer) was obtained from Evonik (Germany). Kolidon ® VA 64 (copovidone) was obtained from BASF (Germany). Glycerine was supplied by the P.C. Drug Center (Thailand). Ethanol (absolute) was obtained from V.S. Chem. House (Thailand). Petroleum ether (boiling point 60 °C) and methanol were purchased from RCI Labscan (Thailand). All other chemicals were of pharmaceutical grade and were used as received.
7
Colloidal Silica Nanoparticle Functionalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The colloidal silica nanoparticle (30.4 weight/volume [wt/v]% in deionized [DI] water) suspension with 20 nm (Cat No: NPS-SOL 20) and 100 nm (Cat No: NPS-SOL 100) diameter sizes were purchased from E&B Nanotech Co, Ltd (Ausan, Gyeonggi-do, South Korea) and are abbreviated as SiO2EN20(−) and SiO2EN100(−), respectively. The EUDRAGIT® E-100 was purchased from Evonik Industries (Essen, Germany). The aluminum chloride hexahydrate (Cat No: 237078), ethylenediamine ([EDA] Cat No: 240729), tris(2-aminoethyl)amine ([TAEA] Cat No: 225630), 3-aminopropyltriethoxysilane ([APTES] Cat No: A3648), L-serine ([L-ser] Cat No: S4311), L-histidine ([L-his] Cat No: H8000), and L-arginine ([L-arg] Cat No: A5006) were purchased from Sigma Aldrich Co., LLC. Hydrochloric acid ([HCl] Cat No: 1129) and sodium hydroxide ([NaOH] Cat No: 7570-4400) were supplied by DUKSAN Co., Ltd (South Korea) and Daejung Chemicals and Metals Co., Ltd (South Korea), respectively.
8
Diclofenac Sodium Extended-Release Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diclofenac sodium obtained from Darwin Formulations Pvt. Limited, Vijayawada as gift sample, Xanthan gum purchased from Sigma Aldrich, Eudragit E100 purchased from Evonik Degussa, Mumbai, Polyvinyl pyrrolidone K-30 purchased from Loba chime, Mumbai, Magnesium stearate purchased from Reachem labs, New Delhi. All other chemicals used were of analytical grade.
9
Formulation and Characterization of Polymeric Colloidal Carriers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Branched-polyethyleneimine (PEI, MW = 25 kDa), polyoxyethylene (20) sorbitan monooleate (Tween 80), 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB2−), N-acetylcysteine (NAC), macrogolglycerol ricinoleate (Kolliphor EL), fluorescein diacetate (FDA), sorbitan trioleate (Span 85), and dipropylene glycol were purchased from Sigma-Aldrich (Vienna, Austria). Evonik Röhm GmbH (Darmstadt, Germany) gifted the methacrylate-copolymers Eudragit® E 100 (poly(butyl methacrylate-co-(2-demethylaminoethyl) methacrylate-co-methyl methacrylate), 1:2:1) and Eudragit® RS 100 (trimethylammonioethyl methacrylate, methyl methacrylate, and ethyl acrylate, 0.1:2:1). 2-n-Octyl-1-dodecanol as an oil phase was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The remaining materials and solvents were of analytical grade and purchased from commercial sources.
10
Carvedilol Formulation Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials used in the presented study are: carvedilol (CARV, Ph. Eur. 9.0) and following (co)polymers: polyvinylpyrrolidone(PVP, Kollidon ® 30, BASF, Germany), hydroxypropyl methylcellulose (HPMC, Methocel ® E5 LV, Dow ® , USA), polyvinyl acetate and polyvinyl caprolactam copolymer (Soluplus ® , BASF, Germany) and poly-(N-dimethylaminoethyl methacrylateco-methyl methacrylate-co-butyl methacrylate (Eudragit ® E-100, Evonik, Germany). Commercial carbon dioxide (purity 99%) was supplied by Messer-Tehnogas (Serbia).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!