ML323 was from Selleck Chemicals. CHX was purchased from Apexbio Company. LPS (Escherichia coli, 055:B5) and poly(I:C) were from Sigma-Aldrich. ISD was purchased from Invivogen. IFN-β was from Sino Biological Inc. The concentrations of agonists or stimuli were used as follows: 100 ng/ml LPS, 10 µg/ml poly(I:C), 10 µg/ml ISD, and 20 ng/ml IFN-β. Anti-USP1 (8033), anti-TBK1 (3054), anti-IRF3 (4302), anti–p-IRF3(Ser396; 4947), and anti–p-STAT1 (9167) were from Cell Signaling Technology. Anti-UAF1 (ab122473) and anti-V5 (ab9116) were from Abcam. Anti-Flag (F1804), anti-HA (H3663), anti-Myc (M4439), and anti–VSV-G (V4888) were from Sigma. Anti-USP12 (12608–1-AP) and anti-USP46 (13502–1-AP) were from Proteintech. Anti–Lamin A/B (BS1446) was from Bioworld Technology. Anti–β-actin (sc-81178) and protein G agarose (sc-2002) used for immunoprecipitation and horseradish peroxidase–conjugated secondary antibodies were from Santa Cruz Biotechnology. SeV was purchased from the China Center for Type Culture Collection, and the multiplicity of infection used was 1.
Anti β actin sc 81178
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti–β-actin (sc-81178) is a mouse monoclonal antibody that recognizes the β-actin isoform. It is a primary antibody used for the detection and quantification of β-actin in various samples and applications.
Automatically generated - may contain errors
Lab products found in correlation
Lps escherichia coli 055 b5, by Merck Group (1 mentions)
Ifn β, by Sino Biological (1 mentions)
Anti v5 ab9116, by Abcam (1 mentions)
Ml323, by Selleck Chemicals (1 mentions)
Lipopolysaccharides (lps), by Sino Biological (1 mentions)
Anti cd31 antibody dia 310, by Dianova (1 mentions)
Peroxidase labeled anti rabbit antibody nif 824, by GE Healthcare (1 mentions)
Peroxidase labeled anti mouse antibody nif 825, by GE Healthcare (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Fungizone, by Thermo Fisher Scientific (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Erastin, by Merck Group (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Mito tempo, by Enzo Life Sciences (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Anti vimentin 5741, by Cell Signaling Technology (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
Bx795 tlrl bx7, by InvivoGen (1 mentions)
6 protocols using anti β actin sc 81178
1
Regulation of IFN Signaling by USP1
2
Antibody Procurement for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The anti-β-actin (sc81178) and the anti-nuclear factor erythroid 2-related factor (Nrf)2 antibodies (sc-365,949) were purchased from Santa Cruz Biotechnology (Dallas, Texas). The anti-CD31 antibody (DIA310) was purchased from Dianova (Hamburg, Germany). The anti-somatostatin (Ab30788), anti-insulin (Ab181547) and anti-glucagon (Ab92587) antibodies were purchased from Abcam (Cambridge, UK). The anti-heme oxygenase (HO)-1 antibody (ADISPA895F) was purchased from ENZO (New York, USA). The peroxidase-labeled anti-rabbit antibody (NIF 824) and the peroxidase-labeled anti-mouse antibody (NIF 825) were purchased from GE Healthcare (Freiburg, Germany). The anti-SOD1 (TA321133) and anti-SOD2 (TA321189) antibodies were purchased from OriGene (Rockville, USA).
3
Fibroblast Cultivation and Autophagy Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary human dermal neonatal fibroblast (HDFn) and foreskin adult fibroblast (NuFF) cells were purchased from ATCC (Manassas, VA, USA), and cultured in DMEM medium (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, USA) and 1X Antibiotic-Antimycotic (100 U/mL penicillin, 100 μg/mL streptomycin, Fungizone® 0.25 μg/mL, Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with 5% CO2. Mito-TEMPO was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Erastin, 3-MA, chloroquine, deferipron, and NAC were from Sigma-aldrich (St. Louis, MO, USA). The following antibodies were purchased by Santa Cruz Biotechnology (Santa Cruz, CA, USA): anti-APG5 (sc-33210), anti-BECN1 (sc-11427), anti-LC3B (L7543), and anti-β-actin (sc-81178).
4
Immunohistochemistry and Western Blotting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WB and Immunohistochemistry (IHC) were performed as described in the previous studies (Huai et al., 2016 (link); Yang et al., 2021 (link)). Anti-Vimentin 5741 was from Cell Signaling Technology. Anti–CILP (ab192881) was from Abcam. Anti–CILP (HPA003195) was from Atlas. Anti-Flag (F1804) was from Sigma. Anti–β-actin (sc-81178) and horseradish peroxidase-conjugated secondary antibodies were from Santa Cruz Biotechnology.
5
Investigating Innate Immune Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-IFI16 (SAB1408587), anti-DAI (PRS4401), and anti-cGAS (SAB3500110) antibodies were purchased from Sigma (St. Louis, MS, USA). Anti-phospho-IRF3 (number 4947) and anti-ISG15 (number 2743) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin (sc-81178), anti-IRF3 (sc-9082), and anti-Mx1 (sc-50509) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-TLR3 (ab137722), anti-TLR7 (ab45371), anti-TLR8 (ab24185), anti-TLR9 (ab12121), anti-MDA5 (ab69983), anti-RIG-I (ab45428), anti-OAS1 (ab86343), and anti-Pol III (ab22236) antibodies were purchased from Abcam (Cambridge, UK). Poly(I:C)/Lyovec™ (tlrl-piclv), HSV60/Lyovec (tlrl-hsv60c), and BX795 (tlrl-bx7) were purchased from InvivoGen (San Diego, CA, USA). Small interfering RNA (siRNA) targeting RIG-I (sc-61480), TLR3 (sc036685), and IFI16 (sc-166504) and control siRNA (sc-37007) targeting a scrambled sequence were purchased from Santa Cruz Biotechnology.
6
MPTP-induced Parkinson's Disease Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sterile acupuncture needles (0.16 × 7 mm) were obtained from Beijing Zhongyan Taihe Medical Equipment Corporation (Beijing, China). SEMZ-II electronic acupuncture device was purchased from Suzhou Medical Supplies Factory Ltd (Suzhou, China). The open field test device was obtained from Wuhan Yihong Technology Corporation (Wuhan, China). FashHS SYBR QPCR mixture and AMeasy 1st Strand cDNA synthesis kit were purchased from Summer Bio (Beijing, China). Primary antibodies were obtained from the following companies: anti-NLRP3 (#15101, 1) from Cell Signaling Technology (Danvers, MA, USA); anti-ASC (ES1715) from Elk Biotechnology (Wuhan, China); anti-Tyrosine Hydroxylase (A0028), anti-Caspase-1 (A0964), anti-BDNF (A18129), and anti-α-synuclein (A13354) from Abclonal Corporation (Wuhan, China); anti-β-actin (sc-81178) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other chemicals and reagents were of analytical purity grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!