Uv winlab

The UV/Vis measurements were performed on a Lambda 1050 UV/Vis/NIR spectrometer form Perkin Elmer (Waltham, MA, USA). The software used to record the measured spectra was Perkin Elmer UVWinLab (Waltham, MA, USA). For the measurements, the loaded coated particles were dissolved in ALF to facilitate the release of all their cargo. 1 mL of this solution was then diluted with 2 mL H₂O yielding a total of 3 mL and measured in a quartz cuvette. For the pure drugs, 1 mL of the aqueous solution was diluted with 1 mL H₂O and 1 mL ALF.

Ultraviolet–visible (UV–vis) absorption spectra were recorded on a Perkin Elmer Lambda 900 UV/Vis/NIR spectrometer using the software Perkin Elmer UV Winlab (version 6.04.0738). Steady state photoluminescence spectra were recorded with a HORIBA Jobin‐Yvon Fluorolog 3–22 Tau‐3 using a photomultiplier tube (PMT) as detector.

Photodegradation tests were performed using a light cabinet Suntest CPS+ (Heraeus, Milan, Italy) according to the ICH guidelines [31 ]. A Xenon lamp inside the cabinet and an electronic device were provided to measure and control the irradiation power. The system was also able to check the temperature inside the cabinet. The samples were irradiated according to the ID65 standard of the ICH rules.
The photodegradation experiments were monitored by UV analysis at sequential exposure times. UV spectra were recorded by a Perkin-Elmer Lambda 40P spectrophotometer (Artisan Technology Group, Mercury Drive Champaign, IL, USA) under the following conditions: λ range 200–450 nm, scan rate 1 nm s⁻¹; time response 1 s; spectral band 1 nm.
Spectral data were elaborated using a multivariate approach. UV spectra were acquired by UV WinLab^® (Perkin-Elmer, Boston, MA, USA) and the multivariate analysis was performed using the Matlab^® computer environment software (Mathwork Inc., version 7, Natick, MA, USA).

The absorption spectra of free 5-MBT, the liposomal form of 5-MBT, and of the liposome-drug-albumin systems were recorded with the Lambda Bio 40 spectrometer (Perkin Elmer, Walthman, MA, USA) equipped with a PTP-1 Peltier System (Perkin Elmer, Walthman, MA, USA) automatic temperature controller. The temperature was maintained at 37 ± 0.1 °C. All the spectra were acquired after equilibration of the samples with the automatic temperature controller. The spectral analysis was performed using UV WinLab Perkin Elmer Software.