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The PAMC82 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for core functional purposes, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.

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2 protocols using pamc82

1

Cell Lines for Cancer Research

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Human gastric cancer cell lines PAMC82 and AZ521 were obtained from Beijing Tumor Hospital (Beijing, China) and KCLB (Korean Cell Line Bank, Seoul, Korea), respectively. Human breast cancer cell line T47D was obtained from ATCC (American Type Culture Collection, Rochville, MD, USA). SNU-638 cells were obtained from Korean Cell Line Bank. T47D cell line was cultured in Dulbecco's Modified Eagle's Medium (Invitrogen, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Invitrogen), 2 mmol/l L-Glutamine (L-Glu, Fisher Scientific, Ottama, ON, Canada). PAMC82, SNU638 and AZ521 cells were maintained in RPMI 1640 (Invitrogen) supplemented with 10% fetal bovine serum and 2 mmol/l L-Glutamine. All cells were maintained in a humidified incubator with 5% CO2 at 37 °C. Antibodies used in this study were obtained from the following sources: TNIK (GeneTex, Irvine, CA, USA, Cat. GTX13141), phospho-Akt (S473) (Dako, Produktionsvej, Glostrup, Denmark Cat.M3628), phospho-Erk1/2 (Thr202/Tyr204) (Cell Signaling Technologies, Danvers, MA, USA, Cat. 4370), AKT (Cell Signaling Technologies, Cat. 9272), LC3 (Novus Biologics, Littleton, CO, USA, Cat.NB100-2220), and GAPDH (glyceraldehyde 3-phosphate dehydrogenase; Cell Signaling Technologies, Cat. 2118).
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2

GC Cell Line Culture and Ethics

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The human GC cell lines PAMC-82 and SNU16 were obtained from the Chinese Academy of Sciences. The PAMC-82 cell line was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum. The SNU16 cell line was cultured in RPMI-1640 medium. All of the cell lines were confirmed to be free of mycoplasma contamination after testing with the kit from Shanghai Yise Medical Technology (MD001). The commercial tissue microarrays were constructed by Shanghai Biochip Co. Ltd. The study was approved by the medical ethics committee of Cancer Hospital, Chinese Academy of Medical Sciences (Beijing, China) (Ethical approval number: NCC1999 G-003).
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