R-warfarin, S-warfarin, racemic warfarin, warfarin metabolites (4’-, 6-, 7-, 8- and 10-OH-warfarin), and warfarin-d5 (deuterated phenyl ring; used as internal standard [IS]) were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Ammonium acetate and dimethylsulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Optima-grade water, acetonitrile, methanol, and acetic acid were obtained from Fisher Scientific (Pittsburgh, PA, USA). Blank human plasma (collected in K2-EDTA tubes) was purchased from Innovative Research (Novi, MI, USA). Recombinant human CYP3A4 and CYP2C9 Supersomes™, microsomes prepared from baculovirus-infected insect cells expressing human CYP enzymes and NADPH-cytochrome P450 reductase, were purchased from BD Gentest (Woburn, MA, USA).
S warfarin
Manufactured by LGC
Sourced in Canada
S-warfarin is a laboratory standard used for analytical and research purposes. It serves as a reference material to support the identification, quantification, and quality control of S-warfarin in various analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
R warfarin, by LGC (4 mentions)
Formic acid, by Merck Group (3 mentions)
Warfarin sodium, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Ko143, by Enzo Life Sciences (2 mentions)
K2 edta tubes, by Innovative Research (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Optima grade water, by Thermo Fisher Scientific (1 mentions)
Blank human plasma, by Innovative Research (1 mentions)
Hank s buffered salt solution hbss, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (1 mentions)
Warfarin sodium, by J&K Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
5 protocols using s warfarin
1
Quantitative Analysis of Warfarin and Metabolites
2
Warfarin-Resveratrol Interaction Assay
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(±)Warfarin sodium (purity 98%), caffeic acid (purity 98%), formic acid, 3-(40,50-dimethylthiazol-20-yl)-2,5-diphenyltetrazolium bromide (MTT) and sodium dodecyl sulfate were purchased from Sigma-Aldrich Chemical Co. (MO, U.S.A.). R-warfarin (purity 98%) and S-warfarin (purity 98%) were obtained from Toronto Research Chemicals, Inc. (Ontario, Canada). Ko143 (purity 96%) was obtained from Enzo Life Sciences, Inc. (NY, U.S.A) Fetal bovine serum, Dulbecco’s modified Eagle medium, penicillin–streptomycin-glutamine and Hank’s buffered salt solution (HBSS) were obtained from Invitrogen (CA, U.S.A). Resveratrol (purity > 99%) was obtained from TCI (Tokyo, Japan). Trans-resveratrol capsules were purchased from Doctor’s BEST (CA, U.S.A.). Acetonitrile and methanol of LC–MS grade were obtained from J.T. Baker Inc. (PA, U.S.A.).
3
Quantification of Warfarin and Metabolites
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S-(-)-warfarin (purity, 97%), R-(+)-warfarin (purity, 98%), (S)-7-hydroxy warfarin (purity, 98%), (R)-7-hydroxy warfarin (purity, 98%), (S)-10-hydroxy warfarin (purity, 97%), (R)-10-hydroxy warfarin (purity, 97%), and 3′-, 4′-, 6-, and 8-hydroxy warfarin (purity, 98%) were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Warfarin sodium (purity, 98%) and diclofenac sodium (purity, ≥99%) were purchased from J&K Scientific Ltd. (Beijing, China). LC-MS grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany). Formic acid (FA) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultra-pure water was purified by a Milli-QTM system (Millipore, Bedford, MA, USA).
4
Chromatographic Isolation and Characterization of Bioactive Compounds
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(±) Warfarin sodium, caffeic acid, rutin, myricetin, quercetin, kaempferol, protocatechuic acid, isorhamnetin, 6,7-dihydroxycoumarinand formic acid were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). S-warfarin and R-warfarin were purchased from Toronto Research Chemicals, Inc. (North York, ON, Canada). Scopolatin was obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). Cyanidin chloride was purchased from ChromaDex, Inc. (Irvine, CA, USA). Acetonitrile and LC/MS grade methanol were obtained from J.T. Baker Inc. (Center Valley, PA, USA). Mitoxantrone (MXR) and Ko143 were obtained from Enzo Life Sciences, Inc. (New York, NY, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and trypsin/EDTA were obtained from Invitrogen (Carlsbad, CA, USA). Milli-Q plus water (Billerica, MA, USA) was used for all preparations.
5
Metabolic profiling of human liver
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Chemicals Loperamide hydrochloride, oxethazaine, pa-* To whom correspondence should be addressed. e-mail: marisuke@med.showa-u.ac.jp paverine hydrochloride, pirenzepine hydrochloride hydrate, and trimebutine maleate were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Bufuralol and 1′-hydroxybufuralol were obtained from Gentest Co. (Waburn, MA, U.S.A.). S-Warfarin, S-mephenytoin, and midazolam were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada), ENZO Life Sciences Inc. (New York, NY, U.S.A.), and Nippon Roche K.K. Inc. (Osaka, Japan), respectively. 7-Hydroxywarfarin, 1′-hydroxymidazolam and 4-hydroxymephenytoin were obtained from Daiichi Pure Chemicals Co., Ltd. (Tokyo, Japan). Ethoxyresorufin and resorfin were provided by Sigma-Aldrich Co. (St. Louis, MO, U.S.A.). All other chemicals and reagents used were of the highest, commercially available quality.
Human Liver Samples Human liver microsomes (a pooled fraction from 10 patients) were obtained from the nonprofit Human and Animal Bridging Research Organization (Chiba, Japan). The study protocol was approved by the Ethics Committee of Showa University School of Medicine.
Human Liver Samples Human liver microsomes (a pooled fraction from 10 patients) were obtained from the nonprofit Human and Animal Bridging Research Organization (Chiba, Japan). The study protocol was approved by the Ethics Committee of Showa University School of Medicine.
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