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Ab1206

Manufactured by Abcam
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Ab1206 is a primary antibody produced by Abcam. It is designed for use in various immunoassay techniques.

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Lab products found in correlation

Ab37406, by Abcam (2 mentions) Pe conjugated isotype control, by BioLegend (2 mentions) Ab81051, by Abcam (2 mentions) Mouse iggk bp hrp, by Santa Cruz Biotechnology (1 mentions) N succinimidyl 3 2 pyridyldithio propionate spdp, by Thermo Fisher Scientific (1 mentions) Human pd l1 simplestep elisa kit, by Abcam (1 mentions) Imfinzi, by AstraZeneca (1 mentions) Cetuximab, by Merck Group (1 mentions) Ab18184, by Abcam (1 mentions) Goat anti mouse igg apc, by BD (1 mentions) Sc 516102, by Santa Cruz Biotechnology (1 mentions)

5 protocols using ab1206

1

Quantifying Cell Surface and Intracellular Protein Expression

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Cells transfected with CD4 MESA or N-terminal 6xHis-tagged mCherry or dTomato MESA (no reporter plasmid) were harvested as previously described, fixed in 2% paraformaldehyde in PBS, and either left intact for surface labeling or permeabilized in PWB buffer (0.5% saponin, 0.2% BSA in PBS) for whole-cell labeling. Cells transfected with 6x His-tagged constructs were incubated with fluorescein isothiocyanate (FITC)-conjugated rabbit polyclonal antibodies against the 6x His tag (ab1206 from Abcam), or a FITC-conjugated rabbit IgG isotype control (ab37406 from Abcam) to control for nonspecific binding. FITC expression was quantified by flow cytometry and analyzed as previously described, with mCherry or dTomato on MESA chains serving as the transfection control for gating. CD4 construct-transfected cells were incubated with phycoerythrin (PE) conjugated rat anti-mouse CD4 antibodies (#100408 from BioLegend) or PE-conjugated isotype control (#400607 from BioLegend).
Daringer N.M., Dudek R.M., Schwarz K.A, & Leonard J.N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synthetic Biology, 3(12), 892-902.
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2

Quantifying CD4 and 6xHis-tagged Protein Expression

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Cells transfected with CD4 MESA or N-terminal
6xHis-tagged mCherry or dTomato MESA (no reporter plasmid) were harvested
as previously described, fixed in 2% paraformaldehyde in PBS, and
either left intact for surface labeling or permeabilized in PWB buffer
(0.5% saponin, 0.2% BSA in PBS) for whole-cell labeling. Cells transfected
with 6xHis-tagged constructs were incubated with fluorescein isothiocyanate
(FITC)-conjugated rabbit polyclonal antibodies against the 6xHis tag
(ab1206 from Abcam), or a FITC-conjugated rabbit IgG isotype control
(ab37406 from Abcam) to control for nonspecific binding. FITC expression
was quantified by flow cytometry and analyzed as previously described,
with mCherry or dTomato on MESA chains serving as the transfection
control for gating. CD4 construct-transfected cells were incubated
with phycoerythrin (PE) conjugated rat antimouse CD4 antibodies (#100408
from BioLegend) or PE-conjugated isotype control (#400607 from BioLegend).
Daringer N.M., Dudek R.M., Schwarz K.A, & Leonard J.N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synthetic Biology, 3(12), 892-902.
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3

Durvalumab Binding Assay Protocol

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Durvalumab (Imfinzi; AstraZeneca) was obtained from Universal Oncology Center. The FITC‐anti‐human PD‐L1 (ab224074; Abcam), goat polyclonal Ab to human IgG (FITC) (ab81051; Abcam), Rb polyclonal Ab to 6× His‐tag (FITC) (ab1206; Abcam), mouse‐IgGK BP‐HRP (sc‐516102; Santa Cruz Biotechnology). N‐succinimidyl 3‐(2‐pyridyldithio)propionate (SPDP, 21857; Thermo Fisher Scientific). Human PD‐L1 SimpleStep ELISA Kit (ab214565; Abcam).
Zhang C., Xiong J., Lan Y., Wu J., Wang C., Huang Z., Lin J, & Xie J. (2020). Novel cucurmosin‐based immunotoxin targeting programmed cell death 1‐ligand 1 with high potency against human tumor in vitro and in vivo. Cancer Science, 111(9), 3184-3194.
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4

Affibody Binding Kinetics Evaluation

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Detached MS1-Thy1 and MS1-CD276 cells were washed and individually labelled with varying concentrations of purified affibody for 25 minutes at 4 °C with rotation unless additional time was needed to approach binding equilibrium. Cells were pelleted at 500g for 3 minutes and washed with 1 mL of ice cold PBSACM prior to labelling with 50 uL anti-His6 FITC conjugate (Abcam ab1206; 10 μg/mL) for 25 minutes at 4 °C. Cells were again pelleted and washed with 1 mL of ice cold PBSACM. Fluorescence was analyzed using an Accuri C6. The dissociation constant was calculated by non-linear least squares regression using a 1:1 binding model.
Stern L.A., Lown P.S., Kobe A.C., Abou-Elkacem L., Willmann J.K, & Hackel B.J. (2019). Cellular-Based Selections Aid Yeast-Display Discovery of Genuine Cell-Binding Ligands: Targeting Oncology Vascular Biomarker CD276. ACS combinatorial science, 21(3), 207-222.
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5

Cetuximab Antibody Production Protocol

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Cetuximab was obtained from Merck. Goat pAb to human IgG (FITC) (ab81051; Abcam), Rb pAb to 6X His-tag® (FITC) (ab1206; Abcam), mouse-IgGK BP-HRP (sc-516102; Santa Cruz Biotechnology), and mouse anti-6X His Tag® antibody (ab18184; Abcam) were used in the present study. Goat-anti-mouse IgG-APC (550826; BD Bioscience), and FITC conjugate goat anti-mouse IgG (AMS.ASS1105-1000; Boster Biological Technology) were also used. Mouse anti-CUS antibody, 1G9, was produced by our laboratory. Escherichia coli strain BL21 (DE3), plasmid pET32a (Sangon Biotech), and Ni-NTA Sepharose FF (GE Healthcare) were utilized.
Zhang C., Cai Y., Dai X., Wu J., Lan Y., Zhang H., Lu M., Liu J, & Xie J. (2020). Novel EGFR-bispecific recombinant immunotoxin based on cucurmosin shows potent anti-tumor efficiency in vitro. Oncology Reports, 45(2), 493-500.
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