DSS salt (colitis grade, molecular weight: 36,000–50,000 Daltons) was purchased from MP Biomedicals (Ohio, USA). The hematoxylin-eosin (H&E) staining solution was purchased from Sigma (St. Louis, MO, USA). Trizol reagent was obtained from Invitrogen (Carlsbad, USA), and Primescript™ RT reagent kits and SYBR® Premix Ex Taq™ II kits were acquired from Takara Bio Inc. (Kusatsu, Japan). Antibodies including p44/42 MAPK (Erk1/2), phospho-p44/42 MAPK (Thr202/Tyr204), p38 MAPK, phospho-p38 MAPK (Thr180/Tyr182), SAPK/JNK, phospho-SAPK/JNK (Thr183/Tyr185), Akt, phospho-Akt (Thr450), and beta-Actin were purchased from Cell Signaling Technology (MA, USA).
Dss salt colitis grade
Manufactured by MP Biomedicals
Sourced in United States
DSS salt (colitis grade) is a laboratory reagent used for research purposes. It is a commonly used chemical compound in the induction of experimental colitis models. The core function of this product is to serve as a tool for researchers studying inflammatory bowel diseases and related conditions.
Automatically generated - may contain errors
Lab products found in correlation
Phospho akt thr450, by Cell Signaling Technology (1 mentions)
Hematoxylin eosin h e staining solution, by Merck Group (1 mentions)
Sybr premix ex taq 2 kit, by Takara Bio (1 mentions)
Sapk jnk, by Cell Signaling Technology (1 mentions)
Phospho p38 mapk thr180 tyr182, by Cell Signaling Technology (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
2 protocols using dss salt colitis grade
1
Dextran Sulfate Sodium-Induced Colitis Protocol
2
Acute Colitis Induction in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DSS salt (colitis grade; molecular weight, 36,000–50,000 daltons; MP Biomedicals, Irvine, CA) was given to mice as a 2% filtered solution in drinking water, ad libitum, to induce acute colitis. DSS-containing water was refreshed once every 2–3 days. After 5 days of DSS administration, mice were allowed to recover with normal drinking water. The body weight and survival of each mouse was monitored at the indicated time points. No more than 5 mice were kept in 1 cage.
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