These analyses were carried out as previously described7 (link). The following antibodies were used in this study: SALL4 antibody (Abcam, Cambridge, MA); HBc antibody (Dako, Carpinteria, CA); RNA polymerase II antibody (Santa cruz biotechnology, Dallas, Texas); BRG1 antibody (Abcam, Cambridge, MA); STAT3 antibody (Santa Cruz biotechnology, Dallas, Texas); OCT4 antibody (Abcam, Cambridge, MA); H3K27me3 antibody (Abcam, Cambridge, MA); Histone 3 antibody (Active Motif, Carlsbad, CA). ChIP primer sequences listed in Supplementary Table 1 .
Brg1 antibody
Manufactured by Abcam
Sourced in United Kingdom
The BRG1 antibody is a research tool used for the detection and analysis of the BRG1 protein, which is a subunit of the SWI/SNF chromatin remodeling complex. This antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the BRG1 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Hbc antibody, by Agilent Technologies (2 mentions)
Rna polymerase 2 antibody, by Santa Cruz Biotechnology (2 mentions)
Histone 3 antibody, by Active Motif (2 mentions)
H3k27me3 antibody, by Abcam (2 mentions)
Sall4 antibody, by Abcam (2 mentions)
Stat3 antibody, by Santa Cruz Biotechnology (2 mentions)
Oct4 antibody, by Abcam (2 mentions)
4 protocols using brg1 antibody
1
Chromatin Immunoprecipitation Protocol
2
Chromatin Immunoprecipitation Protocol
3
Immunohistochemical Analysis of BRG1 in ALCL
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Immunohistochemistry was conducted as described previously [25 (link)]. In brief, a tissue microarray containing cores of 12 ALK+ ALCL and 9 ALK- ALCL FFPE patient samples as well as 8 Peripheral T-cell Lymphoma—Not Otherwise Specified (PTCL-NOS) and 6 Angioimmunoblastic T-cell Lymphoma (AITL) patient samples was subjected to antigen retrieval following incubation in a citrate buffer before exposure to BRG1 antibody (Abcam, Cambridge, UK) diluted 1:100 in PBS/BSA overnight at 4 °C. Tumours were defined as being highly positive for BRG1 (++) if >30% cells stained highly positive and BRG1 + if >30% tumour cells stained positive for BRG1.
4
Western Blot Analysis of BRG1, KSHV, and Actin
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The BRG1 antibody (#4081) was purchased from Abcam. Monoclonal antibodies against KSHV, ORF45, and PF8 were described previously (Zhu et al. 2005 (link)). A monoclonal antibody against beta-actin was purchased from Sigma-Aldrich. Whole-cell extract equivalent to 0.1 × 106 cells was resolved on SDS-PAGE 10% Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes. The membranes were blocked in 5% dried milk in PBS containing 0.2% Tween 20 (PBST) followed by an overnight incubation with diluted primary antibodies at 4°C. Anti-rabbit or anti-mouse immunoglobulin G antibody conjugated to horseradish peroxidase was used as the secondary antibody. The SuperSignal West Dura Chemiluminescent Substrate (Pierce) was used for detection of antibody–antigen complexes.
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