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Donkey anti sheep igg hrp

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Donkey anti-sheep IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and quantify the presence of sheep immunoglobulin G (IgG) in various experimental and analytical applications.

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Lab products found in correlation

Anti ptprz, by Santa Cruz Biotechnology (1 mentions) Ab9015, by Abcam (1 mentions) Ab16505, by Abcam (1 mentions) Anti ptprz1, by Merck Group (1 mentions) Hpa015103, by Merck Group (1 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Horse anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Alexa fluor 594 anti rabbit, by Thermo Fisher Scientific (1 mentions) P tyr 100, by Cell Signaling Technology (1 mentions) Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions) Vinculin e1e9v, by Cell Signaling Technology (1 mentions) Ak 15, by Merck Group (1 mentions)

2 protocols using donkey anti sheep igg hrp

1

Immunohistochemical Characterization of PTPRζ

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Most chemicals were purchased from Sigma or Wako Chemicals. The following antibodies were purchased: mouse IgM anti-PTPRζ (sc-33664; Santa Cruz Biotechnology), hereinafter referred to as “anti-PTPRZ (Santa Cruz),” mouse IgM anti-chondroitin sulfate proteoglycan (MAB1581; Merck Millipore), referred to by its clone name, as “Cat-315,” mouse monoclonal anti-aggrecan (6-B-4; Abcam), rabbit polyclonal anti-PTPRZ1(HPA015103; Sigma), hereinafter referred to as “anti-PTPRZ1 (Sigma),” anti-pan cadherin (ab16505; Abcam), polyclonal sheep anti-transthyretin (ab9015; Abcam), referred to as “TTR,” horseradish peroxidase (HRP)-conjugated goat anti-mouse IgM (SAB-110; Stressgen), goat anti-rabbit IgG (NA934; GE Healthcare), and donkey anti-sheep IgG-HRP (A16041; Thermo Fisher Scientific).
Yamanoi Y., Fujii M., Murakami Y., Nagai K., Hoshi K., Hashimoto Y., Honda T., Saito K, & Kitazume S. (2020). Soluble protein tyrosine phosphatase receptor type Z (PTPRZ) in cerebrospinal fluid is a potential diagnostic marker for glioma. Neuro-oncology Advances, 2(1), vdaa055.
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2

Phosphorylation Detection in Cell Biology

Cited 3 times
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For KiPIK screening, immunoblotting and immunofluorescence microscopy, rabbit polyclonal antibodies to H3T3ph (B8634)47 (link), INCENP (P240, Cell Signaling Technology #2807), INCENP-S446ph (Jan-Michael Peters, IMP, Vienna)57 (link), INCENP-TSSph (Michael Lampson, University of Pennsylvania)82 (link), BCL9L S915ph (Cell Signaling Technology #13325), Neurogranin S36ph (Merck-Millipore, ABN426) and γ-tubulin (AK-15, Sigma, T3320); rabbit monoclonal antibodies to the S-pT-P motif (D73F6; Cell Signaling Technology #5243), Cyclin B1 (D5C10; Cell Signaling Technology #12231) and Vinculin (E1E9V; Cell Signaling Technology #13901); mouse monoclonal antibodies to H3S28ph (CMA315)83 (link) and phospho-tyrosine (P-Tyr-100; Cell Signaling Technology #9411); and sheep polyclonal antibodies to Aurora B (SAB.1, Stephen Taylor, University of Manchester)84 (link) and BCL9L (R&D Systems, AF4967) were used. Antibodies used for siRNA screening were mouse monoclonal anti-H3T3ph (16B2)85 (link) and rabbit polyclonal anti-H2BS6ph86 (link). Secondary antibodies were: donkey anti-sheep IgG-HRP (ThermoFisher, A16041), goat anti-rabbit IgG-HRP (Cell Signaling Technology #7074), horse anti-mouse IgG-HRP (Cell Signaling Technology #7076), donkey anti-mouse Alexa Fluor 488, anti-rabbit Alexa Fluor 594 and anti-sheep Alexa 488 Fluor (ThermoFisher, A-21202, A-21207, and A-11015).
Watson N.A., Cartwright T.N., Lawless C., Cámara-Donoso M., Sen O., Sako K., Hirota T., Kimura H, & Higgins J.M. (2020). Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites. Nature Communications, 11, 1684.
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