Dm rxe microscope

Manufactured by Leica

Sourced in Germany

The Leica DM RXE is a research-grade microscope designed for advanced scientific applications. It features a high-quality optical system, precise focusing mechanisms, and versatile illumination options. The core function of the Leica DM RXE is to provide clear, detailed, and magnified images of specimens for observation, analysis, and documentation.

Automatically generated - may contain errors

Lab products found in correlation

Tcs sp2 aobs confocal system, by Leica (11 mentions) Lsm 710, by Zeiss (2 mentions) Spectracube, by Applied Spectral Imaging (1 mentions) Wheat germ agglutinin (wga), by Vector Laboratories (1 mentions) Syto59, by Thermo Fisher Scientific (1 mentions) Live dead baclight, by Thermo Fisher Scientific (1 mentions) Concanavalin a (cona), by Vector Laboratories (1 mentions) Syto 59, by Merck Group (1 mentions) Nile red, by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions) Isomet 1000, by Buehler (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions) Af555, by Thermo Fisher Scientific (1 mentions) Qwin image analysis system, by Leica (1 mentions) Mec13, by BD (1 mentions) Dm5000, by Leica (1 mentions) Vectastain elite abc kit, by Vector Laboratories (1 mentions) Tissue tek, by Sakura Finetek (1 mentions)

Spelling variants of this product

DM RXE confocal microscope (4 citations)

20 protocols using dm rxe microscope

Hemostatic Agents Impact on Bacterial Attachment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 19

To determine the impact, if any, of a hemostatic agent, such as, for example, one or more of hemostatic agents 24 on bacterial attachment, three samples were prepared. The first sample included a TYRX polymer in the p22-xx family (P22-27.5-TYRX); the second sample included TYRX and tranexamic acid (TYRX+TXA); and the third sample included an extracellular matrix (ECM) to be used as a control. In this example, TYRX refers to a glycoprene mesh that is coated with P22-27.5 (a polymer in the P22-X family) containing rifampin and minocylcline. The samples were each suspended in 3 mL of a Brain Heart Infusion (BHI) medium at 37° C. for 24 hours. The samples were each inoculated with 2×Colony Forming Units (CFU)/mL of a clinical strain of Methicillin-resistant Staphylococcus aureus (MRSA). After 24 hours, each of the samples was rinsed twice and bacterial attachment was visualized by Live/Dead staining and imaged with Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, Pa.). As shown in FIG. 25, the TYRX and TYRX+TXA samples exhibited less bacterial attachment, with the TYRX+TXA sample exhibiting less bacterial attachment than the TYRX sample.

US10939901B2. Customizable anchorage devices and methods of use (2021-03-09). TYRX, Inc. [US]. Inventors: Satish Pulapura [US], Fatima Buevich [US], Archana Rajaram [US], Jonathan M. Mahoney [US], Dan Thanh Le [US].

+ Open protocol

+ Expand

Hemostatic Agents and Bacterial Attachment

Check if the same lab product or an alternative is used in the 5 most similar protocols

EXAMPLE 19

To determine the impact, if any, of a hemostatic agent, such as, for example, one or more of hemostatic agents 24 on bacterial attachment, three samples were prepared. The first sample included a TYRX polymer in the p22-xx family (P22-27.5-TYRX); the second sample included TYRX and tranexamic acid (TYRX+TXA); and the third sample included an extracellular matrix (ECM) to be used as a control. In this example, TYRX refers to a glycoprene mesh that is coated with P22-27.5 (a polymer in the P22-X family) containing rifampin and minocylcline. The samples were each suspended in 3 mL of a Brain Heart Infusion (BHI) medium at 37° C. for 24 hours. The samples were each inoculated with 2×Colony Forming Units (CFU)/mL of a clinical strain of Methicillin-resistant Staphylococcus aureus (MRSA). After 24 hours, each of the samples was rinsed twice and bacterial attachment was visualized by Live/Dead staining and imaged with Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, Pa.). As shown in FIG. 42, the TYRX and TYRX+TXA samples exhibited less bacterial attachment than the ECM control.

US11577010B2. Hemostatic devices and methods of use (2023-02-14). MEDTRONIC INC. [US]. Inventors: Satish Pulapura [US], Fatima Buevich [US], Jorie S. Soskin [US], Franklin R. Mansfield [US], Kai R. Worrell [US], Charlie Wood [US], Jorge Alberto Trevino Blanco [US].

+ Open protocol

+ Expand

Preparation and Imaging of SKY Chromosome Spreads

Check if the same lab product or an alternative is used in the 5 most similar protocols

Preparation of SKY probes, slide pre-treatment, slide denaturation, detection, and imaging have been described previously31 (link). Protocols can be accessed at: http://www.riedlab.nci.nih.gov/protocols. Metaphase chromosome suspensions were prepared by treating cells with a hypotonic solution (0.075 M KCl), followed by methanol: acetic acid (3:1, vol/vol) fixation. The suspension was then dropped onto slides using a Thermatron™ to control humidity. The slides were aged at 37 °C for approximately one week prior to hybridization. Chromosome preparations were hybridized with SKY probes (prepared in-house) for 72 hours. Slides were imaged for SKY analysis using a Leica DMRXE microscope (Leica, Germany) equipped with DAPI and SKY™ filters (Chroma, Bellows Falls, VT), a Xenon lamp, and Spectracube™ (Applied Spectral Imaging, Vista, CA). Spectrum-based classification and analysis of the fluorescent images (SKY) was achieved using SkyViewTM software (Applied Spectral Imaging). Approximately 15–20 metaphase spreads were acquired for SKY analysis for each cell line and scored for numerical and structural chromosomal aberrations according to established human chromosome nomenclature rules from ISCN (2009)[2].

Yuan H., Krawczyk E., Blancato J., Albanese C., Zhou D., Wang N., Paul S., Alkhilaiwi F., Palechor-Ceron N., Dakic A., Fang S., Choudhary S., Hou T.W., Zheng Y.L., Haddad B.R., Usuda Y., Hartmann D., Symer D., Gillison M., Agarwal S., Wangsa D., Ried T., Liu X, & Schlegel R. (2017). HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes. Scientific Reports, 7, 45617.

+ Open protocol

+ Expand

FISH-Based Bacterial Identification Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of the ACL samples were fixed with fresh 4 % paraformaldehyde and incubate for 2–4 h at 4 °C. After the incubation the specimen was spun down and the supernatant removed, this process was repeated twice with Hank’s Salt Saline Solution (HBSS). Next, the samples were resuspended in 50 % Ethanol-PBS solution and stored at −20 °C for evaluation with FISH. FISH was performed as described by Nistico et al. (Nistico et al. 2014 (link)), using species-specific and genus-specific fluorescent 16 s rRNA probes. The bacteria targeted and probe sequences selected were: (1) Streptococcus “GTG ATG CAA GTG CAC CTT” (Kempf et al. 2000 (link)); (2) Staphylococcus sp “TCC TCC ATA TCT CTG CGC” (Trebesius et al. 2000 (link)); and (3) Lactobacillus sp “CCATTGTGGAAGATTCCCT” (Quevedo et al. 2011 (link)).
Samples were observed with Confocal Scanning Laser Microscopy (CSLM) imaging using a Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, PA) using a 63X (NA1.2) water immersion lens.

Hiller N.L., Chauhan A., Palmer M., Jain S., Sotereanos N.G., Altman G.T., Nistico L., Kreft R., Post J.C, & Demeo P.J. (2015). Presence of bacteria in failed anterior cruciate ligament reconstructions. SpringerPlus, 4, 460.

+ Open protocol

+ Expand

Antimicrobial Effect of Hemostatic Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 19

To determine the impact, if any, of a hemostatic agent, such as, for example, one or more of hemostatic agents 24 on bacterial attachment, three samples were prepared. The first sample included a TYRX polymer in the p22-xx family (P22-27.5-TYRX); the second sample included TYRX and tranexamic acid (TYRX+TXA); and the third sample included an extracellular matrix (ECM) to be used as a control. In this example, TYRX refers to a glycoprene mesh that is coated with P22-27.5 (a polymer in the P22-X family) containing rifampin and minocycline. The samples were each suspended in 3 mL of a Brain Heart Infusion (BHI) medium at 37° C. for 24 hours. The samples were each inoculated with 2×Colony Forming Units (CFU)/mL of a clinical strain of Methicillin-resistant Staphylococcus aureus (MRSA). After 24 hours, each of the samples was rinsed twice and bacterial attachment was visualized by Live/Dead staining and imaged with Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, Pa.). As shown in FIG. 29, the TYRX and TYRX+TXA samples exhibited less bacterial attachment than ECM control.

US10980922B2. Hemostatic devices and methods of use (2021-04-20). MEDTRONIC, INC. [US]. Inventors: Satish Pulapura [US], Fatima Buevich [US], Jorie S. Soskin [US], Franklin R. Mansfield [US], Kai R. Worrell [US], Charlie Wood [US], Jorge Alberto Trevino Blanco [US], Narvel M. Brooks, III [US].

+ Open protocol

+ Expand

Fluorescence in Situ Hybridization for Bacterial Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of the tissue specimens were fixed with fresh 4 % paraformaldehyde and incubated for 2–4 h at 4 °C. After the incubation, the specimen was centrifuged and the supernatant removed. This process was repeated twice with Hank’s Salt Saline Solution (HBSS). Next, the samples were resuspended in 50 % ethanol-PBS solution and stored at −20 °C for evaluation with FISH. FISH was performed as described by Nistico et al. (41) using species-specific fluorescent 16 s rRNA probes. Escherichia coli (E. coli) was targeted using probe sequence “GCA TAA GCG TCG CTG CCG” [22 (link)] and Streptococcus pneumonia (S. pneumonia) was targeted using “GTG ATG CAA GTG CAC CTT” [23 (link)].
Samples were observed with Confocal Scanning Laser Microscopy (CSLM) imaging using a Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, PA) using a 63X (NA1.2 ) water immersion lens.

Zaidi A.H., Kelly L.A., Kreft R.E., Barlek M., Omstead A.N., Matsui D., Boyd N.H., Gazarik K.E., Heit M.I., Nistico L., Kasi P.M., Spirk T.L., Byers B., Lloyd E.J., Landreneau R.J, & Jobe B.A. (2016). Associations of microbiota and toll-like receptor signaling pathway in esophageal adenocarcinoma. BMC Cancer, 16, 52.

+ Open protocol

+ Expand

Fractal Dimension Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

To calculate the CI, 64 tumor samples were randomly selected for computer image analysis from one patient group used for the CTGF SNP study. Slide preparations, including sectioning, staining and image processing were performed using the methodology as described by Franzén et al (30 (link)). In brief, images from the invasive front of the tumor area were captured using a Leica DC200 digital camera mounted on a Leica DMRXE microscope with 10X objective lens (Leica Microsystems GmbH, Wetzlar, Germany). From each sample, an average of 7 (range of 5–10) images were captured. The number of images depended upon the length of the tumor-stromal area. Images were adjusted so that the tumor area appeared black and the background white. These images were used to calculate the number of free tumor cells and tumor cell clusters. The black color was then removed so that only the outline of tumor remained (40 (link)). Using the tumor outline image, the fractile dimensions were calculated using various software programs; Adobe Photoshop, version 7.0 (Adobe Systems, Inc., San Jose, CA, USA) with the Fovea Pro (Reindeer Graphics, Inc., Asheville, NC, USA) was used for the black/white and the tumor outline images, and ImageJ software (http://imagej.nih.gov/ij/) was used to calculate the fractal dimension value. The CI (ranges 1–5) was obtained by calculating the mean value of these parameters.

AHMAD A., ASKARI S., BEFEKADU R, & HAHN-STRÖMBERG V. (2014). Investigating the association between polymorphisms in connective tissue growth factor and susceptibility to colon carcinoma. Molecular Medicine Reports, 11(4), 2493-2503.

+ Open protocol

+ Expand

Multimodal Imaging of Microbial Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Material from the upper test tube volume and the test tube bottom of 14-day old cultures (#49134 and MH strain) was removed by gentle suction using a cut-off 1 mL pipette tips (to minimize shearing), and labeled with LIVE/DEAD BacLight (Invitrogen, Carlsbad, CA, USA) according to the manufactures instructions. In the same way, more 14-day old cultures were stained with Concanavalin A (25 µg mL⁻¹, (ConA, Vector Laboratories, Inc., Burlingame, CA, USA) and Syto59 (5 µM, Invitrogen, Carlsbad, CA, USA), or Wheat Germ Agglutinin (25 µg mL⁻¹) (Vector Laboratories, Inc.) and Syto59, or Sypro Orange and Syto59, or Nile Red (5 µg mL⁻¹, Sigma-Aldrich Corp. St. Louis, MO, USA), or Thioflavin T (20 mM, Sigma-Aldrich). Fluorescence in situ hybridization (FISH) was performed on material collected from the test tube bottom, which was hybridized with the EUB338-Cy3 probe [34] (link) or the NONEUB-Cy5 probe [35] (link) at a final concentration of 5 ng µL⁻¹ for 90 min at 46°C. These specimens were counter-stained with Syto59. All samples were examined either with a Leica DM RXE microscope with a TCS SP2 AOBS confocal system (Leica Microsystem, Exton, PA, USA) or a LSM710 (Carl Zeiss MicroImaging, Inc., Thornwood, NY, USA.) using confocal and transmitted imaging with the appropriate laser wavelengths lines and detection windows.

Schaudinn C., Stoodley P., Hall-Stoodley L., Gorur A., Remis J., Wu S., Auer M., Hertwig S., Guerrero-Given D., Hu F.Z., Ehrlich G.D., Costerton J.W., Robinson D.H, & Webster P. (2014). Death and Transfiguration in Static Staphylococcus epidermidis Cultures. PLoS ONE, 9(6), e100002.

+ Open protocol

+ Expand

Evaluating Hemostatic Agents' Impact on Bacterial Attachment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 19

To determine the impact, if any, of a hemostatic agent, such as, for example, one or more of hemostatic agents 24 on bacterial attachment, three samples were prepared. The first sample included TYRX, a polymer in the p22-xx family (P22-27.5-TYRX); the second sample included TYRX and tranexamic acid (TYRX+TXA); and the third sample included an extracellular matrix (ECM) to be used as a control. In this example, TYRX refers to a glycoprene mesh that is coated with P22-27.5 (a polymer in the P22-X family) containing rifampin and minocycline. The samples were each suspended in 3 mL of a Brain Heart Infusion (BHI) medium at 37° C. for 24 hours. The samples were each inoculated with 2× Colony Forming Units (CFU)/mL of a clinical strain of Methicillin-resistant Staphylococcus aureus (MRSA). After 24 hours, each of the samples was rinsed twice and bacterial attachment was visualized by Live/Dead staining and imaged with Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, Pa.). As shown in FIG. 26, the TYRX and TYRX+TXA samples exhibited less bacterial attachment than the ECM control.

US10765782B2. Hemostatic devices and methods of use (2020-09-08). TYRX, Inc. [US]. Inventors: Satish Pulapura [US], Fatima Buevich [US], Jorie S. Soskin [US], Franklin R. Mansfield [US], Kai R. Worrell [US], Charlie Wood [US], Jorge Alberto Trevino Blanco [US].

+ Open protocol

+ Expand

Evaluating Hemostatic Agents on Bacterial Attachment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 19

To determine the impact, if any, of a hemostatic agent, such as, for example, one or more of hemostatic agents 24 on bacterial attachment, three samples were prepared. The first sample included TYRX, a polymer in the p22-xx family (P22-27.5-TYRX); the second sample included TYRX and tranexamic acid (TYRX+TXA); and the third sample included an extracellular matrix (ECM) to be used as a control. In this example, TYRX refers to a glycoprene mesh that is coated with P22-27.5 (a polymer in the P22-X family) containing rifampin and minocycline. The samples were each suspended in 3 mL of a Brain Heart Infusion (BHI) medium at 37° C. for 24 hours. The samples were each inoculated with 2× Colony Forming Units (CFU)/mL of a clinical strain of Methicillin-resistant Staphylococcus aureus (MRSA). After 24 hours, each of the samples was rinsed twice and bacterial attachment was visualized by Live/Dead staining and imaged with Leica DM RXE microscope attached to a TCS SP2 AOBS confocal system (Leica Microsystems, Exton, Pa.). As shown in FIG. 26, the TYRX and TYRX+TXA samples exhibited less bacterial attachment than the ECM control.

US11471570B2. Hemostatic devices and methods of use (2022-10-18). MEDTRONIC, INC. [US]. Inventors: Satish Pulapura [US], Fatima Buevich [US], Jorie S. Soskin [US], Franklin R. Mansfield [US], Kai R. Worrell [US], Charlie Wood [US], Jorge Alberto Trevino Blanco [US], Narvel M. Brooks, III [US].

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!