Circular dichroism measurements were carried out using J-725 CD spectropolarimeter (JASCO). CD titration spectra of (CAG)9 (2.5 μM) with variable concentration of CMBL3a (2.5, 5.0, 10, 15, 20, 25, 35, 45, 50 and 55 μM) were measured at ambient temperature in 10 mM sodium cacodylate pH 7.0 and 100 mM NaCl. The apparent dissociation constant (Kd) of (CAG)9-CMBL3a complex was calculated by fitting the data to the 1:2 binding isotherm. RNA oligonucleotides were purchased from Gene Design Inc.
Rna oligonucleotides
Manufactured by Ajinomoto Group
Sourced in Japan
RNA oligonucleotides are synthetic molecules composed of ribonucleic acid (RNA) monomers. They serve as versatile laboratory tools for various applications, including gene expression analysis, RNA interference (RNAi), and other molecular biology techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rna oligonucleotides
1
Circular Dichroism Characterization of (CAG)9-CMBL3a
2
Synthesis and Annealing of siRNA Duplexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA oligonucleotides corresponding
to the guide and passenger strands
of each siRNA were chemically synthesized (Gene Design, Osaka, Japan;
Shanghai Gene Pharma, Shanghai, China) and subsequently annealed to
form endogenous siRNA duplexes. All siRNA sequences used in this study
are summarized inSupporting Information Table S2.
to the guide and passenger strands
of each siRNA were chemically synthesized (Gene Design, Osaka, Japan;
Shanghai Gene Pharma, Shanghai, China) and subsequently annealed to
form endogenous siRNA duplexes. All siRNA sequences used in this study
are summarized in
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