Cd79a

Manufactured by Santa Cruz Biotechnology

Sourced in United States

CD79A is a cell surface receptor expressed by B cells. It is a component of the B cell antigen receptor complex, which plays a critical role in B cell activation and differentiation.

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Lab products found in correlation

7 protocols using cd79a

Western Blot Analysis of BCR Signaling

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Luxeptinib was provided by Aptose Biosciences. Ibrutinib (#HY-10997) was purchased from MedChem Express. Goat anti-human IgM (#109-005-043) was purchased from Jackson ImmunoResearch. Human lymphoma cell lines SU-DHL-6 (#CRL-2959), JeKo-1 (#CRL-3006), RL (#CRL-2261) and Fetal Bovine Serum (#30–2020) were obtained from ATCC. SU-DHL-6 is a human lymphoblast-like cell line, JeKo-1 is a mantle cell lymphoma cell line and RL is a human non-Hodgkin’s lymphoma B cell line. RPMI-1640 medium (#11875–093) and penicillin/streptomycin (#15070063) were purchased from Thermo Fisher Scientific. Antibodies against p-BTK (Y223) (#87141), BTK (#56044), p-PLCγ2 (#3871), p-SYK (#2710), SYK (#80460), p-BLNK (#3601), BLNK (#36438), p-CD79A (#5173), p-LYN/LCK/HCK/BLK (#70926), p-LYN (Y507) (#2731), LYN (#2796, #4576), pSrc family (#6943) and GAPDH (#2118) were purchased from Cell Signaling Technology. Phospho-BTK (Y551) (#ab40770) was purchased from Abcam. PLCγ2 (#sc-5283) and CD79A (#sc-20064) were purchased from Santa Cruz Biotechnology. All other reagents and chemicals used were of analytical grade and were obtained from Sigma-Aldrich or Thermo Fisher Scientific.

Sonowal H., Rice W.G, & Howell S.B. (2023). Luxeptinib interferes with LYN-mediated activation of SYK and modulates BCR signaling in lymphoma. PLOS ONE, 18(3), e0277003.

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Phenotypic Characterization of Macrophages and PBMSCs

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Target cells were adjusted to a density of 1.0 × 10⁶/mL, and 100 μL of cell suspension was transferred into flow tubes. The primary antibodies used were as follows: mouse anti-rat CD11b (BD, New Jersey, USA) and CD68 (Santa Cruz, Dallas, Texas, USA), and mouse IgA (BD) and IgG2b (BD), and were used for the identification of M0 macrophages; mouse anti-rat CD29 (eBioscience, San Diego, California, USA), CD90 (eBioscience), CD44 (Santa Cruz), CD79a (Santa Cruz), CD45 (eBioscience), and CD11b (BD) were used for the identification of PBMSCs; and mouse anti-rat CD206 (Santa Cruz) and CD86 (Santa Cruz), and mouse IgG1 (BD) and IgG2b (BD) were used for the identification of M1 and M2 macrophages. After 30 min of incubation with primary antibodies, cells were subsequently incubated with Alexa Fluor-conjugated rabbit or goat anti-mouse IgG secondary antibodies. After washing with PBS and fixing with 200 μL 4% paraformaldehyde, cell samples were analyzed by FACSCalibur flow cytometry (Becton Dickinson, USA), and data were analyzed using Cell Quest software.

Pang Q.M., Yang R., Zhang M., Zou W.H., Qian N.N., Xu Q.J., Chen H., Peng J.C., Luo X.P., Zhang Q, & Zhang T. (2022). Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway. Stem Cells International, 2022, 5181241.

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Gingival Tissue Protein Profiling

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Total protein (20-40 μg) from human gingival tissues (n=4) was separated by 10% SDS–PAGE and then transferred onto a 0.45-μm polyvinylidene difluoride membrane (Millipore) at 250 mA for 2 h on ice. The membrane was blocked with 5% skim milk dissolved in Tris-buffered saline containing 0.05% Tween 20 and probed with primary antibodies against CD38 (cat# sc-374650), CD79A (cat# sc-20064), ADPGK (cat# sc-100751) were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA), OGN (cat# 12755-1-AP), HLA-DPA1 (cat# 16109-1-AP), PLCH1 (cat# 19143-1-AP), GAPDH (cat# 10494-1-AP) were purchased from Proteintech (Wuhan, Hubei, P.R.C), TMED5 (cat# SRP08852), GSTCD (cat# SRP11511) were purchased from Saier Biotechnology (Tianjin, P.R.C) and TBXAS1 (cat# ab157481) was purchased from Abcam (Cambridge, MA, USA) at the indicated dilutions overnight at 4°C. The membrane was then incubated with horseradish peroxidase (HRP)–conjugated secondary antibodies (anti-rabbit, cat# B900210 and anti-mouse, cat#SA00001-1) were purchased from Proteintech (Wuhan, Hubei, P.R.C) and detected by enhanced chemiluminescence reagents (Biosharp Life Sciences) using an image analyser. Levels of target proteins were normalized to GAPDH, which served as a reference control. The intensity of the protein bands was analyzed with ImageJ software, and the values are expressed as the mean ± standard deviation (SD).

Liu W., Qiu W., Huang Z., Zhang K., Wu K., Deng K., Chen Y., Guo R., Wu B., Chen T, & Fang F. (2022). Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics. Frontiers in Immunology, 13, 963123.

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Quantitative Immunohistochemical Analysis of the Local Immune Response to High-Dose H-FIRE Ablation

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The local immune response to treatment was assessed by characterizing immune cell infiltrates within brain sections collected from high-dose H-FIRE ablation groups at the study endpoint. Tissue samples were sectioned and stained for presence of T cells (CD3, Dako), helper T cells (CD4, Origene), cytotoxic T cells (CD8, Invitrogene), regulatory T cells (FoxP3, Invitrigene), B cells (CD79a, Santa Cruz), microglia (IBA-1, FUJIFILM), M1 macrophages (CD86, Abcam), and M2 macrophages (CD163, Abcam). Methods were followed as presented by Koshkaki et al. (35 (link)). Samples were categorized as peritumoral region (healthy tissue), transition zone (submillimeter zone between the tumor mass and the peritumoral region), tumor mass, and the necrotic core. Tissue samples were imaged using a Nikon Eclipse Ni-U microscope using a Ds-Ri2 camera. The acquired images were analyzed using the NIS element BR software version 5.21.01.
Briefly, auto thresholding was done on all images followed by greyscale conversion. Mean gray values (MGVi) and gray area fractions (AF) were calculated using the software. The values were then used to find the final chromogen intensity (f) as follows:
Mean f and AF values were next calculated using five high power fields from each slide. The immunohistochemistry (IHC) score was calculated using the mean values and the following equation:

Campelo S.N., Lorenzo M.F., Partridge B., Alinezhadbalalami N., Kani Y., Garcia J., Saunier S., Thomas S.C., Hinckley J., Verbridge S.S., Davalos R.V, & Rossmeisl JH J.r. (2023). High-frequency irreversible electroporation improves survival and immune cell infiltration in rodents with malignant gliomas. Frontiers in Oncology, 13, 1171278.

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Antibody Validation for Western Blot and ChIP

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Antibodies for western blotting and ChIP were HMGN1 (Bethyl, 302-363A), BCL6 (Cell Signaling Technologies, 14895S), beta-actin (Sigma, SAB5500001), CD79A (Santa Cruz, sc-25604), LYN (Santa Cruz, sc-15), SYK (Santa Cruz, sc12-40), H3K27me3 (Cell Signaling Technologies, 9733), H3K4me3 (Abcam, ab8580), H3K27ac (Abcam, ab4729), or control IgG (Cell Signaling Technologies, 2729S).

Mowery C.T., Reyes J.M., Cabal-Hierro L., Higby K.J., Karlin K.L., Wang J.H., Kimmerling R.J., Cejas P., Lim K., Li H., Furusawa T., Long H.W., Pellman D., Chapuy B., Bustin M., Manalis S.R., Westbrook T.F., Lin C.Y, & Lane A.A. (2018). Trisomy of a Down Syndrome Critical Region Globally Amplifies Transcription via HMGN1 Overexpression. Cell reports, 25(7), 1898-1911.e5.

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Antibody Validation for Western Blot and ChIP

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Flow Cytometry Analysis of BMSCs

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BMSCs and MMP1-BMSC phenotypes were analyzed by flow cytometry using a FACSCalibur (Becton-Dickinson Biosciences, Ann Arbor, MI, USA). The cells were re-suspended in phosphate-buffered saline (PBS) at a concentration of 1×10⁶ cells/ml, and were incubated with following fluorescent anti-human antibodies: fluorescein isothiocyanate (FITC)-conjugated CD45 and CD90, phycoerythrin (PE)-conjugated CD105, CD14, CD34 and CD79a (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). The rat immunoglobulin IgG-FITC and IgG-PE was used as the isotype-matched control. The cells were tagged 45 min away from light at room temperature, washed three times with PBS and detected with FACSCalibur flow cytometer.

Du C., Jiang M., Wei X., Qin J., Xu H., Wang Y., Zhang Y., Zhou D., Xue H., Zheng S, & Zeng W. (2018). Transplantation of human matrix metalloproteinase-1 gene-modified bone marrow-derived mesenchymal stem cell attenuates CCL4-induced liver fibrosis in rats. International Journal of Molecular Medicine, 41(6), 3175-3184.

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